https://m2.mtmt.hu/ hun 2026-09-22 17:41 JournalArticle 31809540 VALIDATED true 0 false 2026-05-20T12:31:16.428+0000 2022-06-07T13:26:17.595+0000 2021-01-13T19:56:19.543+0000 true 10021946 Mizsei Réka /api/author/10021946 Author true 10021946 2022-12-21T19:23:02.490+0000 2022-06-07T13:26:17.604+0000 true 10015546 Berhidi Anna /api/admin/10015546 Admin true 10015546 true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Mizsei, Réka A general chemical crosslinking strategy for structural analyses of weakly interacting proteins applied to preTCR-pMHC complexes true true /api/journal/2295 REVIEWED true false 2295 false 2295 true 0021-9258 1083-351X Journal FOREIGN 296 100255 100255 16 2021 T lymphocytes discriminate between healthy versus infected or cancerous cells via T-cell receptor mediated recognition of peptides bound and presented by cell-surface-expressed major histocompatibility complex molecules (MHCs). Pre-T cell receptors (preTCRs) on thymocytes foster development of αβT lymphocytes through their β chain interaction with MHC displaying self-peptides on thymic epithelia. The specific binding of a preTCR with a peptide-MHC complex (pMHC) has been identified previously as forming a weak affinity complex with a distinct interface from that of mature αβTCR. However, a lack of appropriate tools has limited prior efforts to investigate this unique interface. Here we designed a small-scale linkage screening protocol using bismaleimide linkers for determining residue-specific distance constraints between transiently interacting protein pairs in solution. Employing linkage distance restraint-guided molecular modeling, we report the oriented solution docking geometry of a preTCRβ-pMHC interaction. The linkage model of preTCRβ-pMHC complex was independently verified with paramagnetic pseudocontact chemical shift (PCS) NMR of the unlinked protein mixtures. Using linkage screens, we show that the preTCR binds with differing affinities to peptides presented by MHC in solution. Moreover, the C-terminal peptide segment is a key determinant in preTCR-pMHC recognition. We also describe the process for future large-scale production and purification of the linked constructs for NMR, X-ray crystallography and single molecule electron microscopy studies. Funding 2014665 /api/funding/2014665 false true Funding 2000680 /api/funding/2000680 false true Funding 2014666 /api/funding/2014666 false true Funding 2014667 /api/funding/2014667 false true Funding 2014668 /api/funding/2014668 false true true true 2021 true true true false true false GOLD 2026-05-20 true https://doi.org/10.1016/j.jbc.2021.100255 4 0 4 1 0 4 4 4 0 4 4 1 1 1 3 0 4 1 4 1 0 1 1 Folyóiratcikk Journal Article 24 4 Könyvrészlet Chapter in Book 25 0 Könyv Book 23 0 Egyéb konferenciaközlemény Conference paper 31 0 Egyéb konferenciakötet Conference proceedings 32 0 Oltalmi formák Protection forms 26 0 Disszertáció Thesis 28 0 Egyéb Miscellaneous 29 0 Alkotás Achievement 22 0 Kutatási adat Research data 33 0 Folyóiratcikk Journal Article 24 0 0 0 Könyvrészlet Chapter in Book 25 0 0 0 Könyv Book 23 0 0 0 Egyéb konferenciaközlemény Conference paper 31 0 0 0 Egyéb konferenciakötet Conference proceedings 32 0 0 0 Oltalmi formák Protection forms 26 0 0 0 Disszertáció Thesis 28 0 0 0 Egyéb Miscellaneous 29 0 0 0 Alkotás Achievement 22 0 0 0 Kutatási adat Research data 33 0 0 0 2022 0 2 1 2 1 2023 0 0 0 0 0 2024 0 1 0 1 0 2025 0 1 0 1 0 D1 false false false false 2021-03-28T10:49:52.836+0000 Biokémiai Tanszék (SE / AOK / I / BMBI) 1 16 1 8 0 true Language 10002 /api/language/10002 Angol English true 2 true PersonAuthorship 95065926 /api/authorship/95065926 1 0.125 true false false Author 10021946 /api/author/10021946 Mizsei Réka true 10021946 true Mizsei Réka true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 95065927 /api/authorship/95065927 2 0.125 false false false Li Xiaolong true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 95065928 /api/authorship/95065928 3 0.125 false false false Chen Wan-Na true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 95065929 /api/authorship/95065929 4 0.125 false false false Szabo Monika true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 95065930 /api/authorship/95065930 5 0.125 false false false Wang Jia-Huai true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 95065931 /api/authorship/95065931 6 0.125 false false false Wagner Gerhard true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 95065932 /api/authorship/95065932 7 0.125 false false true Reinherz Ellis L true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 95065933 /api/authorship/95065933 8 0.125 false true true Mallis Robert J true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PublicationIdentifier 18292966 /api/publicationidentifier/18292966 PlainSource 6 /api/publicationsource/6 PublicationSourceType 10001 /api/publicationsourcetype/10001 true true true DOI DOI https://doi.org/@@@ true true 6 true GOLD true IDENTICAL 10.1016/j.jbc.2021.100255 https://doi.org/10.1016/j.jbc.2021.100255 false true PublicationIdentifier 19279819 /api/publicationidentifier/19279819 PlainSource 1 /api/publicationsource/1 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true WoS WoS https://www.webofscience.com/wos/woscc/full-record/@@@ true true 1 true IDENTICAL 000672866400231 https://www.webofscience.com/wos/woscc/full-record/000672866400231 true true PublicationIdentifier 18578362 /api/publicationidentifier/18578362 PlainSource 3 /api/publicationsource/3 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true Scopus Scopus http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-@@@ true true 3 true IDENTICAL 85102946372 http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85102946372 false true PublicationIdentifier 18292965 /api/publicationidentifier/18292965 PlainSource 17 /api/publicationsource/17 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true PubMed PubMed http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=@@@&dopt=Abstract true true 17 true IDENTICAL 33837736 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=33837736&dopt=Abstract false true Classification 10575 /api/classification/10575 true true Keyword 3090 /api/keyword/3090 true 3090 true Keyword 12193 /api/keyword/12193 true 12193 true Keyword 1100836 /api/keyword/1100836 true 1100836 true Keyword 1494385 /api/keyword/1494385 true true Keyword 1532507 /api/keyword/1532507 true true Keyword 2262155 /api/keyword/2262155 true true Keyword 2262156 /api/keyword/2262156 true true Keyword 2262157 /api/keyword/2262157 true true Keyword 2262158 /api/keyword/2262158 true true Keyword 2262159 /api/keyword/2262159 true true SjrRating 11196118 /api/sjrrating/11196118 41 0.1 journal RatingType 10002 /api/ratingtype/10002 sjr true true ClassificationExternal 1303 /api/classificationexternal/1303 true 1303 true D1 DIRECT true true /api/publication/31809540 <div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" mtid="10021946"><a href="/gui2/?type=authors&mode=browse&sel=10021946" target="_blank">Mizsei Réka (<span class="authorship-author-name">Mizsei Réka</span> <span class="authorAux-mtmt"> szerkezeti biokémia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/<span title="Biokémiai és Molekuláris Biológiai Intézet">BMBI</span>/Biokémiai Tanszék</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Li Xiaolong </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Chen Wan-Na </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Szabo Monika </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Wang Jia-Huai </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Wagner Gerhard </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Reinherz Ellis L ✉ </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Mallis Robert J ✉ </span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;31809540" target="_blank">A general chemical crosslinking strategy for structural analyses of weakly interacting proteins applied to preTCR-pMHC complexes</a></div> <div> <span class="journal-title">JOURNAL OF BIOLOGICAL CHEMISTRY</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/0021-9258">0021-9258</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1083-351X">1083-351X</a>)</span>: <span class="journal-volume">296</span> <span class="page"> Paper 100255. 16 p. </span> <span class="year">(2021)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Nyelv: Angol | </span> <span class="identifiers"> <span class="id identifier oa_GOLD" title=" Gold "> <a style="color:blue" title="10.1016/j.jbc.2021.100255" target="_blank" href="https://doi.org/10.1016/j.jbc.2021.100255"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="000672866400231" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/000672866400231"> WoS </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="85102946372" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85102946372"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="33837736" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=33837736&dopt=Abstract"> PubMed </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Folyóirat szakterülete: Scopus - Biochemistry&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;D1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Cell Biology&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Molecular Biology&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q1</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="Nyilvános idézőközlemények összesen, említések nélkül" class="citingPub-count">Nyilvános idéző összesen: 4</span> | Független: 1 | Függő: 3 | Nem jelölt: 0 | WoS jelölt: 4 | WoS/Scopus jelölt:&nbsp;4 | DOI jelölt:&nbsp;4 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;31809540&sort=publishedYear,desc&sort=title"> Idézett közlemények száma: 1 </a> </div> <div class="mtid"><span class="long-pub-mtid">Közlemény: 31809540</span> | <span class="status-data status-VALIDATED"> Egyeztetett </span> Forrás Idéző | <span class="type-subtype">Folyóiratcikk ( Szakcikk ) </span> | <span class="pub-category">Tudományos</span> | <span class="publication-sourceOfData">kézi felvitel</span> </div> <div class="funder"> (Rosztoczy Foundation), Az orvos-, egészségtudományi- és gyógyszerészképzés tudományos műhelyeinek fejlesztése(EFOP-3.6.3-VEKOP-16-2017-00009) Támogató: EFOP-VEKOP, (NTP-NFTÖ-P-15-0693) Támogató: Emberi Erőforrások Minisztériuma, (NTP-NFTÖ-P-17-C-0139) Támogató: Emberi Erőforrások Minisztériuma, (NTP-NFTÖ-P-19-B-0270) Támogató: Emberi Erőforrások Minisztériuma </div> <div class="lastModified">Utolsó módosítás: 2022.06.07. 15:26 Berhidi Anna (SE_KK_Admin5_BA, admin) </div> </div></div>