TÉMATERÜLETI KIVÁLÓSÁGI PROGRAM 2020(2020-4.1.1-TKP2020)
(TKP2020-IKA-08) Támogató: NKFIA
(739593) Támogató: Horizon 2020
(128322) Támogató: Nemzeti Kutatás, Fejlesztés és Innovációs Iroda
Szakterületek:
Molekuláris biofizika
Molekuláris kémia
Bc-DLFL.1 is a novel spontaneous, high-grade transplantable mouse B-cell lymphoma
model for selective serosal propagation. These cells attach to the omentum and mesentery
and show dissemination in mesenteric lymph nodes. We aimed to investigate its early
stage spread at one day post-intraperitoneal inoculation of lymphoma cells (n = 18
mice), and its advanced stage at seven days post-inoculation with in vivo [18F]FDG-PET
and [18F]PET/MRI, and ex vivo by autoradiography and Cherenkov luminescence imaging
(CLI). Of the early stage group, nine animals received intraperitoneal injections,
and nine received intravenous [18F]FDG injections. The advanced stage group (n = 3)
received intravenous FDG injections. In the early stage, using autoradiography we
observed a marked accumulation in the mesentery after intraperitoneal FDG injection.
Using other imaging methods and autoradiography, following the intravenous injection
of FDG no accumulations were detected. At the advanced stage, tracer accumulation
was clearly detected in mesenteric lymph nodes and in the peritoneum after intravenous
administration using PET. We confirmed the results with immunohistochemistry. Our
results in this model highlight the importance of local FDG administration during
diagnostic imaging to precisely assess early peritoneal manifestations of other malignancies
(colon, stomach, ovary). These findings also support the importance of applying topical
therapies, in addition to systemic treatments in peritoneal cancer spread.
