https://m2.mtmt.hu/ hun 2026-09-22 21:31 JournalArticle 31884048 VALIDATED true Export Date: 20 February 2026; Cited By: 14; Correspondence Address: K. Kolev; Department of Biochemistry, Institute of Biochemistry and Molecular Biology, Semmelweis University, Budapest, Hungary; email: Krasimir.Kolev@eok.sote.hu; CODEN: JTHOA; 0 false 2026-08-12T08:45:23.960+0000 2026-03-10T14:24:59.735+0000 2021-02-21T09:09:02.819+0000 true 10000059 Kolev Kraszimir Nikolaev /api/author/10000059 Author true 10000059 2026-06-23T11:52:04.190+0000 2021-10-14T18:56:36.900+0000 true 10067876 Ladányi Gusztáv /api/admin/10067876 Admin true true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Komorowicz, Erzsébet Size- and charge-dependent modulation of the lytic susceptibility and mechanical stability of fibrin-histone clots by heparin and polyphosphate variants true true /api/journal/10001276 REVIEWED true false 10001276 false 10001276 true 1538-7933 1538-7836 Journal FOREIGN 19 1307 1318 1307 12 2021 Neutrophil extracellular traps (NETs) containing DNA and histones are expelled from neutrophils in infection and thrombosis. Heparins, anticoagulant polyanions, can neutralize histones with a potential therapeutic advantage in sepsis. Polyphosphates, procoagulant polyanions, are released by platelets and microorganisms.To characterize the combined effects of NET components and polyanions on clot structure, mechanical properties and lytic susceptibility.Scanning electron microscopy, pressure-driven permeation, turbidimetry, and oscillation rheometry were used for the characterization of the structure, viscoelasticity, and kinetics of formation and lysis of fibrin and plasma clots containing histones+/-DNA in combination with unfractionated heparin, its desulfated derivatives, low molecular weight heparin (LMWH), pentasaccharide, and polyphosphates of different sizes.Histones and DNA inhibited fibrin lysis by plasmin, but this behavior was not neutralized by negatively charged heparins or short polyphosphates. Rather, fibrin lysis was further inhibited by added polyanions. Histones inhibited plasma clot lysis by tissue plasminogen activator and the response to added heparin was size dependent. Unfractionated heparin, LMWH, and pentasaccharide had no effect, exacerbated, or reversed histone inhibition, respectively. Histones increased the mechanical strength of fibrin, which was exacerbated by smaller heparin and polyphosphate molecules. Histones increased fibrin diameter and pore size of fibrin clots and this effect was neutralized by all heparin variants but enhanced by polyphosphates.Despite their common polyanionic character, heparins and polyphosphates exert distinct effects on fibrin mechanical and fibrinolytic stability. Anti-fibrinolytic effects of histones were more often enhanced by polyanions not counteracted. Careful selection of anti-histone strategies is required if they are to be combined with thrombolytic therapy. 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2 2026 0 3 3 3 3 D1 false false false false 2021-05-30T10:12:57.560+0000 Anyag- és Környezetkémiai Intézet (TTK); Biofizikai és Sugárbiológiai Intézet (SE / AOK / I); Biokémiai Tanszék (SE / AOK / I / BMBI); Biológiai Nanokémiai Kutatócsoport (HRN TTK / AKI); Semmelweis Egyetem 6 23 5 8 0 true Language 10002 /api/language/10002 Angol English true 2 true PersonAuthorship 95553571 /api/authorship/95553571 1 0.125 true false false Author 10010953 /api/author/10010953 Komorowicz Erzsébet true 10010953 true Komorowicz Erzsébet true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 95553572 /api/authorship/95553572 2 0.125 false false false Balázs Nóra true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 95553573 /api/authorship/95553573 3 0.125 false false false Author 10021854 /api/author/10021854 Tanka-Salamon Anna true 10021854 true Tanka-Salamon Anna true false false AuthorshipType 1 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/api/keyword/1020329 true 1020329 true SjrRating 11229847 /api/sjrrating/11229847 7 0.1 journal RatingType 10002 /api/ratingtype/10002 sjr true true ClassificationExternal 2720 /api/classificationexternal/2720 true 2720 true D1 DIRECT true true /api/publication/31884048 <div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" mtid="10010953"><a href="/gui2/?type=authors&mode=browse&sel=10010953" target="_blank">Komorowicz Erzsébet (<span class="authorship-author-name">Komorowicz Erzsébet</span> <span class="authorAux-mtmt"> Orvosi biokémia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/<span title="Biokémiai és Molekuláris Biológiai Intézet">BMBI</span>/Biokémiai Tanszék</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Balázs Nóra </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10021854"><a href="/gui2/?type=authors&mode=browse&sel=10021854" target="_blank">Tanka-Salamon Anna (<span class="authorship-author-name">Tanka-Salamon Anna</span> <span class="authorAux-mtmt"> Biokémia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/<span title="Biokémiai és Molekuláris Biológiai Intézet">BMBI</span>/Biokémiai Tanszék</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10014807"><a href="/gui2/?type=authors&mode=browse&sel=10014807" target="_blank">Varga Zoltán (<span class="authorship-author-name">Varga Zoltán</span> <span class="authorAux-mtmt"> biofizika, kolloidkémia</span>) </a> </span> <span class="author-affil">Semmelweis Egyetem; <span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Biofizikai és Sugárbiológiai Intézet; <span title="Természettudományi Kutatóközpont">TTK</span>/Anyag- és Környezetkémiai Intézet; <span title="Természettudományi Kutatóközpont">TTK</span>/<span title="Anyag- és Környezetkémiai Intézet">AKI</span>/Biológiai Nanokémiai Kutatócsoport</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10014812"><a href="/gui2/?type=authors&mode=browse&sel=10014812" target="_blank">Szabó László (<span class="authorship-author-name">Szabó László</span> <span class="authorAux-mtmt"> Biológia, Elektronmikroszkópia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/<span title="Biokémiai és Molekuláris Biológiai Intézet">BMBI</span>/Biokémiai Tanszék</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10014155"><a href="/gui2/?type=authors&mode=browse&sel=10014155" target="_blank">Bóta Attila (<span class="authorship-author-name">Bóta Attila</span> <span class="authorAux-mtmt"> Biofizikai kémia, Kolloidika</span>) </a> </span> <span class="author-affil"><span title="Természettudományi Kutatóközpont">TTK</span>/Anyag- és Környezetkémiai Intézet; <span title="Természettudományi Kutatóközpont">TTK</span>/<span title="Anyag- és Környezetkémiai Intézet">AKI</span>/Biológiai Nanokémiai Kutatócsoport</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Longstaff Colin </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10000059"><a href="/gui2/?type=authors&mode=browse&sel=10000059" target="_blank">Kolev Krasimir ✉ (<span class="authorship-author-name">Kolev Kraszimir Nikolaev</span> <span class="authorAux-mtmt"> Orvosi biokémia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/<span title="Biokémiai és Molekuláris Biológiai Intézet">BMBI</span>/Biokémiai Tanszék</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;31884048" target="_blank">Size- and charge-dependent modulation of the lytic susceptibility and mechanical stability of fibrin-histone clots by heparin and polyphosphate variants</a></div> <div> <span class="journal-title">JOURNAL OF THROMBOSIS AND HAEMOSTASIS</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/1538-7933">1538-7933</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1538-7836">1538-7836</a>)</span>: <span class="journal-volume">19</span> <span class="page"> pp 1307-1318 </span> <span class="year">(2021)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Nyelv: Angol | </span> <span class="identifiers"> <span 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title="https://www.scopus.com/inward/record.uri?eid=2-s2.0-85101068727&doi=10.1111%2Fjth.15258&partnerID=40&md5=bb0da97859370c84ada0fbfa43aeb807" target="_blank" href="https://www.scopus.com/inward/record.uri?eid=2-s2.0-85101068727&doi=10.1111%2Fjth.15258&partnerID=40&md5=bb0da97859370c84ada0fbfa43aeb807"> Egyéb URL </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="https://linkinghub.elsevier.com/retrieve/pii/S1538783622007437" target="_blank" href="https://linkinghub.elsevier.com/retrieve/pii/S1538783622007437"> Egyéb URL </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Folyóirat szakterülete: Scopus - Hematology&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;D1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Medicine (miscellaneous)&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;D1</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="Nyilvános idézőközlemények összesen, említések nélkül" class="citingPub-count">Nyilvános idéző összesen: 17</span> | Független: 13 | Függő: 4 | Nem jelölt: 0 | WoS jelölt: 16 | Scopus jelölt:&nbsp;15 | WoS/Scopus jelölt:&nbsp;17 | DOI jelölt:&nbsp;17 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;31884048&sort=publishedYear,desc&sort=title"> Idézett közlemények száma: 9 </a> </div> <div class="mtid"><span class="long-pub-mtid">Közlemény: 31884048</span> | <span class="status-data status-VALIDATED"> Egyeztetett </span> Forrás Idéző | <span class="type-subtype">Folyóiratcikk ( Szakcikk ) </span> | <span class="pub-category">Tudományos</span> | <span class="publication-sourceOfData">kézi felvitel</span> </div> <div class="funder"> (129528) Támogató: NKFIH, (STIA- 2020-OTKA/137209) </div> <div class="lastModified">Utolsó módosítás: 2026.03.10. 15:24 Kondor Zóra (SE_KK_Admin5_KZ, admin) </div> <pre class="comment" style="margin-top: 0; margin-bottom: 0;"><u>Megjegyzés</u>: Export Date: 20 February 2026; Cited By: 14; Correspondence Address: K. Kolev; Department of Biochemistry, Institute of Biochemistry and Molecular Biology, Semmelweis University, Budapest, Hungary; email: Krasimir.Kolev@eok.sote.hu; CODEN: JTHOA;</pre> </div></div>