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Biological Research Centre, Eotvos Loránd Research Network, Institute of Genetics, Szeged, H-6726, Hungary
Doctoral School of Biology, University of Szeged, Szeged, H-6720, Hungary
Export Date: 27 May 2021
Biological Research Centre, Institute of Genetics, Szeged, H-6726, Hungary
Doctoral School of Biology, University of Szeged, Szeged, H-6720, Hungary
Export Date: 31 August 2021
Correspondence Address: Unk, I.; Biological Research Centre, Temesvari krt. 62, Hungary; email: unk.ildiko@brc.hu
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The Zn-finger of Saccharomyces cerevisiae Rad18 and its adjacent region mediate interaction with Rad5
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DNA damages that hinder the movement of the replication complex can ultimately lead to cell death. To avoid that, cells possess several DNA damage bypass mechanisms. The Rad18 ubiquitin ligase controls error-free and mutagenic pathways that help the replication complex to bypass DNA lesions by monoubiquitylating PCNA at stalled replication forks. In Saccharomyces cerevisiae, two of the Rad18 governed pathways are activated by monoubiquitylated PCNA and they involve translesion synthesis polymerases, whereas a third pathway needs subsequent polyubiquitylation of the same PCNA residue by another ubiquitin ligase the Rad5 protein, and it employs template switching. The goal of this study was to dissect the regulatory role of the multidomain Rad18 in DNA damage bypass using a structure-function based approach. Investigating deletion and point mutant RAD18 variants in yeast genetic and yeast two-hybrid assays we show that the Zn-finger of Rad18 mediates its interaction with Rad5, and the N-terminal adjacent region is also necessary for Rad5 binding. Moreover, results of the yeast two-hybrid and in vivo ubiquitylation experiments raise the possibility that direct interaction between Rad18 and Rad5 might not be necessary for the function of the Rad5 dependent pathway. The presented data also reveal that yeast Rad18 uses different domains to mediate its association with itself and with Rad5. Our results contribute to better understanding of the complex machinery of DNA damage bypass pathways. © The Author(s) 2021. Published by Oxford University Press on behalf of Genetics Society of America.
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<div class="JournalArticle Publication short-list"> <div class="authors"> <span class="author-name" mtid="10055123"> <a href="/gui2/?type=authors&mode=browse&sel=10055123" target="_blank">Frittmann, O.</a> </span> <span class="author-type"> </span> ; <span class="author-name" mtid="10043537"> <a href="/gui2/?type=authors&mode=browse&sel=10043537" target="_blank">Gali, V.K.</a> </span> <span class="author-type"> </span> ; <span class="author-name" mtid="10043539"> <a href="/gui2/?type=authors&mode=browse&sel=10043539" target="_blank">Halmai, M.</a> </span> <span class="author-type"> </span> ; <span class="author-name" mtid="10081960"> <a href="/gui2/?type=authors&mode=browse&sel=10081960" target="_blank">Toth, R.</a> </span> <span class="author-type"> </span> ; <span class="author-name" mtid="10010557"> <a href="/gui2/?type=authors&mode=browse&sel=10010557" target="_blank">Gyorfy, Z.</a> </span> <span class="author-type"> </span> ; <span class="author-name" mtid="10001859"> <a href="/gui2/?type=authors&mode=browse&sel=10001859" target="_blank">Balint, E.</a> </span> <span class="author-type"> </span> ; <span class="author-name" mtid="10011517"> <a href="/gui2/?type=authors&mode=browse&sel=10011517" target="_blank">Unk, I. ✉</a> </span> <span class="author-type"> </span> </div ><div class="title"><a href="/gui2/?mode=browse¶ms=publication;32037907" mtid="32037907" target="_blank">The Zn-finger of Saccharomyces cerevisiae Rad18 and its adjacent region mediate interaction with Rad5</a></div> <div class="pub-info"> <span class="journal-title">G3-GENES GENOMES GENETICS</span> <span class="journal-volume">11</span> : <span class="journal-issue">4</span> <span class="page"> Paper: jkab041 , 12 p. </span> <span class="year">(2021)</span> </div> <div class="pub-end"><div class="identifier-list"> <span class="identifiers"> <span class="id identifier oa_GOLD" title=" Gold "> <a style="color:blue" title="10.1093/g3journal/jkab041" target="_blank" href="https://doi.org/10.1093/g3journal/jkab041"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="000651852100007" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/000651852100007"> WoS </a> </span> <span class="id identifier oa_GOLD" title=" Gold "> <a style="color:black" title="137237" target="_blank" href="http://real.mtak.hu/137237"> REAL </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="85104899735" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85104899735"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="33570581" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=33570581&dopt=Abstract"> PubMed </a> </span> </span> </div> <div class="short-pub-prop-list"> <span class="short-pub-mtid"> Közlemény:32037907 </span> <span class="status-holder"><span class="status-data status-VALIDATED"> Egyeztetett </span></span> <span class="pub-core">Forrás Idéző </span> <span class="pub-type">Folyóiratcikk (Szakcikk ) </span> <!-- && !record.category.scientific --> <span class="pub-category">Tudományos</span> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="Nyilvános idézőközlemények összesen, említések nélkül" class="citingPub-count">Nyilvános idéző összesen: 3</span> | Független: 1 | Függő: 2 | Nem jelölt: 0 | WoS jelölt: 3 | Scopus jelölt: 3 | WoS/Scopus jelölt: 3 | DOI jelölt: 3 </div> </div> </div> </div><div class="JournalArticle Publication long-list">
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<div class="title"><a href="/gui2/?mode=browse¶ms=publication;32037907" target="_blank">The Zn-finger of Saccharomyces cerevisiae Rad18 and its adjacent region mediate interaction with Rad5</a></div> <div> <span class="journal-title">G3-GENES GENOMES GENETICS</span>
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<div class="lastModified">Utolsó módosítás: 2022.01.31. 09:15 Patkósné Hanesz Andrea (SZBK-4-es admin)
</div>
<pre class="comment" style="margin-top: 0; margin-bottom: 0;"><u>Megjegyzés</u>: Biological Research Centre, Eotvos Loránd Research Network, Institute of Genetics, Szeged, H-6726, Hungary
Doctoral School of Biology, University of Szeged, Szeged, H-6720, Hungary
Export Date: 27 May 2021
Biological Research Centre, Institute of Genetics, Szeged, H-6726, Hungary
Doctoral School of Biology, University of Sz...</pre>
</div></div>