https://m2.mtmt.hu/ hun 2026-09-23 00:34 JournalArticle 32211838 VALIDATED true 0 false 2026-05-21T04:07:23.815+0000 2022-10-14T09:04:24.132+0000 2021-09-17T14:35:58.874+0000 true 10015546 Berhidi Anna /api/admin/10015546 Admin true 10015546 2022-10-14T09:04:10.767+0000 2022-10-14T09:04:10.767+0000 2022-10-14T09:04:29.455+0000 true 10015544 Csajbók Edit /api/admin/10015544 Admin true 10015544 true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Birk, Julia Monitoring Changes in the Oxidizing Milieu in the Endoplasmic Reticulum of Mammalian Cells Using HyPerER true true /api/journal/10066107 REVIEWED true false 10066107 false 10066107 true 2331-8325 2331-8325 Journal FOREIGN 11 13 e4076 e4076 8 2021 The production of reactive oxygen species (ROS) and endoplasmic reticulum (ER) stress are tightly linked. The generation of ROS can be both the cause and a consequence of ER stress pathways, and an increasing number of human diseases are characterized by tissue atrophy in response to ER stress and oxidative injury. For the assessment of modulators of ER luminal ROS generation and for mechanistic studies, methods to monitor changes in ER reduction-oxidation (redox) states in a time-resolved and organelle-specific manner are needed. This has been greatly facilitated by the development of genetically encoded fluorescent probes, which can be targeted to different subcellular locations by specific amino acid extensions. One of these probes is the yellow fluorescent protein-based redox biosensor, HyPer. Here, we provide a protocol for the time-resolved monitoring of the oxidizing milieu in the ER of adherent mammalian cells using the ratiometric sensor, HyPerER, which is specifically targeted to the ER lumen. 2021 true true true false false GREEN 2026-05-21 true http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8292116 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2 false 10026029 false false false 2021-09-16T14:42:53.796+0000 Molekuláris Biológiai Tanszék (SE / AOK / I / BMBI) 1 6 1 4 0 false Language 10002 /api/language/10002 Angol English true 2 true PersonAuthorship 97513176 /api/authorship/97513176 1 0.25 true false false Birk Julia true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 97513177 /api/authorship/97513177 2 0.25 false false false Author 10026029 /api/author/10026029 Lizák Beáta true 10026029 true Lizak Beata true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 97513178 /api/authorship/97513178 3 0.25 false false false Appenzeller-Herzog Christian true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 97513179 /api/authorship/97513179 4 0.25 false true true Odermatt Alex true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PublicationIdentifier 19314047 /api/publicationidentifier/19314047 PlainSource 6 /api/publicationsource/6 PublicationSourceType 10001 /api/publicationsourcetype/10001 true true true DOI DOI https://doi.org/@@@ true true 6 true PAY IDENTICAL 10.21769/BioProtoc.4076 https://doi.org/10.21769/BioProtoc.4076 false true PublicationIdentifier 19314048 /api/publicationidentifier/19314048 PlainSource 1 /api/publicationsource/1 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true WoS WoS https://www.webofscience.com/wos/woscc/full-record/@@@ true true 1 true IDENTICAL 000687826100009 https://www.webofscience.com/wos/woscc/full-record/000687826100009 false true PublicationIdentifier 19314049 /api/publicationidentifier/19314049 PlainSource 17 /api/publicationsource/17 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true PubMed PubMed http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=@@@&dopt=Abstract true true 17 true IDENTICAL 34327273 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=34327273&dopt=Abstract false true PublicationIdentifier 19314128 /api/publicationidentifier/19314128 PlainSource 137 /api/publicationsource/137 PublicationSourceType 10007 /api/publicationsourcetype/10007 true true true Pubmed Central http://www.ncbi.nlm.nih.gov/pmc/articles/@@@ true true 137 true GREEN true PMC8292116 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8292116 false true Keyword 4978 /api/keyword/4978 true 4978 true Keyword 13954 /api/keyword/13954 true 13954 true Keyword 1031208 /api/keyword/1031208 true 1031208 true Keyword 1052194 /api/keyword/1052194 true 1052194 true Keyword 1395885 /api/keyword/1395885 true 1395885 true Keyword 2014641 /api/keyword/2014641 true true /api/publication/32211838 <div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" >Birk Julia </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10026029"><a href="/gui2/?type=authors&mode=browse&sel=10026029" target="_blank">Lizak Beata (<span class="authorship-author-name">Lizák Beáta</span> <span class="authorAux-mtmt"> biokémia, molekuláris biológia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/<span title="Biokémiai és Molekuláris Biológiai Intézet">BMBI</span>/Molekuláris Biológiai Tanszék</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Appenzeller-Herzog Christian </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Odermatt Alex ✉ </span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;32211838" target="_blank">Monitoring Changes in the Oxidizing Milieu in the Endoplasmic Reticulum of Mammalian Cells Using HyPerER</a></div> <div> <span class="journal-title">BIO-PROTOCOL</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/2331-8325">2331-8325</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/2331-8325">2331-8325</a>)</span>: <span class="journal-volume">11</span> <span class="journal-issue">13</span> <span class="page"> Paper e4076. 8 p. </span> <span class="year">(2021)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Nyelv: Angol | </span> <span class="identifiers"> <span class="id identifier oa_PAY" title=" Fizetős "> <a style="color:blue" title="10.21769/BioProtoc.4076" target="_blank" href="https://doi.org/10.21769/BioProtoc.4076"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="000687826100009" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/000687826100009"> WoS </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="34327273" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=34327273&dopt=Abstract"> PubMed </a> </span> <span class="id identifier oa_GREEN" title=" Green "> <a style="color:black" title="PMC8292116" target="_blank" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8292116"> Pubmed Central </a> </span> </span> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;32211838&sort=publishedYear,desc&sort=title"> Idézett közlemények száma: 2 </a> </div> <div class="mtid"><span class="long-pub-mtid">Közlemény: 32211838</span> | <span class="status-data status-VALIDATED"> Egyeztetett </span> Forrás Idéző | <span class="type-subtype">Folyóiratcikk ( Szakcikk ) </span> | <span class="pub-category">Tudományos</span> | <span class="publication-sourceOfData">WOS TAGGED</span> </div> <div class="lastModified">Utolsó módosítás: 2022.10.14. 11:04 Csajbók Edit (SE_KK_Admin5_CSE, admin) </div> </div></div>