Unveiling metabolic changes in marsupialized odontogenic keratocyst: A pilot study

Leite-Lima, Flavia; Bastos, Victor Coutinho; Vitorio, Jessica Gardone; Duarte-Andrade, Filipe Fideles; Pereira, Thais dos Santos Fontes; Martins-Chaves, Roberta Rayra; Cruz, Aline Fernanda; de, Lacerda Julio Cesar Tanos; Lebron, Yuri Abner Rocha; Moreira, Victor Rezende; Santos, Lucilaine Valeria de Souza; Lange, Lisete Celina; de, Macedo Adriana Nori; Diniz, Marina Goncalves; Gomes, Carolina Cavalieri; de, Castro Wagner Henriques; Canuto, Gisele Andre Baptista; Gomez, Ricardo Santiago ✉

Angol nyelvű Tudományos Szakcikk (Folyóiratcikk)
Megjelent: ORAL DISEASES 1354-523X 1601-0825 , 11 p. 2021
  • SJR Scopus - Dentistry (miscellaneous): Q1
Objective We aimed to assess which metabolic pathways would be implicated in the phenotypic changes of the epithelial lining of odontogenic keratocyst after marsupialization, comparing pre- and post-marsupialized lesions with adjacent oral mucosa. Materials and Methods Eighteen formalin-fixed and paraffin-embedded tissues from six subjects were divided into three paired groups: odontogenic keratocyst pre- (n = 6) and post-marsupialization (n = 6), and adjacent oral mucosa (n = 6). The metabolic pathways found in these groups were obtained by high-performance liquid chromatography-mass spectrometry-based untargeted metabolomics performed. Results Through putative metabolite annotation followed by pathway enrichment and predictive analysis with automated algorithms (Mummichog and Gene Set Enrichment Analysis), we found differences in many cellular processes that may be involved in inflammation, oxidative stress response, keratinocyte-basal membrane attachment, differentiation, and proliferation functions, all relevant to odontogenic keratocyst pathobiology and the phenotype acquired after marsupialization. Conclusion Our study was able to identify several metabolic pathways potentially involved in the metaplastic changes induced by marsupialization of odontogenic keratocysts. An improved comprehension of this process could pave the way for the development of targeted therapies.
Hivatkozás stílusok: IEEEACMAPAChicagoHarvardCSLMásolásNyomtatás
2021-12-08 05:25