Insecticidal and Antiprotozoal Properties of Lichen Secondary Metabolites on Insect Vectors and Their Transmitted Protozoal Diseases to Humans

https://m2.mtmt.hu/ eng 2026-04-16 21:37 JournalArticle 32164754 APPROVED true Export Date: 31 August 2021 Correspondence Address: Farkas, E.É.; Institute of Ecology and Botany, Alkotmány u. 2–4, Hungary; email: farkas.edit@ecolres.hu 0 false 2025-12-31T14:46:05.360+0000 2025-02-25T22:57:42.054+0000 2021-08-30T10:55:29.745+0000 true 10017064 Ribai Richárdné /api/admin/10017064 Admin Richárdné Ribai (ÖK ÖBI admin 5) true 10017064 2025-02-25T22:57:42.263+0000 2025-02-25T22:57:42.263+0000 true false true 24 24 /api/publicationtype/24 PublicationType Journal Article 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType Journal Article 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType Article (Journal Article) 101 true 10000059 true 1 /api/category/1 Category Scientific true 1 Muhoro, Arthur M. Insecticidal and Antiprotozoal Properties of Lichen Secondary Metabolites on Insect Vectors and Their Transmitted Protozoal Diseases to Humans true true /api/journal/10027240 REVIEWED DIVERSITY (BASEL) 1424-2818 true false 10027240 false 10027240 true 1424-2818 Journal FOREIGN 13 8 342 342 2021 Since the long-term application of synthetic chemicals as insecticides and the chemotherapy of protozoal diseases have had various negative effects (non-target effects, resistance), research on less harmful biological products is underway. This review is focused on lichens with potential insecticidal and antiprotozoal activity. Literature sources (27) were surveyed from five bibliographic databases and analyzed according to the taxonomic group of the insect, the protozoal disease and the lichen, the type of bioactive compounds (including method of application and mount applied), and the potential bioactivity based on mortalities caused after 24 h of exposure on insects and on parasitic protozoa. Six species of protozoa and five species of mosquitoes, three kinds of larval stages of insects and three protozoa stages were tested. Insecticidal and antiprotozoal effects of crude extracts and seven lichen secondary metabolites (mostly usnic acid) of 32 lichen species were determined. Physiological and morphological changes on parasitic protozoa were observed. Mortality rates caused by LSMs on insect vectors closer to (or somewhat above) the WHO threshold were considered to be insecticides. The results are based on laboratory experiments; however, the efficacy of metabolites should be confirmed in the field and on non-human primates to control the insect vectors and human protozoal diseases transmitted by insects. true 2021 true true true false true false GOLD 2025-12-31 true https://doi.org/10.3390/d13080342 9 0 9 3 3 8 9 8 8 9 9 3 3 3 6 0 9 3 9 3 0 2 2 Folyóiratcikk Journal Article 24 9 Könyvrészlet Chapter in Book 25 0 Könyv Book 23 0 Egyéb konferenciaközlemény Conference paper 31 0 Egyéb konferenciakötet Conference proceedings 32 0 Oltalmi formák Protection forms 26 0 Disszertáció Thesis 28 0 Egyéb Miscellaneous 29 0 Alkotás Achievement 22 0 Kutatási adat Research data 33 0 Folyóiratcikk Journal Article 24 0 0 0 Könyvrészlet Chapter in Book 25 0 0 0 Könyv Book 23 0 0 0 Egyéb konferenciaközlemény Conference paper 31 0 0 0 Egyéb konferenciakötet Conference proceedings 32 0 0 0 Oltalmi formák Protection forms 26 0 0 0 Disszertáció Thesis 28 0 0 0 Egyéb Miscellaneous 29 0 0 0 Alkotás Achievement 22 0 0 0 Kutatási adat Research data 33 0 0 0 2022 0 2 0 2 0 2023 0 2 2 2 2 2024 0 4 0 4 0 2025 0 1 1 1 1 Q1 false 10003979 false false false 2021-08-29T10:55:53.359+0000 Ökológiai és Botanikai Intézet (HUN-REN ÖK) 2 9 1 2 0 true Language 10002 /api/language/10002 English Angol English true 2 true PersonAuthorship 97232810 /api/authorship/97232810 Muhoro, Arthur M. [Muhoro, Arthur (BIOLOGICAL ACTIVI...), author] 1 0.5 true false false Author 10082352 /api/author/10082352 Arthur Muhoro (BIOLOGICAL ACTIVITIES OF LICHEN SECONDARY METABOLITES) Muhoro Arthur true true Muhoro Arthur M. true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false true PersonAuthorship 97232811 /api/authorship/97232811 Farkas, Edit [Farkas, Edit (Növénytan, lichen...), author] Institute of Ecology and Botany 2 0.5 false true false Author 10003979 /api/author/10003979 Edit Farkas (Növénytan, lichenológia) Farkas Edit true 10003979 true Farkas Edit true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false true PublicationIdentifier 19182625 /api/publicationidentifier/19182625 DOI: 10.3390/d13080342 PlainSource 6 /api/publicationsource/6 DOI PublicationSourceType 10001 /api/publicationsourcetype/10001 DOI true true true DOI DOI https://doi.org/@@@ true true 6 true GOLD true IDENTICAL 10.3390/d13080342 https://doi.org/10.3390/d13080342 false true PublicationIdentifier 19733547 /api/publicationidentifier/19733547 WoS: 000688960000001 PlainSource 1 /api/publicationsource/1 WoS PublicationSourceType 10003 /api/publicationsourcetype/10003 Indexelő adatbázis false true true WoS WoS https://www.webofscience.com/wos/woscc/full-record/@@@ true true 1 true IDENTICAL 000688960000001 https://www.webofscience.com/wos/woscc/full-record/000688960000001 false true PublicationIdentifier 19182626 /api/publicationidentifier/19182626 Scopus: 85112170678 PlainSource 3 /api/publicationsource/3 Scopus PublicationSourceType 10003 /api/publicationsourcetype/10003 Indexelő adatbázis false true true Scopus Scopus http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-@@@ true true 3 true IDENTICAL 85112170678 http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85112170678 false true SjrRating 11185221 /api/sjrrating/11185221 sjr:Q1 (2021) Scopus - Agricultural and Biological Sciences (miscellaneous) DIVERSITY-BASEL 1424-2818 58 0.24 journal RatingType 10002 /api/ratingtype/10002 sjr sjr true true ClassificationExternal 1101 /api/classificationexternal/1101 Scopus - Agricultural and Biological Sciences (miscellaneous) true 1101 true Q1 DIRECT true true Reference 22567420 /api/reference/22567420 1. Yildirim 2012: Insecticidal effect of Usnea longissima (Parmeliaceae) extract against Sitophilus granarius (Coleoptera: Curculionidae)., Int. J. Agric. Biol., 14, p. 303 1 false true Reference 22567421 /api/reference/22567421 2. WHO 2020, World Health Organization/News Room/ Fact Sheets/Detail/Malaria, 1 April 2021 https://www.who.int/news-room/fact-sheets/detail/malaria 2 false true Reference 22567422 /api/reference/22567422 3. Chen 2005: Non-vector transmission of dengue and other mosquito-borne Flaviviruses., Dengue Bull., 29, p. 18 3 false true Reference 22567423 /api/reference/22567423 4. Dar 2010: Environmental changes and emerging vectorborne diseases: A review., Biol. Forum Int. J., 2, p. 78 4 false true Reference 22567424 /api/reference/22567424 5. , DOI: 10.3844/ajeassp.2011.187.200 5 10.3844/ajeassp.2011.187.200 false true Reference 22567425 /api/reference/22567425 6. , DOI: 10.1515/jppr-2015-0033 6 10.1515/jppr-2015-0033 false true Reference 22567426 /api/reference/22567426 7. , DOI: 10.1179/2047773212Y.0000000051 7 10.1179/2047773212Y.0000000051 false true Reference 22567427 /api/reference/22567427 8. Singh 2015: Mosquito borne zoonotic diseases: A review., J. Livest. Sci., 6, p. 65 8 false true Reference 22567428 /api/reference/22567428 9. , DOI: 10.1016/j.ibiod.2009.05.009 9 10.1016/j.ibiod.2009.05.009 false true Reference 22567429 /api/reference/22567429 10. , DOI: 10.1897/05-438R.1 10 10.1897/05-438R.1 false true Reference 22567430 /api/reference/22567430 11. , DOI: 10.1289/ehp.1103944 11 10.1289/ehp.1103944 false true Reference 22567431 /api/reference/22567431 12. , DOI: 10.1007/s12639-016-0812-3 12 10.1007/s12639-016-0812-3 false true Reference 22567432 /api/reference/22567432 13. , DOI: 10.1007/s00436-008-0905-8 13 10.1007/s00436-008-0905-8 false true Reference 22567433 /api/reference/22567433 14. Ghosh 2012: Plant extracts as potential mosquito larvicides., Indian J. Med. Res., 135, p. 581 14 false true Reference 22567434 /api/reference/22567434 15. Gupta 2010: Biopesticides: An ecofriendly approach for pest control., J. Biopest., 3, p. 186 15 false true Reference 22567435 /api/reference/22567435 16. , DOI: 10.4172/2155-6202.1000e107 16 10.4172/2155-6202.1000e107 false true Reference 22567436 /api/reference/22567436 17. , DOI: 10.1515/znc-2010-3-401 17 10.1515/znc-2010-3-401 false true Reference 22567437 /api/reference/22567437 18. , DOI: 10.4038/jnsfsr.v33i2.2345 18 10.4038/jnsfsr.v33i2.2345 false true Reference 22567438 /api/reference/22567438 19. , DOI: 10.1016/j.cropro.2013.10.022 19 10.1016/j.cropro.2013.10.022 false true Reference 22567439 /api/reference/22567439 20. , DOI: 10.1371/journal.pntd.0005128 20 10.1371/journal.pntd.0005128 false true Reference 22567440 /api/reference/22567440 21. , DOI: 10.16943/ptinsa/2014/v80i2/3 21 10.16943/ptinsa/2014/v80i2/3 false true Reference 22567441 /api/reference/22567441 22. Bostan 2013: Allelopathic effect of Festuca rubra on perennial grasses., Rom. Biotechnol. Lett., 18, p. 8190 22 false true Reference 22567442 /api/reference/22567442 23. , DOI: 10.1007/BF02066241 23 10.1007/BF02066241 false true Reference 22567443 /api/reference/22567443 24. Larhsini 1997: Evaluation of antifungal and molluscicidal properties of extracts of Calotropis procera., Fitoterapia, 68, p. 371 24 false true Reference 22567444 /api/reference/22567444 25. Alam 2009: Insecticidal activity of root bark of Calotropis gigantea L. against Tribolium castaneum (Herbst)., World. J. Zool., 4, p. 90 25 false true Reference 22567445 /api/reference/22567445 26. Singh 2005: Laboratory study on larvicidal properties of leaf extract of Calotropis procera (Family-Asclepiadaceae) against mosquito larvae., J. Commun. Dis., 37, p. 109 26 false true Reference 22567446 /api/reference/22567446 27. Singhi 2004: Ovipositioning behaviour of Aedes aegypti in different concentrations of latex of Calotropis procera: Studies on refractory behaviour and its sustenance across gonotrophic cycles., Dengue Bull., 28, p. 184 27 false true Reference 22567447 /api/reference/22567447 28. Moncayo 1993: Program Report of the UNPD/World Bank/WHO Special Program for Research and Training in Tropical Diseases (TDR)p. 67 28 false true Reference 22567448 /api/reference/22567448 29. , DOI: 10.4269/ajtmh.1998.59.526 29 10.4269/ajtmh.1998.59.526 false true Reference 22567449 /api/reference/22567449 30. , DOI: 10.1016/0001-706X(93)90021-3 30 10.1016/0001-706X(93)90021-3 false true Reference 22567450 /api/reference/22567450 31. , DOI: 10.3390/microorganisms9030578 31 10.3390/microorganisms9030578 false true Reference 22567451 /api/reference/22567451 32. , DOI: 10.1093/clinids/10.3.560 32 10.1093/clinids/10.3.560 false true Reference 22567452 /api/reference/22567452 33. , DOI: 10.1016/0165-6147(88)90258-1 33 10.1016/0165-6147(88)90258-1 false true Reference 22567453 /api/reference/22567453 34. , DOI: 10.7324/japs.2018.81020 34 10.7324/japs.2018.81020 false true Reference 22567454 /api/reference/22567454 35. , DOI: 10.1177/014107680309600304 35 10.1177/014107680309600304 false true Reference 22567455 /api/reference/22567455 36. , DOI: 10.1016/j.micron.2004.09.003 36 10.1016/j.micron.2004.09.003 false true Reference 22567456 /api/reference/22567456 37. , DOI: 10.1016/S0742-8413(96)00127-2 37 10.1016/S0742-8413(96)00127-2 false true Reference 22567457 /api/reference/22567457 38. , DOI: 10.1016/j.chmed.2018.03.002 38 10.1016/j.chmed.2018.03.002 false true Reference 22567458 /api/reference/22567458 39. Karthik 2011: Total phenol content, insecticidal and amylase inhibitory efficacy of Heterodermia leucomela (L)., Ann. Biol. Res., 2, p. 38 39 false true Reference 22567459 /api/reference/22567459 40. Vinayaka 2009: Larvicidal and wormicidal efficacy of methanolic extracts of five macrolichens collected from Bhadra wildlife sanctuary., Biomedicine, 29, p. 327 40 false true Reference 22567460 /api/reference/22567460 41. Kumar 2010: Insecticidal efficacy of Ramalina hossei H. Magn & G. Awasthi and Ramalina conduplicans Vain.—macrolichens from Bhadra wildlife sanctuary, Karnataka., Biomedicine, 30, p. 100 41 false true Reference 22567461 /api/reference/22567461 42. , DOI: 10.1016/j.bjp.2016.03.006 42 10.1016/j.bjp.2016.03.006 false true Reference 22567462 /api/reference/22567462 43. , DOI: 10.1021/np400083k 43 10.1021/np400083k false true Reference 22567463 /api/reference/22567463 44. , DOI: 10.1080/14786411003774296 44 10.1080/14786411003774296 false true Reference 22567464 /api/reference/22567464 45. Mosquito Taxonomic Inventory http://mosquito-taxonomic-inventory.info 45 false true Reference 22567465 /api/reference/22567465 46. 2005: Guidelines for Laboratory and Field Testing of Mosquito Larvicidesp. 1 46 false true Reference 22567466 /api/reference/22567466 47. Bomfim 2009: Larvicidal activity of Cladonia substellata extract and usnic acid against Aedes aegypti and Artemia salina., Lat. Am. J. Pharm., 28, p. 580 47 false true Reference 22567467 /api/reference/22567467 48. 2006: Guidelines for Testing Mosquito Adulticides for Indoor Residual Spraying and Treatment of Mosquito Netsp. 1 48 false true Reference 22567468 /api/reference/22567468 49. , DOI: 10.1556/034.62.2020.1-2.4 49 10.1556/034.62.2020.1-2.4 false true Reference 22567469 /api/reference/22567469 50. , DOI: 10.1016/j.exppara.2016.03.021 50 10.1016/j.exppara.2016.03.021 false true Reference 22567470 /api/reference/22567470 51. Wu 1995: Effect of Cladonia alpestris on Trichomonas vaginalis in vitro., Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi [Chin. J. Parasitol. Parasit. Dis.], 13, p. 126 51 false true Reference 22567471 /api/reference/22567471 52. , DOI: 10.1016/j.bcp.2003.09.032 52 10.1016/j.bcp.2003.09.032 false true Reference 22567472 /api/reference/22567472 53. , DOI: 10.1016/j.jep.2003.10.019 53 10.1016/j.jep.2003.10.019 false true Reference 22567473 /api/reference/22567473 54. Kinoshita 1997: Distribution of optical isomers of usnic and isousnic acids analyzed by high performance liquid chromatography., J. Hattori Bot. Lab., 83, p. 173 54 false true Reference 22567474 /api/reference/22567474 55. , DOI: 10.1007/s11101-019-09605-3 55 10.1007/s11101-019-09605-3 false true Reference 22567475 /api/reference/22567475 56. Kirk 2008: Ainsworth & Bisby’s Dictionary of the Fungip. 1 56 false true Reference 22567476 /api/reference/22567476 57. , DOI: 10.1039/B606983P 57 10.1039/B606983P false true Reference 22567477 /api/reference/22567477 58. Samfira 2015: Characterization and identity confirmation of essential oils by mid infrared absorption spectrophotometry., Dig. J. Nanomat. Biostruct., 10, p. 557 58 false true Reference 22567478 /api/reference/22567478 59. , DOI: 10.2298/JSC101004087S 59 10.2298/JSC101004087S false true Reference 22567479 /api/reference/22567479 60. Barbat 2013: Microencapsulation of the allelochemical compounds and study of their release from different products., Dig. J. Nanomat. Biostruct., 8, p. 945 60 false true /api/publication/32164754 Muhoro Arthur M. et al. Insecticidal and Antiprotozoal Properties of Lichen Secondary Metabolites on Insect Vectors and Their Transmitted Protozoal Diseases to Humans. (2021) DIVERSITY (BASEL) 1424-2818 13 8<div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" mtid="10082352"><a href="/gui2/?type=authors&mode=browse&sel=10082352" target="_blank">Muhoro Arthur M. (<span class="authorship-author-name">Muhoro Arthur</span> <span class="authorAux-mtmt"> BIOLOGICAL ACTIVITIES OF LICHEN SECONDARY METAB...</span>) </a> </span> ;    <span class="author-name" mtid="10003979"><a href="/gui2/?type=authors&mode=browse&sel=10003979" target="_blank">Farkas Edit (<span class="authorship-author-name">Farkas Edit</span> <span class="authorAux-mtmt"> Növénytan, lichenológia</span>) </a> </span> <span class="author-affil"><span title="Centre for Ecological Research">CER</span>/Institute of Ecology and Botany</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;32164754" target="_blank">Insecticidal and Antiprotozoal Properties of Lichen Secondary Metabolites on Insect Vectors and Their Transmitted Protozoal Diseases to Humans</a></div> <div> <span class="journal-title">DIVERSITY (BASEL)</span> <span class="journal-issn">( <a target="_blank" href="https://portal.issn.org/resource/ISSN/1424-2818">1424-2818</a>)</span>: <span class="journal-volume">13</span> <span class="journal-issue">8</span> <span class="page"> Paper 342. </span> <span class="year">(2021)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Language: English | </span> <span class="identifiers"> <span class="id identifier oa_GOLD" title=" Gold "> <a style="color:blue" title="10.3390/d13080342" target="_blank" href="https://doi.org/10.3390/d13080342"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="000688960000001" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/000688960000001"> WoS </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="85112170678" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85112170678"> Scopus </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Journal subject: Scopus - Agricultural and Biological Sciences (miscellaneous)   Rank: Q1</div> <div class="journal-subject">Journal subject: Scopus - Ecological Modeling   Rank: Q2</div> <div class="journal-subject">Journal subject: Scopus - Ecology   Rank: Q2</div> <div class="journal-subject">Journal subject: Scopus - Nature and Landscape Conservation   Rank: Q2</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="" class="citingPub-count">Citing papers: 9</span> | Independent citation: 3 | Self citation: 6 | Unknown citation: 0 | Number of citations in WoS: 8 | Number of citations in Scopus: 8 | WoS/Scopus assigned: 9 | Number of citations with DOI: 9 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;32164754&sort=publishedYear,desc&sort=title"> Number of cited publications: 2 </a> </div> <div class="mtid"><span class="long-pub-mtid">Publication: 32164754</span> | <span class="status-data status-APPROVED"> Published </span> Core Citing | <span class="type-subtype">Journal Article ( Article ) </span> | <span class="pub-category">Scientific</span> | <span class="publication-sourceOfData">kézi felvitel</span> </div> <div class="lastModified">Last Modified: 2025.02.25. 23:57 Gusztáv Ladányi (MTMT API user, admin) </div> <pre class="comment" style="margin-top: 0; margin-bottom: 0;"><u>Comments</u>: Export Date: 31 August 2021 Correspondence Address: Farkas, E.É.; Institute of Ecology and Botany, Alkotmány u. 2–4, Hungary; email: farkas.edit@ecolres.hu</pre> </div></div>