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      <abstractText>In this study we tested whether a protein corona is formed around extracellular vesicles (EVs) in blood plasma. We isolated medium‐sized nascent EVs of THP1 cells as well as of Optiprep‐purified platelets, and incubated them in EV‐depleted blood plasma from healthy subjects and from patients with rheumatoid arthritis. EVs were subjected to differential centrifugation, size exclusion chromatography, or density gradient ultracentrifugation followed by mass spectrometry. Plasma protein‐coated EVs had a higher density compared to the nascent ones and carried numerous newly associated proteins. Interactions between plasma proteins and EVs were confirmed by confocal microscopy, capillary Western immunoassay, immune electron microscopy and flow cytometry. We identified nine shared EV corona proteins (ApoA1, ApoB, ApoC3, ApoE, complement factors 3 and 4B, fibrinogen α‐chain, immunoglobulin heavy constant γ2 and γ4 chains), which appear to be common corona proteins among EVs, viruses and artificial nanoparticles in blood plasma. An unexpected finding of this study was the high overlap of the composition of the protein corona with blood plasma protein aggregates. This is explained by our finding that besides a diffuse, patchy protein corona, large protein aggregates also associate with the surface of EVs. However, while EVs with an external plasma protein cargo induced an increased expression of TNF‐α, IL‐6, CD83, CD86 and HLA‐DR of human monocyte‐derived dendritic cells, EV‐free protein aggregates had no effect. In conclusion, our data may shed new light on the origin of the commonly reported plasma protein ‘contamination’ of EV preparations and may add a new perspective to EV research.</abstractText>
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      <label>Tóth Eszter Ágnes et al. Formation of a protein corona on the surface of extracellular vesicles in blood plasma. (2021) JOURNAL OF EXTRACELLULAR VESICLES 2001-3078 10 11</label><template>&lt;div class=&quot;JournalArticle Publication short-list&quot;&gt; &lt;div class=&quot;authors&quot;&gt; &lt;span class=&quot;author-name&quot; mtid=&quot;10063768&quot;&gt; &lt;a href=&quot;/gui2/?type=authors&amp;mode=browse&amp;sel=10063768&quot; target=&quot;_blank&quot;&gt;Tóth, Eszter Ágnes&lt;/a&gt; &lt;/span&gt; &lt;span class=&quot;author-type&quot;&gt; &lt;/span&gt; ; &lt;span class=&quot;author-name&quot; mtid=&quot;10024570&quot;&gt; &lt;a href=&quot;/gui2/?type=authors&amp;mode=browse&amp;sel=10024570&quot; target=&quot;_blank&quot;&gt;Turiák, Lilla&lt;/a&gt; &lt;/span&gt; &lt;span class=&quot;author-type&quot;&gt; &lt;/span&gt; ; &lt;span class=&quot;author-name&quot; mtid=&quot;10026962&quot;&gt; &lt;a href=&quot;/gui2/?type=authors&amp;mode=browse&amp;sel=10026962&quot; target=&quot;_blank&quot;&gt;Visnovitz, Tamás&lt;/a&gt; &lt;/span&gt; &lt;span class=&quot;author-type&quot;&gt; &lt;/span&gt; ; &lt;span class=&quot;author-name&quot; mtid=&quot;172&quot;&gt; &lt;a href=&quot;/gui2/?type=authors&amp;mode=browse&amp;sel=172&quot; target=&quot;_blank&quot;&gt;Cserép, Csaba&lt;/a&gt; &lt;/span&gt; &lt;span class=&quot;author-type&quot;&gt; &lt;/span&gt; ; &lt;span class=&quot;author-name&quot; mtid=&quot;10055612&quot;&gt; &lt;a href=&quot;/gui2/?type=authors&amp;mode=browse&amp;sel=10055612&quot; target=&quot;_blank&quot;&gt;Mázló, Anett&lt;/a&gt; &lt;/span&gt; &lt;span class=&quot;author-type&quot;&gt; &lt;/span&gt; ; &lt;span class=&quot;author-name&quot; mtid=&quot;10043376&quot;&gt; &lt;a href=&quot;/gui2/?type=authors&amp;mode=browse&amp;sel=10043376&quot; target=&quot;_blank&quot;&gt;W. 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							&lt;span class=&quot;author-name&quot; mtid=&quot;10009113&quot;&gt;&lt;a 
																				   href=&quot;/gui2/?type=authors&amp;mode=browse&amp;sel=10009113&quot; target=&quot;_blank&quot;&gt;Kittel Ágnes
	            (&lt;span class=&quot;authorship-author-name&quot;&gt;Kittel Ágnes&lt;/span&gt;
	            &lt;span class=&quot;authorAux-mtmt&quot;&gt; Sejtbiológia&lt;/span&gt;)
			&lt;/a&gt;
    &lt;/span&gt;
&lt;span class=&quot;author-affil&quot;&gt;Institute of Experimental Medicine&lt;/span&gt;
;&amp;nbsp;&amp;nbsp;&amp;nbsp;
							&lt;span class=&quot;author-name&quot; mtid=&quot;10025749&quot;&gt;&lt;a 
																				   href=&quot;/gui2/?type=authors&amp;mode=browse&amp;sel=10025749&quot; target=&quot;_blank&quot;&gt;Nagy György
	            (&lt;span class=&quot;authorship-author-name&quot;&gt;Nagy György&lt;/span&gt;
	            &lt;span class=&quot;authorAux-mtmt&quot;&gt; Immunológia&lt;/span&gt;)
			&lt;/a&gt;
    &lt;/span&gt;
&lt;span class=&quot;author-affil&quot;&gt;&lt;span title=&quot;Semmelweis University&quot;&gt;SU&lt;/span&gt;/&lt;span title=&quot;Faculty of Medicine&quot;&gt;FM&lt;/span&gt;/&lt;span title=&quot;Instituties&quot;&gt;I&lt;/span&gt;/Department of Genetics, Cell- and Immunology; &lt;span title=&quot;Semmelweis University&quot;&gt;SU&lt;/span&gt;/&lt;span title=&quot;Faculty of Medicine&quot;&gt;FM&lt;/span&gt;/&lt;span title=&quot;Clinical&quot;&gt;C&lt;/span&gt;/&lt;span title=&quot;Department of Internal Medicine and Oncology&quot;&gt;DIMO&lt;/span&gt;/Department of Rheumatology and Clinical Immunology&lt;/span&gt;
;&amp;nbsp;&amp;nbsp;&amp;nbsp;
							&lt;span class=&quot;author-name&quot; mtid=&quot;10001933&quot;&gt;&lt;a 
																				   href=&quot;/gui2/?type=authors&amp;mode=browse&amp;sel=10001933&quot; target=&quot;_blank&quot;&gt;Bácsi Attila
	            (&lt;span class=&quot;authorship-author-name&quot;&gt;Bácsi Attila&lt;/span&gt;
	            &lt;span class=&quot;authorAux-mtmt&quot;&gt; Immunológia, molekuláris biológia&lt;/span&gt;)
			&lt;/a&gt;
    &lt;/span&gt;
&lt;span class=&quot;author-affil&quot;&gt;&lt;span title=&quot;University of Debrecen&quot;&gt;UD&lt;/span&gt;/&lt;span title=&quot;Faculty of Medicine&quot;&gt;Faculty of Medicine&lt;/span&gt;/Department of Immunology&lt;/span&gt;
;&amp;nbsp;&amp;nbsp;&amp;nbsp;
							&lt;span class=&quot;author-name&quot; mtid=&quot;10012934&quot;&gt;&lt;a 
																				   href=&quot;/gui2/?type=authors&amp;mode=browse&amp;sel=10012934&quot; target=&quot;_blank&quot;&gt;Dénes Ádám
	            (&lt;span class=&quot;authorship-author-name&quot;&gt;Dénes Ádám&lt;/span&gt;
	            &lt;span class=&quot;authorAux-mtmt&quot;&gt; Neurobiológia, neuroimmunológia&lt;/span&gt;)
			&lt;/a&gt;
    &lt;/span&gt;
&lt;span class=&quot;author-affil&quot;&gt;&lt;span title=&quot;Institute of Experimental Medicine&quot;&gt;IEM&lt;/span&gt;/Laboratory of Neuroimmunology&lt;/span&gt;
;&amp;nbsp;&amp;nbsp;&amp;nbsp;
							&lt;span class=&quot;author-name&quot; &gt;Song Gho Yong
    &lt;/span&gt;
;&amp;nbsp;&amp;nbsp;&amp;nbsp;
							&lt;span class=&quot;author-name&quot; mtid=&quot;10027645&quot;&gt;&lt;a 
																				   href=&quot;/gui2/?type=authors&amp;mode=browse&amp;sel=10027645&quot; target=&quot;_blank&quot;&gt;Szabó-Taylor Katalin É&lt;sup&gt;**&lt;/sup&gt;
	            (&lt;span class=&quot;authorship-author-name&quot;&gt;Szabó-Taylor Katalin&lt;/span&gt;
	            &lt;span class=&quot;authorAux-mtmt&quot;&gt; Immunológia&lt;/span&gt;)
			&lt;/a&gt;
    &lt;/span&gt;
&lt;span class=&quot;author-affil&quot;&gt;&lt;span title=&quot;Semmelweis University&quot;&gt;SU&lt;/span&gt;/&lt;span title=&quot;Faculty of Medicine&quot;&gt;FM&lt;/span&gt;/&lt;span title=&quot;Instituties&quot;&gt;I&lt;/span&gt;/Department of Genetics, Cell- and Immunology&lt;/span&gt;
;&amp;nbsp;&amp;nbsp;&amp;nbsp;
							&lt;span class=&quot;author-name&quot; mtid=&quot;10015596&quot;&gt;&lt;a 
																				   href=&quot;/gui2/?type=authors&amp;mode=browse&amp;sel=10015596&quot; target=&quot;_blank&quot;&gt;Buzás Edit I ✉
	            (&lt;span class=&quot;authorship-author-name&quot;&gt;Buzás Edit Irén&lt;/span&gt;
	            &lt;span class=&quot;authorAux-mtmt&quot;&gt; Immunbiológia&lt;/span&gt;)
			&lt;/a&gt;
    &lt;/span&gt;
&lt;span class=&quot;author-affil&quot;&gt;&lt;span title=&quot;Semmelweis University&quot;&gt;SU&lt;/span&gt;/&lt;span title=&quot;Faculty of Medicine&quot;&gt;FM&lt;/span&gt;/&lt;span title=&quot;Instituties&quot;&gt;I&lt;/span&gt;/Department of Genetics, Cell- and Immunology; &lt;span title=&quot;Semmelweis University&quot;&gt;SU&lt;/span&gt;/&lt;span title=&quot;Faculty of Medicine&quot;&gt;FM&lt;/span&gt;/&lt;span title=&quot;Instituties&quot;&gt;I&lt;/span&gt;/&lt;span title=&quot;Department of Genetics, Cell- and Immunology&quot;&gt;DGCI&lt;/span&gt;/MTA-SE Immune-Proteogenomics Extracellular Vesicle Research Group; &lt;span title=&quot;Semmelweis University&quot;&gt;SU&lt;/span&gt;/&lt;span title=&quot;Faculty of Medicine&quot;&gt;FM&lt;/span&gt;/&lt;span title=&quot;Instituties&quot;&gt;I&lt;/span&gt;/&lt;span title=&quot;Department of Genetics, Cell- and Immunology&quot;&gt;DGCI&lt;/span&gt;/HCEMM-SU Extracellular Vesicles Research Group&lt;/span&gt;

				    &lt;/div&gt;
&lt;/div&gt;
&lt;div class=&quot;title&quot;&gt;&lt;a href=&quot;/gui2/?mode=browse&amp;params=publication;32189675&quot; target=&quot;_blank&quot;&gt;Formation of a protein corona on the surface of extracellular vesicles in blood plasma&lt;/a&gt;&lt;/div&gt;    &lt;div&gt;		&lt;span class=&quot;journal-title&quot;&gt;JOURNAL OF EXTRACELLULAR VESICLES&lt;/span&gt;

        &lt;span class=&quot;journal-issn&quot;&gt;( &lt;a target=&quot;_blank&quot; href=&quot;https://portal.issn.org/resource/ISSN/2001-3078&quot;&gt;2001-3078&lt;/a&gt;)&lt;/span&gt;:
		&lt;span class=&quot;journal-volume&quot;&gt;10&lt;/span&gt; &lt;span class=&quot;journal-issue&quot;&gt;11&lt;/span&gt;
&lt;span class=&quot;page&quot;&gt;
		Paper e12140.
	 26 p. 
&lt;/span&gt;		 &lt;span class=&quot;year&quot;&gt;(2021)&lt;/span&gt;  
    &lt;/div&gt;
&lt;div class=&quot;pub-footer&quot;&gt;
    

	&lt;span class=&quot;language&quot; xmlns=&quot;http://www.w3.org/1999/html&quot;&gt;Language:
			English
		 |  &lt;/span&gt;

	&lt;span class=&quot;identifiers&quot;&gt;
						&lt;span class=&quot;id identifier oa_GOLD&quot; title=&quot;	Gold
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							&lt;a style=&quot;color:blue&quot; title=&quot;10.1002/jev2.12140&quot; target=&quot;_blank&quot; href=&quot;https://doi.org/10.1002/jev2.12140&quot;&gt;
									DOI
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						&lt;span class=&quot;id identifier oa_GREEN&quot; title=&quot;	Green
&quot;&gt;
							
							&lt;a style=&quot;color:black&quot; title=&quot;129756&quot; target=&quot;_blank&quot; href=&quot;https://real.mtak.hu/129756&quot;&gt;
									REAL
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						&lt;span class=&quot;id identifier oa_none&quot; title=&quot;none&quot;&gt;
							
							&lt;a style=&quot;color:blue&quot; title=&quot;000695648800001&quot; target=&quot;_blank&quot; href=&quot;https://www.webofscience.com/wos/woscc/full-record/000695648800001&quot;&gt;
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						&lt;span class=&quot;id identifier oa_none&quot; title=&quot;none&quot;&gt;
							
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									PubMed
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	&lt;OnlyViewableByAuthor&gt;&lt;div class=&quot;ratings&quot;&gt;
				&lt;div class=&quot;journal-subject&quot;&gt;Journal subject: Scopus - Cell Biology&amp;nbsp;&amp;nbsp;&amp;nbsp;Rank:&amp;nbsp;D1&lt;/div&gt;
				&lt;div class=&quot;journal-subject&quot;&gt;Journal subject: Scopus - Histology&amp;nbsp;&amp;nbsp;&amp;nbsp;Rank:&amp;nbsp;D1&lt;/div&gt;
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	&lt;div class=&quot;publication-citation&quot; style=&quot;margin-left: 0.5cm;&quot;&gt;
		&lt;span title=&quot;&quot; class=&quot;citingPub-count&quot;&gt;Citing papers: 468&lt;/span&gt;
		| Independent citation: 449
		| Self citation: 19
		| Unknown citation: 0
		| Number of citations in WoS: 444 
		|  Number of citations in Scopus:&amp;nbsp;438 
		|  WoS/Scopus assigned:&amp;nbsp;459 
		|  Number of citations with DOI:&amp;nbsp;464 
		
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	&lt;div class=&quot;publication-citation&quot;&gt;
		&lt;a target=&quot;_blank&quot; href=&quot;/api/publication?cond=citations.related;eq;32189675&amp;sort=publishedYear,desc&amp;sort=title&quot;&gt;
			Number of cited publications: 23
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    &lt;div class=&quot;mtid&quot;&gt;&lt;span class=&quot;long-pub-mtid&quot;&gt;Publication: 32189675&lt;/span&gt;
    | &lt;span class=&quot;status-data status-VALIDATED&quot;&gt; 	Validated
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Core	 Citing
	
	
    | &lt;span class=&quot;type-subtype&quot;&gt;Journal Article
			( Article
			
			)
		&lt;/span&gt;
      		| &lt;span class=&quot;pub-category&quot;&gt;Scientific&lt;/span&gt;
	| &lt;span class=&quot;publication-sourceOfData&quot;&gt;kézi felvitel&lt;/span&gt;
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&lt;div class=&quot;funder&quot;&gt; (K 125337) Funder: NRDIO,    Hungarian Scientific Research Fund(11958) Funder: HSRF,    (120237) Funder: HSRF,    (OTKA K131479) Funder: HSRF,    (PD121187) Funder: HSRF,    (OTKA FK 131603) Funder: HSRF,    (OTKA 131762) Funder: HSRF,    National Heart Program(NVKP-16-1-2016-0017) Funder: NRDIO,    (ÚNKP-19-3-I-SE-45),    (UNKP-20-5),    (VEKOP-2.3.2-16-2016-00002) Funder: NRDIO,    (VEKOP-2.3.3.-15-2017-00016),    Az orvos-, egészségtudományi- és gyógyszerészképzés tudományos műhelyeinek fejlesztése(EFOP-3.6.3-VEKOP-16-2017-00009) Funder: EFOP-VEKOP,    (LP2016-4/2016) Funder: MTA   &lt;/div&gt;
&lt;div class=&quot;lastModified&quot;&gt;Last Modified: 2026.02.11. 14:48 András Balázs (MTMT Központ, admin)
&lt;/div&gt;


        &lt;div class=&quot;lockedBy&quot;&gt;Centrally managed 2026.01.21. 14:12  Katalin Mikola (MTMT 3, admin)
        &lt;/div&gt;



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