https://m2.mtmt.hu/ hun 2026-10-24 08:53 JournalArticle 31937727 VALIDATED true Department of Cardiac Surgery, Laboratory of Cardiac Surgery, University Hospital Heidelberg, INF 326, Heidelberg, 69120, Germany Department of Cardiac Surgery, University Hospital Halle (Saale), Halle, 06120, Germany Heart and Vascular Center, Semmelweis University, Budapest, 1122, Hungary Cited By :2 Export Date: 9 October 2021 Correspondence Address: Korkmaz-Icöz, S.; Department of Cardiac Surgery, INF 326, Germany; email: korkmaz@uni-heidelberg.de Chemicals/CAS: 3 nitrotyrosine, 3604-79-3; acetylcholine, 51-84-3, 60-31-1, 66-23-9; caspase 12, 230951-53-8; caspase 3, 169592-56-7; caspase 8; caspase 9, 180189-96-2; intercellular adhesion molecule 1, 126547-89-5; myeloperoxidase; sodium chloride, 7647-14-5, 23724-87-0, 49658-21-1; Culture Media, Conditioned 0 false 2026-10-21T05:29:15.930+0000 2022-08-29T07:48:05.945+0000 2021-03-30T08:51:04.830+0000 true 10062250 Molnár-Taga Márta /api/admin/10062250 Admin true 10062250 2025-09-24T08:06:54.522+0000 2021-03-30T08:53:42.315+0000 true 10062250 Molnár-Taga Márta /api/admin/10062250 Admin true 10062250 true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Korkmaz-Icoez, Sevil Mesenchymal stem cell-derived conditioned medium protects vascular grafts of brain-dead rats against in vitro ischemia/reperfusion injury true true /api/journal/10014334 REVIEWED true false 10014334 false 10014334 true 1757-6512 1757-6512 Journal FOREIGN 12 1 144 144 13 2021 Background Brain death (BD) has been suggested to induce coronary endothelial dysfunction. Ischemia/reperfusion (IR) injury during heart transplantation may lead to further damage of the endothelium. Previous studies have shown protective effects of conditioned medium (CM) from bone marrow-derived mesenchymal stem cells (MSCs) against IR injury. We hypothesized that physiological saline-supplemented CM protects BD rats' vascular grafts from IR injury. Methods The CM from rat MSCs, used for conservation purposes, indicates the presence of 23 factors involved in apoptosis, inflammation, and oxidative stress. BD was induced by an intracranial-balloon. Controls were subjected to a sham operation. After 5.5 h, arterial pressures were measured in vivo. Aortic rings from BD rats were harvested and immediately mounted in organ bath chambers (BD group, n = 7) or preserved for 24 h in 4 degrees C saline-supplemented either with a vehicle (BD-IR group, n = 8) or CM (BD-IR+CM group, n = 8), prior to mounting. Vascular function was measured in vitro. Furthermore, immunohistochemistry and quantitative real-time polymerase chain reaction (qRT-PCR) have been performed. Results BD in donors was associated with significantly impaired hemodynamic parameters and higher immunoreactivity of aortic myeloperoxidase (MPO), nitrotyrosine, caspase-3, caspase-8, caspase-9, and caspase-12 compared to sham-operated rats. In organ bath experiments, impaired endothelium-dependent vasorelaxation to acetylcholine in the BD-IR group compared to BD rats was significantly improved by CM (maximum relaxation to acetylcholine: BD 81 +/- 2% vs. BD-IR 50 +/- 3% vs. BD-IR + CM 72 +/- 2%, p < 0.05). Additionally, the preservation of BD-IR aortic rings with CM significantly lowered MPO, caspase-3, caspase-8, and caspase-9 immunoreactivity compared with the BD-IR group. Furthermore, increased mRNA expression of vascular cell adhesion molecule (VCAM)-1 and intercellular adhesion molecule (ICAM)-1 in the aortas from the BD-IR rats compared to BD group were significantly decreased by CM. Conclusions The preservation of BD rats' vascular grafts with CM alleviates endothelial dysfunction following IR injury, in part, by reducing levels of inflammatory response and caspase-mediated apoptosis. 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false true Reference 19425978 /api/reference/19425978 42 false true Reference 19425977 /api/reference/19425977 43 false true Reference 19425976 /api/reference/19425976 44 false true /api/publication/31937727 <div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" >Korkmaz-Icoez Sevil ✉ </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Zhou Pengyu </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Guo Yuxing </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Loganathan Sivakkanan </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Brlecic Paige </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10013969"><a href="/gui2/?type=authors&mode=browse&sel=10013969" target="_blank">Radovits Tamas (<span class="authorship-author-name">Radovits Tamás</span> <span class="authorAux-mtmt"> kardiológia, érgyógyászat</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Klinikum">K</span>/<span title="Városmajori Szív- és Érgyógyászati Klinika">VAROSMAJOR_SZÍVÉRGYÓGY</span>/Kardiológia Központ - Kardiológiai Tanszék</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10069723"><a href="/gui2/?type=authors&mode=browse&sel=10069723" target="_blank">Sayour Alex Ali (<span class="authorship-author-name">Sayour Alex Ali</span> <span class="authorAux-mtmt"> kardiológia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Klinikum">K</span>/<span title="Városmajori Szív- és Érgyógyászati Klinika">VAROSMAJOR_SZÍVÉRGYÓGY</span>/Kardiológia Központ - 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</div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;31937727" target="_blank">Mesenchymal stem cell-derived conditioned medium protects vascular grafts of brain-dead rats against in vitro ischemia/reperfusion injury</a></div> <div> <span class="journal-title">STEM CELL RESEARCH & THERAPY</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/1757-6512">1757-6512</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1757-6512">1757-6512</a>)</span>: <span class="journal-volume">12</span> <span class="journal-issue">1</span> <span class="page"> Paper 144. 13 p. </span> <span class="year">(2021)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Nyelv: Angol | </span> <span class="identifiers"> <span class="id identifier oa_GOLD" title=" Gold "> <a style="color:blue" title="10.1186/s13287-021-02166-3" target="_blank" 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Scopus - Medicine (miscellaneous)&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Molecular Medicine&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Cell Biology&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q2</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="Nyilvános idézőközlemények összesen, említések nélkül" class="citingPub-count">Nyilvános idéző összesen: 18</span> | Független: 13 | Függő: 5 | Nem jelölt: 0 | WoS jelölt: 17 | Scopus jelölt:&nbsp;16 | WoS/Scopus jelölt:&nbsp;17 | DOI jelölt:&nbsp;18 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;31937727&sort=publishedYear,desc&sort=title"> Idézett közlemények száma: 9 </a> </div> <div class="mtid"><span class="long-pub-mtid">Közlemény: 31937727</span> | <span class="status-data status-VALIDATED"> Egyeztetett </span> Forrás Idéző | <span class="type-subtype">Folyóiratcikk ( Szakcikk ) </span> | <span class="pub-category">Tudományos</span> | <span class="publication-sourceOfData">WOS TAGGED</span> </div> <div class="funder"> National Heart Program(NVKP-16-1-2016-0017) Támogató: NKFIH, Thematic Excellence Program (Semmelweis University)(2020-4.1.1.-TKP2020) Támogató: Innovációs és Technológiai Minisztérium, (Open Access funding by Projekt DEAL) </div> <div class="lastModified">Utolsó módosítás: 2022.08.29. 09:48 Hegedűs Anikó (SE_AOK_Szívseb_Admin5_HA, admin) </div> <pre class="comment" style="margin-top: 0; margin-bottom: 0;"><u>Megjegyzés</u>: Department of Cardiac Surgery, Laboratory of Cardiac Surgery, University Hospital Heidelberg, INF 326, Heidelberg, 69120, Germany Department of Cardiac Surgery, University Hospital Halle (Saale), Halle, 06120, Germany Heart and Vascular Center, Semmelweis University, Budapest, 1122, Hungary Cited By :2 ...</pre> </div></div>