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      <abstractText>In this study we tested whether a protein corona is formed around extracellular vesicles (EVs) in blood plasma. We isolated medium‐sized nascent EVs of THP1 cells as well as of Optiprep‐purified platelets, and incubated them in EV‐depleted blood plasma from healthy subjects and from patients with rheumatoid arthritis. EVs were subjected to differential centrifugation, size exclusion chromatography, or density gradient ultracentrifugation followed by mass spectrometry. Plasma protein‐coated EVs had a higher density compared to the nascent ones and carried numerous newly associated proteins. Interactions between plasma proteins and EVs were confirmed by confocal microscopy, capillary Western immunoassay, immune electron microscopy and flow cytometry. We identified nine shared EV corona proteins (ApoA1, ApoB, ApoC3, ApoE, complement factors 3 and 4B, fibrinogen α‐chain, immunoglobulin heavy constant γ2 and γ4 chains), which appear to be common corona proteins among EVs, viruses and artificial nanoparticles in blood plasma. An unexpected finding of this study was the high overlap of the composition of the protein corona with blood plasma protein aggregates. This is explained by our finding that besides a diffuse, patchy protein corona, large protein aggregates also associate with the surface of EVs. However, while EVs with an external plasma protein cargo induced an increased expression of TNF‐α, IL‐6, CD83, CD86 and HLA‐DR of human monocyte‐derived dendritic cells, EV‐free protein aggregates had no effect. In conclusion, our data may shed new light on the origin of the commonly reported plasma protein ‘contamination’ of EV preparations and may add a new perspective to EV research.</abstractText>
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&lt;span class=&quot;author-affil&quot;&gt;&lt;span title=&quot;Semmelweis Egyetem&quot;&gt;SE&lt;/span&gt;/&lt;span title=&quot;Általános Orvostudományi Kar&quot;&gt;AOK&lt;/span&gt;/&lt;span title=&quot;Intézet&quot;&gt;I&lt;/span&gt;/I. Sz. Patológiai és Kísérleti Rákkutató Intézet&lt;/span&gt;
;&amp;nbsp;&amp;nbsp;&amp;nbsp;
							&lt;span class=&quot;author-name&quot; mtid=&quot;10026923&quot;&gt;&lt;a 
																				   href=&quot;/gui2/?type=authors&amp;mode=browse&amp;sel=10026923&quot; target=&quot;_blank&quot;&gt;Komlósi Zsolt
            (&lt;span class=&quot;authorship-author-name&quot;&gt;Komlósi Zsolt&lt;/span&gt;
            &lt;span class=&quot;authorAux-mtmt&quot;&gt; Pulmonológia&lt;/span&gt;)
			&lt;/a&gt;
    &lt;/span&gt;
&lt;span class=&quot;author-affil&quot;&gt;&lt;span title=&quot;Semmelweis Egyetem&quot;&gt;SE&lt;/span&gt;/&lt;span title=&quot;Általános Orvostudományi Kar&quot;&gt;AOK&lt;/span&gt;/&lt;span title=&quot;Intézet&quot;&gt;I&lt;/span&gt;/Genetikai, Sejt- és Immunbiológiai Intézet&lt;/span&gt;
;&amp;nbsp;&amp;nbsp;&amp;nbsp;
							&lt;span class=&quot;author-name&quot; mtid=&quot;10002323&quot;&gt;&lt;a 
																				   href=&quot;/gui2/?type=authors&amp;mode=browse&amp;sel=10002323&quot; target=&quot;_blank&quot;&gt;Drahos László
            (&lt;span class=&quot;authorship-author-name&quot;&gt;Drahos László&lt;/span&gt;
            &lt;span class=&quot;authorAux-mtmt&quot;&gt; proteomika, tömegspektrometria&lt;/span&gt;)
			&lt;/a&gt;
    &lt;/span&gt;
&lt;span class=&quot;author-affil&quot;&gt;&lt;span title=&quot;MTA Természettudományi Kutatóközpont&quot;&gt;MTA TTK&lt;/span&gt;/Szerves Kémiai Intézet&lt;/span&gt;
;&amp;nbsp;&amp;nbsp;&amp;nbsp;
							&lt;span class=&quot;author-name&quot; mtid=&quot;10009113&quot;&gt;&lt;a 
																				   href=&quot;/gui2/?type=authors&amp;mode=browse&amp;sel=10009113&quot; target=&quot;_blank&quot;&gt;Kittel Ágnes
            (&lt;span class=&quot;authorship-author-name&quot;&gt;Kittel Ágnes&lt;/span&gt;
            &lt;span class=&quot;authorAux-mtmt&quot;&gt; Sejtbiológia&lt;/span&gt;)
			&lt;/a&gt;
    &lt;/span&gt;
&lt;span class=&quot;author-affil&quot;&gt;Kísérleti Orvostudományi Kutatóintézet&lt;/span&gt;
;&amp;nbsp;&amp;nbsp;&amp;nbsp;
							&lt;span class=&quot;author-name&quot; mtid=&quot;10025749&quot;&gt;&lt;a 
																				   href=&quot;/gui2/?type=authors&amp;mode=browse&amp;sel=10025749&quot; target=&quot;_blank&quot;&gt;Nagy György
            (&lt;span class=&quot;authorship-author-name&quot;&gt;Nagy György&lt;/span&gt;
            &lt;span class=&quot;authorAux-mtmt&quot;&gt; Immunológia&lt;/span&gt;)
			&lt;/a&gt;
    &lt;/span&gt;
&lt;span class=&quot;author-affil&quot;&gt;&lt;span title=&quot;Semmelweis Egyetem&quot;&gt;SE&lt;/span&gt;/&lt;span title=&quot;Általános Orvostudományi Kar&quot;&gt;AOK&lt;/span&gt;/&lt;span title=&quot;Intézet&quot;&gt;I&lt;/span&gt;/Genetikai, Sejt- és Immunbiológiai Intézet; &lt;span title=&quot;Semmelweis Egyetem&quot;&gt;SE&lt;/span&gt;/&lt;span title=&quot;Általános Orvostudományi Kar&quot;&gt;AOK&lt;/span&gt;/&lt;span title=&quot;Klinikum&quot;&gt;K&lt;/span&gt;/&lt;span title=&quot;Belgyógyászati és Onkológiai Klinika&quot;&gt;BOK&lt;/span&gt;/Reumatológiai és Klinikai Immunológiai Tanszék&lt;/span&gt;
;&amp;nbsp;&amp;nbsp;&amp;nbsp;
							&lt;span class=&quot;author-name&quot; mtid=&quot;10001933&quot;&gt;&lt;a 
																				   href=&quot;/gui2/?type=authors&amp;mode=browse&amp;sel=10001933&quot; target=&quot;_blank&quot;&gt;Bácsi Attila
            (&lt;span class=&quot;authorship-author-name&quot;&gt;Bácsi Attila&lt;/span&gt;
            &lt;span class=&quot;authorAux-mtmt&quot;&gt; Immunológia, molekuláris biológia&lt;/span&gt;)
			&lt;/a&gt;
    &lt;/span&gt;
&lt;span class=&quot;author-affil&quot;&gt;&lt;span title=&quot;Debreceni Egyetem&quot;&gt;DE&lt;/span&gt;/&lt;span title=&quot;Általános Orvostudományi Kar&quot;&gt;ÁOK&lt;/span&gt;/Immunológiai Intézet&lt;/span&gt;
;&amp;nbsp;&amp;nbsp;&amp;nbsp;
							&lt;span class=&quot;author-name&quot; mtid=&quot;10012934&quot;&gt;&lt;a 
																				   href=&quot;/gui2/?type=authors&amp;mode=browse&amp;sel=10012934&quot; target=&quot;_blank&quot;&gt;Dénes Ádám
            (&lt;span class=&quot;authorship-author-name&quot;&gt;Dénes Ádám&lt;/span&gt;
            &lt;span class=&quot;authorAux-mtmt&quot;&gt; Neurobiológia, neuroimmunológia&lt;/span&gt;)
			&lt;/a&gt;
    &lt;/span&gt;
&lt;span class=&quot;author-affil&quot;&gt;&lt;span title=&quot;Kísérleti Orvostudományi Kutatóintézet&quot;&gt;KOKI&lt;/span&gt;/Neuroimmunológiai Kutatócsoport&lt;/span&gt;
;&amp;nbsp;&amp;nbsp;&amp;nbsp;
							&lt;span class=&quot;author-name&quot; &gt;Song Gho Yong
    &lt;/span&gt;
;&amp;nbsp;&amp;nbsp;&amp;nbsp;
							&lt;span class=&quot;author-name&quot; mtid=&quot;10027645&quot;&gt;&lt;a 
																				   href=&quot;/gui2/?type=authors&amp;mode=browse&amp;sel=10027645&quot; target=&quot;_blank&quot;&gt;Szabó-Taylor Katalin É&lt;sup&gt;**&lt;/sup&gt;
            (&lt;span class=&quot;authorship-author-name&quot;&gt;Szabó-Taylor Katalin&lt;/span&gt;
            &lt;span class=&quot;authorAux-mtmt&quot;&gt; Immunológia&lt;/span&gt;)
			&lt;/a&gt;
    &lt;/span&gt;
&lt;span class=&quot;author-affil&quot;&gt;&lt;span title=&quot;Semmelweis Egyetem&quot;&gt;SE&lt;/span&gt;/&lt;span title=&quot;Általános Orvostudományi Kar&quot;&gt;AOK&lt;/span&gt;/&lt;span title=&quot;Intézet&quot;&gt;I&lt;/span&gt;/Genetikai, Sejt- és Immunbiológiai Intézet&lt;/span&gt;
;&amp;nbsp;&amp;nbsp;&amp;nbsp;
							&lt;span class=&quot;author-name&quot; mtid=&quot;10015596&quot;&gt;&lt;a 
																				   href=&quot;/gui2/?type=authors&amp;mode=browse&amp;sel=10015596&quot; target=&quot;_blank&quot;&gt;Buzás Edit I ✉
            (&lt;span class=&quot;authorship-author-name&quot;&gt;Buzás Edit Irén&lt;/span&gt;
            &lt;span class=&quot;authorAux-mtmt&quot;&gt; Immunbiológia&lt;/span&gt;)
			&lt;/a&gt;
    &lt;/span&gt;
&lt;span class=&quot;author-affil&quot;&gt;&lt;span title=&quot;Semmelweis Egyetem&quot;&gt;SE&lt;/span&gt;/&lt;span title=&quot;Általános Orvostudományi Kar&quot;&gt;AOK&lt;/span&gt;/&lt;span title=&quot;Intézet&quot;&gt;I&lt;/span&gt;/Genetikai, Sejt- és Immunbiológiai Intézet; &lt;span title=&quot;Semmelweis Egyetem&quot;&gt;SE&lt;/span&gt;/&lt;span title=&quot;Általános Orvostudományi Kar&quot;&gt;AOK&lt;/span&gt;/&lt;span title=&quot;Intézet&quot;&gt;I&lt;/span&gt;/&lt;span title=&quot;Genetikai, Sejt- és Immunbiológiai Intézet&quot;&gt;GSII&lt;/span&gt;/MTA-SE Immun-proteogenomikai Extracelluláris Vezikula Kutatócsoport; &lt;span title=&quot;Semmelweis Egyetem&quot;&gt;SE&lt;/span&gt;/&lt;span title=&quot;Általános Orvostudományi Kar&quot;&gt;AOK&lt;/span&gt;/&lt;span title=&quot;Intézet&quot;&gt;I&lt;/span&gt;/&lt;span title=&quot;Genetikai, Sejt- és Immunbiológiai Intézet&quot;&gt;GSII&lt;/span&gt;/HCEMM-SE Extracelluláris Vezikula Kutatócsoport&lt;/span&gt;

				    &lt;/div&gt;
&lt;/div&gt;
&lt;div class=&quot;title&quot;&gt;&lt;a href=&quot;/gui2/?mode=browse&amp;params=publication;32189675&quot; target=&quot;_blank&quot;&gt;Formation of a protein corona on the surface of extracellular vesicles in blood plasma&lt;/a&gt;&lt;/div&gt;    &lt;div&gt;		&lt;span class=&quot;journal-title&quot;&gt;JOURNAL OF EXTRACELLULAR VESICLES&lt;/span&gt;

        &lt;span class=&quot;journal-issn&quot;&gt;( &lt;a target=&quot;_blank&quot; href=&quot;https://portal.issn.org/resource/ISSN/2001-3078&quot;&gt;2001-3078&lt;/a&gt;)&lt;/span&gt;:
		&lt;span class=&quot;journal-volume&quot;&gt;10&lt;/span&gt; &lt;span class=&quot;journal-issue&quot;&gt;11&lt;/span&gt;
&lt;span class=&quot;page&quot;&gt;
		Paper e12140.
	 26 p. 
&lt;/span&gt;		 &lt;span class=&quot;year&quot;&gt;(2021)&lt;/span&gt;  
    &lt;/div&gt;
&lt;div class=&quot;pub-footer&quot;&gt;
    

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									DOI
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				&lt;div class=&quot;journal-subject&quot;&gt;Folyóirat szakterülete: Scopus - Cell Biology&amp;nbsp;&amp;nbsp;&amp;nbsp;SJR indikátor:&amp;nbsp;D1&lt;/div&gt;
				&lt;div class=&quot;journal-subject&quot;&gt;Folyóirat szakterülete: Scopus - Histology&amp;nbsp;&amp;nbsp;&amp;nbsp;SJR indikátor:&amp;nbsp;D1&lt;/div&gt;
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		&lt;span title=&quot;Nyilvános idézőközlemények összesen, említések nélkül&quot; class=&quot;citingPub-count&quot;&gt;Nyilvános idéző összesen: 400&lt;/span&gt;
		| Független: 382
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		| WoS jelölt: 378 
		|  Scopus jelölt:&amp;nbsp;371 
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		|  DOI jelölt:&amp;nbsp;394 
		
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    &lt;div class=&quot;mtid&quot;&gt;&lt;span class=&quot;long-pub-mtid&quot;&gt;Közlemény: 32189675&lt;/span&gt;
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    | &lt;span class=&quot;type-subtype&quot;&gt;Folyóiratcikk
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		&lt;/span&gt;
      		| &lt;span class=&quot;pub-category&quot;&gt;Tudományos&lt;/span&gt;
	| &lt;span class=&quot;publication-sourceOfData&quot;&gt;kézi felvitel&lt;/span&gt;
&lt;/div&gt;

&lt;div class=&quot;funder&quot;&gt; (K 125337) Támogató: NKFIH,    Hungarian Scientific Research Fund(11958) Támogató: OTKA,    (120237) Támogató: OTKA,    (OTKA K131479) Támogató: OTKA,    (PD121187) Támogató: OTKA,    (OTKA FK 131603) Támogató: OTKA,    (OTKA 131762) Támogató: OTKA,    National Heart Program(NVKP-16-1-2016-0017) Támogató: NKFIH,    (ÚNKP-19-3-I-SE-45),    (UNKP-20-5),    (VEKOP-2.3.2-16-2016-00002) Támogató: NKFIH,    (VEKOP-2.3.3.-15-2017-00016),    Az orvos-, egészségtudományi- és gyógyszerészképzés tudományos műhelyeinek fejlesztése(EFOP-3.6.3-VEKOP-16-2017-00009) Támogató: EFOP-VEKOP,    (LP2016-4/2016) Támogató: MTA   &lt;/div&gt;
&lt;div class=&quot;lastModified&quot;&gt;Utolsó módosítás: 2026.02.11. 14:48 Balázs András (MTMT Központ, admin)
&lt;/div&gt;


        &lt;div class=&quot;lockedBy&quot;&gt;Központi kezelésű 2026.01.21. 14:12  Mikola Katalin (MTMT 3, admin)
        &lt;/div&gt;



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