Topography, Spike Dynamics, and Nanomechanics of Individual Native SARS-CoV-2 Virions

Kiss, B. [Kiss, Bálint (Biofizika), szerző] Biofizikai és Sugárbiológiai Intézet (SE / AOK / I); Kis, Z.* [Kis, Zoltán (Mikrobiológia, Vi...), szerző] Orvosi Mikrobiológiai Intézet (SE / AOK / I); Pályi, B.; Kellermayer, M.S.Z. ✉ [Kellermayer, Miklós (Biofizika), szerző] Biofizikai és Sugárbiológiai Intézet (SE / AOK / I); HCEMM-SE In Vivo Képalkotó Műszerközpont (SE / AOK / I / BSI)

Angol nyelvű Szakcikk (Folyóiratcikk) Tudományos
Megjelent: NANO LETTERS 1530-6984 1530-6992 21 (6) pp. 2675-2680 2021
  • SJR Scopus - Mechanical Engineering: D1
Azonosítók
Támogatások:
  • (K124966) Támogató: NKFIH
  • (NVKP-16-1-2016-0017) Támogató: Nemzeti Kutatási, Fejlesztési és Innovációs Hivatal
  • Nemzeti Bionika Program(ED_17-1-2017-0009)
  • (739593) Támogató: Horizon 2020
  • (Higher Education Institutional Excellence Programme of the Ministry for Innovation and Technology in Hungary, within the framework of the Therapeutic Development thematic programme of the Semmelweis University)
SARS-CoV-2, the virus responsible for the current COVID-19 pandemic, displays a corona-shaped layer of spikes which play a fundamental role in the infection process. Recent structural data suggest that the spikes possess orientational freedom and the ribonucleoproteins segregate into basketlike structures. How these structural features regulate the dynamic and mechanical behavior of the native virion are yet unknown. By imaging and mechanically manipulating individual, native SARS-CoV-2 virions with atomic force microscopy, here, we show that their surface displays a dynamic brush owing to the flexibility and rapid motion of the spikes. The virions are highly compliant and able to recover from drastic mechanical perturbations. Their global structure is remarkably temperature resistant, but the virion surface becomes progressively denuded of spikes upon thermal exposure. The dynamics and the mechanics of SARS-CoV-2 are likely to affect its stability and interactions. © 2021 American Chemical Society.
Hivatkozás stílusok: IEEEACMAPAChicagoHarvardCSLMásolásNyomtatás
2024-07-14 19:44