The mechanism of non-blocking inhibition of sodium channels revealed by conformation-selective photolabeling

Földi, Mátyás C [Földi, Mátyás Csaba (Földi Mátyás Csaba), szerző] Növényvédelmi Intézet (ATK); Pesti, Krisztina [Pesti, Krisztina (Neurofarmakológia), szerző] Biokémiai Tanszék (ELTE / TTK / Bio_I); MTA-ELTE-NAP B Opto-Neurofarmakológiai Kutatócs... (ELTE / TTK / Bio_I); Doktori Iskola (SE); Zboray, Katalin [Zboray, Katalin (Biológia), szerző] Növényvédelmi Intézet (ATK); Toth, Adam V; Hegedűs, Tamás [Hegedűs, Tamás (Biokémia, biofizi...), szerző] Biofizikai és Sugárbiológiai Intézet (SE / AOK / I); Málnási-Csizmadia, András [Málnási Csizmadia, András (Szerkezeti biokémia), szerző] Biokémiai Tanszék (ELTE / TTK / Bio_I); MTA-ELTE Motor Farmakológiai Kutatócsoport (ELTE / TTK / Bio_I); Lukacs, Peter** [Lukács, Péter (Elektrofiziológia), szerző] Növényvédelmi Intézet (ATK); MTA-ELTE-NAP B Opto-Neurofarmakológiai Kutatócs... (ELTE / TTK / Bio_I); Mike, Árpád ✉ [Mike, Árpád (Neurofarmakológia), szerző] Növényvédelmi Intézet (ATK); Biokémiai Tanszék (ELTE / TTK / Bio_I); MTA-ELTE-NAP B Opto-Neurofarmakológiai Kutatócs... (ELTE / TTK / Bio_I)

Angol nyelvű Tudományos Szakcikk (Folyóiratcikk)
Megjelent: BRITISH JOURNAL OF PHARMACOLOGY 0007-1188 1476-5381 178 (5) pp. 1200-1217 2021
  • SJR Scopus - Pharmacology: D1
Sodium channel inhibitors can be used to treat hyperexcitability-related diseases, including epilepsies, pain syndromes, neuromuscular disorders, and cardiac arrhythmias. The applicability of these drugs is limited by their nonspecific effect on physiological function. They act mainly by sodium channel block and in addition by modulation of channel kinetics. While channel block inhibits healthy and pathological tissue equally, modulation can preferentially inhibit pathological activity. An ideal drug designed to target the sodium channels of pathological tissue would act predominantly by modulation. Thus far, no such drug has been described.Patch-clamp experiments with ultra-fast solution exchange and photolabeling-coupled electrophysiology were applied to describe the unique mechanism of riluzole on Nav1.4 sodium channels. In silico docking experiments were used to study the molecular details of binding.We present evidence that riluzole acts predominantly by non-blocking modulation. We propose that, being a relatively small molecule, riluzole is able to stay bound to the binding site, but nonetheless stay off the conduction pathway, by residing in one of the fenestrations. We demonstrate how this mechanism can be recognized.Our results identify riluzole as the prototype of this new class of sodium channel inhibitors. Drugs of this class are expected to selectively prevent hyperexcitability, while having minimal effect on cells firing at a normal rate from a normal resting potential.
Hivatkozás stílusok: IEEEACMAPAChicagoHarvardCSLMásolásNyomtatás
2021-05-14 13:04