The 3M Concept: Biomedical Translational Imaging from Molecules to Mouse to Man

Imaging keeps pervading biomedical sciences from the nanoscale to the bedside. Connecting the hierarchical levels of biomedicine with relevant imaging approaches, however, remains a challenge. Here we present a concept, called "3M", which can deliver a question, formulated at the bedside, across the wide-ranging hierarchical organization of the living organism, from the molecular level, through the small-animal scale, to whole-body human functional imaging. We present an example of nanoparticle development pipeline extending from atomic force microscopy to pre-clinical whole body imaging methods to highlight the essential features of the 3M concept, which integrates multi-scale resolution and quantification into a single logical process. Using the nanoscale to human clinical whole body approach, we present the successful development, characterisation and application of Prussian Blue nanoparticles for a variety of imaging modalities, extending it to isotope payload quantification and shape-biodistribution relationships. The translation of an idea from the bedside to the molecular level and back requires a set of novel combinatorial imaging methodologies interconnected into a logical pipeline. The proposed integrative molecules-to-mouse-to-man (3M) approach offers a promising, clinically oriented toolkit that lends the prospect of obtaining an ever-increasing amount of correlated information from as small a voxel of the human body as possible.