https://m2.mtmt.hu/ hun 2026-09-21 09:48 JournalArticle 32128093 VALIDATED true Laboratory of Molecular Pharmacology, Institute of Experimental Medicine, Budapest, Hungary Institute of Translational Medicine, Semmelweis University, Budapest, Hungary János Szentágothai Doctoral School, Semmelweis University, Budapest, Hungary Cited By :11 Export Date: 27 May 2024 CODEN: BJPCB Correspondence Address: Sperlágh, B.; Laboratory of Molecular Pharmacology, Hungary; email: sperlagh@koki.mta.hu Chemicals/CAS: calcitonin gene related peptide, 83652-28-2; clopidogrel, 113665-84-2, 120202-66-6, 90055-48-4, 94188-84-8, 120202-65-5, 120202-67-7, 894353-16-3, 744256-69-7; glyceryl trinitrate, 55-63-0, 80738-44-9; Nitroglycerin; Receptors, Purinergic P2Y12 Tradenames: psb 0739 Funding details: 2017‐1.2.1‐NKP‐2017‐00002 Funding details: 116654, 125174, 131629 Funding details: Horizon 2020 Framework Programme, H2020, 766124 Funding details: Magyar Tudományos Akadémia, MTA, PPD2019‐20/2019‐439 Funding text 1: This work was supported by the Hungarian Research and Development Fund (Grant Numbers 116654, 125174 and 131629), Hungarian Brain Research Program (2017-1.2.1-NKP-2017-00002 to B.S.), the European Union Horizon 2020 Framework Programme under the Marie Skłodowska Curie Grant Agreement No. 766124 and the Hungarian Academy of Sciences (Magyar Tudományos Akadémia) Premium Postdoctoral Research Program (PPD2019-20/2019-439 to A.I.). The authors thank the support of NMC Imaging Center at the Institute of Experimental Medicine (IEM) and the Cell Biology Center (Flow Cytometry Core Facility) of IEM. Funding text 2: This work was supported by the Hungarian Research and Development Fund (Grant Numbers 116654, 125174 and 131629), Hungarian Brain Research Program (2017‐1.2.1‐NKP‐2017‐00002 to B.S.), the European Union Horizon 2020 Framework Programme under the Marie Skłodowska Curie Grant Agreement No. 766124 and the Hungarian Academy of Sciences (Magyar Tudományos Akadémia) Premium Postdoctoral Research Program (PPD2019‐20/2019‐439 to A.I.). The authors thank the support of NMC Imaging Center at the Institute of Experimental Medicine (IEM) and the Cell Biology Center (Flow Cytometry Core Facility) of IEM. 0 false 2026-07-16T09:24:38.506+0000 2024-06-13T10:23:02.137+0000 2021-08-10T07:14:41.529+0000 true 10069606 Pintér Ibolya Katalin /api/admin/10069606 Admin true 2026-07-16T09:19:12.875+0000 2024-06-13T10:23:02.617+0000 true 10062062 Gere Tamásné /api/admin/10062062 Admin true 10062062 Admin 10066925 /api/admin/10066925 Nemec Évi true true Admin 10067876 /api/admin/10067876 Ladányi Gusztáv true true 2024-10-17T16:20:19.023+0000 true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Gölöncsér, Flóra Involvement of P2Y 12 receptors in a nitroglycerin‐induced model of migraine in male mice true true /api/journal/825 REVIEWED true false 825 false 825 true 0007-1188 1476-5381 Journal FOREIGN 178 23 4626 4645 4626 20 2021 Background and purpose: P2Y12 receptors (P2Y12 Rs) are known to regulate different forms of pain and inflammation. In this study we investigated the participation of P2Y12 Rs in an animal model of migraine. Experimental approach: We tested the effect of the centrally administered selective P2Y12 R antagonist PSB-0739, and P2Y12 R gene deficiency in acute nitroglycerin (NTG)-treated mice. Additionally, platelet depletion was used to investigate the role of platelet P2Y12 Rs during migraine-like pain. Key results: NTG induced sensory hypersensitivity of C57BL/6 wild-type (P2ry12+/+ ) mice, accompanied by an increase in c-fos and CGRP expression in the upper cervical spinal cord (C1-C2) and trigeminal nucleus caudalis (TNC). Similar changes were also observed in P2Y12 R gene-deficient (P2ry12-/- ) mice. Prophylactic intrathecal application of PSB-0739 reversed thermal hyperalgesia and head grooming time in wild-type mice but had no effect in P2ry12-/- mice; furthermore, it was also effective when applied as a post-treatment. PSB-0739 administration suppressed the expression of c-fos in C1-C2 and TNC, and decrease C1-C2 levels of dopamine and serotonin in wild-type mice. NTG treatment itself did not change adenosine diphosphate (ADP)-induced platelet activation measured by CD62P upregulation in wild-type mice. Platelet depletion by anti-mouse CD41 antibody and clopidogrel attenuated NTG-induced thermal hypersensitivity and head grooming time in mice. Conclusion and implications: Taken together, our findings show that acute inhibition of P2Y12 Rs alleviates migraine-like pain in mice, by modulating the expression of c-fos, and platelet P2Y12 Rs might contribute to this effect. Hence, it is suggested that the blockade of P2Y12 Rs may have therapeutic potential against migraine. 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<img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" mtid="10052098"><a href="/gui2/?type=authors&mode=browse&sel=10052098" target="_blank">Gölöncsér Flóra (<span class="authorship-author-name">Gölöncsér Flóra</span> <span class="authorAux-mtmt"> idegtudományok</span>) </a> </span> <span class="author-affil"><span title="Kísérleti Orvostudományi Kutatóintézet">KOKI</span>/<span title="Gyógyszerkutatási Osztály">GYO</span>/Molekuláris Farmakológia Kutatócsoport</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="91"><a href="/gui2/?type=authors&mode=browse&sel=91" target="_blank">Baranyi Mária (<span class="authorship-author-name">Baranyi Mária</span> <span class="authorAux-mtmt"> Molekuláris farmakológia</span>) </a> </span> <span class="author-affil"><span title="Kísérleti Orvostudományi Kutatóintézet">KOKI</span>/<span title="Gyógyszerkutatási 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;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10055678"><a href="/gui2/?type=authors&mode=browse&sel=10055678" target="_blank">Otrokocsi Lilla (<span class="authorship-author-name">Bereczkiné Otrokocsi Lilla</span> <span class="authorAux-mtmt"> élettudomány</span>) </a> </span> <span class="author-affil"><span title="Kísérleti Orvostudományi Kutatóintézet">KOKI</span>/<span title="Gyógyszerkutatási Osztály">GYO</span>/Molekuláris Farmakológia Kutatócsoport</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10009921"><a href="/gui2/?type=authors&mode=browse&sel=10009921" target="_blank">Benyó Zoltán (<span class="authorship-author-name">Benyó Zoltán</span> <span class="authorAux-mtmt"> Élettan és kórélettan</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Transzlációs Medicina Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10008942"><a href="/gui2/?type=authors&mode=browse&sel=10008942" target="_blank">Sperlágh Beáta ✉ (<span class="authorship-author-name">Sperlágh Beáta</span> <span class="authorAux-mtmt"> Neurofarmakológia</span>) </a> </span> <span class="author-affil"><span title="Kísérleti Orvostudományi Kutatóintézet">KOKI</span>/<span title="Gyógyszerkutatási Osztály">GYO</span>/Molekuláris Farmakológia Kutatócsoport; <span title="Semmelweis Egyetem">SE</span>/Doktori Iskola</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;32128093" target="_blank">Involvement of P2Y 12 receptors in a nitroglycerin‐induced model of migraine in male mice</a></div> <div> <span class="journal-title">BRITISH JOURNAL OF PHARMACOLOGY</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/0007-1188">0007-1188</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1476-5381">1476-5381</a>)</span>: <span 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Fund(131629), Hungarian Academy of Sciences Premium Postdoctoral Research Program(PPD2019-20/2019-439;), European Union's Horizon 2020 Research and Innovation Programme under the Marie Sklodowska Curie ...(766124), (K-125174) Támogató: NKFIH </div> <div class="lastModified">Utolsó módosítás: 2024.06.13. 12:23 Gere Tamásné (SE_KK_Admin5_GT, admin) </div> <div class="lockedBy">Központi kezelésű 2024.10.17. 18:20 Nemec Évi (MTMT Központi 3, admin) Ladányi Gusztáv (MTMT API user, admin) </div> <pre class="comment" style="margin-top: 0; margin-bottom: 0;"><u>Megjegyzés</u>: Laboratory of Molecular Pharmacology, Institute of Experimental Medicine, Budapest, Hungary Institute of Translational Medicine, Semmelweis University, Budapest, Hungary János Szentágothai Doctoral School, Semmelweis University, Budapest, Hungary Cited By :11 Export Date: 27 May 2024 ...</pre> </div></div>