https://m2.mtmt.hu/ hun 2026-04-15 11:19 JournalArticle 32039151 VALIDATED true Funding Agency and Grant Number: National Institutes of Health (NIH) [NIAID AI-050484, NHLBI HL87688, NINDS NS-29331]; Ruth L. Kirschstein National Research Service Awards Funding text: This work was supported by the National Institutes of Health (NIH) grants NIAID AI-050484, NHLBI HL87688, and NINDS NS-29331. H. M. was supported by Ruth L. Kirschstein National Research Service Awards postdoctoral fellowship. We thank Houping Ni for technical assistance. K. K. and D. W. have formed a small biotech company that receives funding from the NIH to explore the use of nucleoside-modified mRNA for gene therapy. 0 false 2026-04-02T06:58:34.928+0000 2023-10-04T14:34:04.667+0000 2021-05-28T05:35:35.443+0000 true 10030409 Karikó Katalin /api/author/10030409 Author true 10030409 2024-02-20T09:30:40.088+0000 2021-06-01T08:33:27.607+0000 true 569 Balázs András /api/admin/569 Admin true Admin 10058227 /api/admin/10058227 Áts József true 10058227 true 2023-11-06T07:03:50.271+0000 true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Kariko, Katalin Incorporation of Pseudouridine Into mRNA Yields Superior Nonimmunogenic Vector With Increased Translational Capacity and Biological Stability true true /api/journal/30657 REVIEWED true false 30657 false 30657 true 1525-0016 1525-0024 Journal FOREIGN 16 11 1833 1840 1833 8 2008 In vitro-transcribed mRNAs encoding physiologically important proteins have considerable potential for therapeutic applications. However, in its present form, mRNA is unfeasible for clinical use because of its labile and immunogenic nature. Here, we investigated whether incorporation of naturally modified nucleotides into transcripts would confer enhanced biological properties to mRNA. We found that mRNAs containing pseudouridines have a higher translational capacity than unmodified mRNAs when tested in mammalian cells and lysates or administered intravenously into mice at 0.015-0.15 mg/kg doses. The delivered mRNA and the encoded protein could be detected in the spleen at 1, 4, and 24 hours after the injection, where both products were at significantly higher levels when pseudouridine-containing mRNA was administered. Even at higher doses, only the unmodified mRNA was immunogenic, inducing high serum levels of interferon-alpha ( IFN-alpha). These findings indicate that nucleoside modification is an effective approach to enhance stability and translational capacity of mRNA while diminishing its immunogenicity in vivo. Improved properties conferred by pseudouridine make such mRNA a promising tool for both gene replacement and vaccination. 2021 true true true false false NONE 2026-04-02 false 1439 7 1439 1374 13 1425 1424 1382 1095 1439 1439 1374 1374 1374 65 0 1439 1374 1439 1374 0 3 3 Folyóiratcikk Journal Article 24 1384 Könyvrészlet Chapter in Book 25 52 Könyv Book 23 3 Egyéb konferenciaközlemény Conference paper 31 0 Egyéb konferenciakötet Conference proceedings 32 0 Oltalmi formák Protection forms 26 0 Disszertáció Thesis 28 0 Egyéb Miscellaneous 29 0 Alkotás Achievement 22 0 Kutatási adat Research data 33 0 Folyóiratcikk Journal Article 24 0 0 0 Könyvrészlet Chapter in Book 25 0 0 0 Könyv Book 23 0 0 0 Egyéb konferenciaközlemény Conference paper 31 0 0 0 Egyéb konferenciakötet Conference proceedings 32 0 0 0 Oltalmi formák Protection forms 26 0 0 0 Disszertáció Thesis 28 0 0 0 Egyéb Miscellaneous 29 0 0 0 Alkotás Achievement 22 0 0 0 Kutatási adat Research data 33 0 0 0 2010 0 4 3 4 3 2011 0 4 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autype0"> <span class="author-name" mtid="10030409"><a href="/gui2/?type=authors&mode=browse&sel=10030409" target="_blank">Kariko Katalin ✉ (<span class="authorship-author-name">Karikó Katalin</span> <span class="authorAux-mtmt"> biofizika</span>) </a> </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Muramatsu Hiromi </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Welsh Frank A. </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Ludwig Janos </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Kato Hiroki </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Akira Shizuo </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Weissman Drew </span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;32039151" target="_blank">Incorporation of Pseudouridine Into mRNA Yields Superior Nonimmunogenic Vector With Increased Translational Capacity and Biological Stability</a></div> <div> <span class="journal-title">MOLECULAR THERAPY</span> <span 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class="pub-category">Tudományos</span> | <span class="publication-sourceOfData">WOS TAGGED</span> </div> <div class="lastModified">Utolsó módosítás: 2023.10.04. 16:34 Bernátskyné Babus Csilla (SZTE admin4) </div> <div class="lockedBy">Központi kezelésű 2023.11.06. 08:03 Áts József (ÁtsJ adm2-3, admin) </div> <pre class="comment" style="margin-top: 0; margin-bottom: 0;"><u>Megjegyzés</u>: Funding Agency and Grant Number: National Institutes of Health (NIH) [NIAID AI-050484, NHLBI HL87688, NINDS NS-29331]; Ruth L. Kirschstein National Research Service Awards Funding text: This work was supported by the National Institutes of Health (NIH) grants NIAID AI-050484, NHLBI HL87688, and NINDS NS-29331. H. M. was supported by Ruth L. Kirschstein National Research Service Awar...</pre> </div></div>