In our current work we studied the expression of the Cowpea chlorotic mottle virus
(CCMV) capsid protein in E. coli host cell. The CCMV proteins DNA was cloned into
a vector containing a His-tag and a ubiquitin fusion partner. The gene was transformed
in E. coli BL21(DE3) Rosetta cells and expressed. The expression process was optimized
and shake flask expression was conducted on 37 degrees C and 0.1 mM IPTG concentration.
Cloning and expressing of CCMV capsid protein was successful, confirmed by sequencing
results. The protein was purified with Ni-affinity chromatography.
