DNA synthesis, modification, repair, recombination and degradation
MEDICAL AND HEALTH SCIENCES
UV-induced DNA damage response and repair are extensively studied processes, as any
malfunction in these pathways contributes to the activation of tumorigenesis. Although
several proteins involved in these cellular mechanisms have been described, the entire
repair cascade has remained unexplored. To identify new players in UV-induced repair,
we performed a microarray screen, in which we found SerpinB10 (SPB10, Bomapin) as
one of the most dramatically upregulated genes following UV irradiation. Here, we
demonstrated that an increased mRNA level of SPB10 is a general cellular response
following UV irradiation regardless of the cell type. We showed that although SPB10
is implicated in the UV-induced cellular response, it has no indispensable function
in cell survival upon UV irradiation. Nonetheless, we revealed that SPB10 might be
involved in delaying the duration of DNA repair in interphase and also in S-phase
cells. Additionally, we also highlighted the interaction between SPB10 and H3. Based
on our results, it seems that SPB10 protein is implicated in UV-induced stress as
a “quality control protein”, presumably by slowing down the repair process.
