https://m2.mtmt.hu/ hun 2026-09-21 13:32 JournalArticle 31712141 VALIDATED true Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, United States Department of Conservative Dentistry, Semmelweis University, Budapest, Hungary Export Date: 20 June 2022 CODEN: JDENA Correspondence Address: Levine, M.; Dept of Biochemistry & Molecular Biology, BMSB Rm 802, 940 S.L. Young Blvd, United States; email: martin-levine@ouhsc.edu 0 false 2026-07-09T09:42:02.892+0000 2022-05-30T12:48:16.148+0000 2020-12-07T20:55:29.431+0000 true 10018804 Szalóky-Siki Ágnes /api/admin/10018804 Admin true 10018804 2026-07-09T09:40:53.753+0000 2022-05-30T12:48:16.359+0000 true 10015546 Berhidi Anna /api/admin/10015546 Admin true 10015546 true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Levine, Martin Zinc chloride inhibits lysine decarboxylase production from Eikenella corrodens in vitro and its therapeutic implications true true /api/journal/2417 REVIEWED true false 2417 false 2417 true 0300-5712 1879-176X Journal FOREIGN 104 103533 103533 6 2021 Objectives Dentifrices containing zinc reduce gingival inflammation and bleeding better than control dentifrices (no zinc). How zinc might work is not understood. We have shown that lysine decarboxylase (LdcE), an enzyme from Eikenella corrodens, converts lysine to cadaverine in dental biofilms. The lack of lysine impairs the dentally attached cell barrier to biofilm, causing biofilm products to leak into junctional epithelium and stimulate inflammation. In year-old beagle dogs, immunization with LdcE, induces antibodies that inhibit LdcE activity and retard gingivitis development. We therefore examined whether a zinc-mediated loss of LdcE activity could explain the beneficial effect of zinc dentifrices. Methods We grew E. corrodens in modified tryptic soy broth with or without zinc chloride, and extracted LdcE from the cell surface using a Potter Elvehjem homogenizer. Results Up to 0.96 mM zinc chloride in the bacterial growth medium did not change cell yield, but reduced the extracted protein content by 41 % (R2 = 0.27, p < 0.05) and LdcE activity/mg extracted protein by 85 % (R2 = 0.90, p < 0.001). In extracts from cells grown without zinc, 78 times this zinc chloride concentration (73 mM) was required to reduce LdcE activity by 75 %. Conclusions Zinc ions inhibit the production of protein with LdcE activity at E. corrodens cell surfaces. The zinc ions may attach to cysteine residues that are unique to the N-terminal region of LdcE by interfering with the non-covalent polypeptide assembly that produces enzyme activity. Clinical significance Zinc ion-mediated inhibition of LdcE assembly may provide a rationale for the improved control of gingival inflammation by zinc dentifrices. Funding 2014369 /api/funding/2014369 false true Funding 2014370 /api/funding/2014370 false true Funding 2014371 /api/funding/2014371 false true true 2020 true true true false true false GOLD 2026-07-09 true https://doi.org/10.1016/j.jdent.2020.103533 5 0 5 2 3 5 5 4 4 5 5 2 2 2 3 0 5 2 5 2 0 3 3 Folyóiratcikk Journal Article 24 4 Könyvrészlet Chapter in Book 25 1 Könyv Book 23 0 Egyéb konferenciaközlemény Conference paper 31 0 Egyéb konferenciakötet Conference proceedings 32 0 Oltalmi formák Protection forms 26 0 Disszertáció Thesis 28 0 Egyéb Miscellaneous 29 0 Alkotás Achievement 22 0 Kutatási adat Research data 33 0 Folyóiratcikk Journal Article 24 0 0 0 Könyvrészlet Chapter in Book 25 0 0 0 Könyv Book 23 0 0 0 Egyéb konferenciaközlemény Conference paper 31 0 0 0 Egyéb konferenciakötet Conference proceedings 32 0 0 0 Oltalmi formák Protection forms 26 0 0 0 Disszertáció Thesis 28 0 0 0 Egyéb Miscellaneous 29 0 0 0 Alkotás Achievement 22 0 0 0 Kutatási adat Research data 33 0 0 0 2021 0 1 0 1 0 2022 0 1 1 1 1 2023 0 1 0 1 0 2024 0 1 1 1 1 2025 0 0 0 0 0 2026 0 1 0 1 0 D1 false false false false 2021-03-07T20:57:18.849+0000 Konzerváló Fogászati Klinika (SE / FOK) 1 4 1 3 0 true Language 10002 /api/language/10002 Angol English true 2 true PersonAuthorship 94341910 /api/authorship/94341910 1 0.33333334 true false true Levine Martin true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 94341911 /api/authorship/94341911 2 0.33333334 false false false Collins Lindsay M true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 94341912 /api/authorship/94341912 3 0.33333334 false true false Author 10018912 /api/author/10018912 Lohinai Zsolt true 10018912 true Lohinai Zsolt true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PublicationIdentifier 18018365 /api/publicationidentifier/18018365 PlainSource 6 /api/publicationsource/6 PublicationSourceType 10001 /api/publicationsourcetype/10001 true true true DOI DOI https://doi.org/@@@ true true 6 true GOLD true IDENTICAL 10.1016/j.jdent.2020.103533 https://doi.org/10.1016/j.jdent.2020.103533 false true PublicationIdentifier 18582291 /api/publicationidentifier/18582291 PlainSource 1 /api/publicationsource/1 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true WoS WoS https://www.webofscience.com/wos/woscc/full-record/@@@ true true 1 true IDENTICAL 000619736100013 https://www.webofscience.com/wos/woscc/full-record/000619736100013 false true PublicationIdentifier 21009782 /api/publicationidentifier/21009782 SwordSource 84 /api/publicationsource/84 PublicationSourceType 10007 /api/publicationsourcetype/10007 true true true SE Repozitórium SE Repozitórium https://repo.lib.semmelweis.hu/handle/123456789/@@@ true true 84 true GREEN true IDENTICAL 9096 https://repo.lib.semmelweis.hu/handle/123456789/9096 false true PublicationIdentifier 18252229 /api/publicationidentifier/18252229 PlainSource 3 /api/publicationsource/3 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true Scopus Scopus http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-@@@ true true 3 true IDENTICAL 85097231987 http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85097231987 false true PublicationIdentifier 18582292 /api/publicationidentifier/18582292 PlainSource 17 /api/publicationsource/17 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true PubMed PubMed http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=@@@&dopt=Abstract true true 17 true IDENTICAL 33189800 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=33189800&dopt=Abstract false true Classification 12125 /api/classification/12125 true true Classification 12215 /api/classification/12215 true true Keyword 4815 /api/keyword/4815 true 4815 true Keyword 1001487 /api/keyword/1001487 true 1001487 true Keyword 1004576 /api/keyword/1004576 true 1004576 true Keyword 1598235 /api/keyword/1598235 true true Keyword 1722061 /api/keyword/1722061 true true Keyword 1756742 /api/keyword/1756742 true true SjrRating 11253876 /api/sjrrating/11253876 10 0.1 journal RatingType 10002 /api/ratingtype/10002 sjr true true ClassificationExternal 3501 /api/classificationexternal/3501 true 3501 true D1 DIRECT true true /api/publication/31712141 <div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" >Levine Martin ✉ </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Collins Lindsay M </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10018912"><a href="/gui2/?type=authors&mode=browse&sel=10018912" target="_blank">Lohinai Zsolt (<span class="authorship-author-name">Lohinai Zsolt</span> <span class="authorAux-mtmt"> Konzerváló fogászat</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Fogorvostudományi Kar">FOK</span>/Konzerváló Fogászati Klinika</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;31712141" target="_blank">Zinc chloride inhibits lysine decarboxylase production from Eikenella corrodens in vitro and its therapeutic implications</a></div> <div> <span class="journal-title">JOURNAL OF DENTISTRY</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/0300-5712">0300-5712</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1879-176X">1879-176X</a>)</span>: <span class="journal-volume">104</span> <span class="page"> Paper 103533. 6 p. </span> <span class="year">(2021)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Nyelv: Angol | </span> <span class="identifiers"> <span class="id identifier oa_GOLD" title=" Gold "> <a style="color:blue" title="10.1016/j.jdent.2020.103533" target="_blank" href="https://doi.org/10.1016/j.jdent.2020.103533"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="000619736100013" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/000619736100013"> WoS </a> </span> <span class="id identifier oa_GREEN" title=" Green "> <a style="color:blue" title="9096" target="_blank" href="https://repo.lib.semmelweis.hu/handle/123456789/9096"> SE Repozitórium </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="85097231987" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85097231987"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="33189800" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=33189800&dopt=Abstract"> PubMed </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Folyóirat szakterülete: Scopus - Dentistry (miscellaneous)&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;D1</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="Nyilvános idézőközlemények összesen, említések nélkül" class="citingPub-count">Nyilvános idéző összesen: 5</span> | Független: 2 | Függő: 3 | Nem jelölt: 0 | WoS jelölt: 4 | Scopus jelölt:&nbsp;4 | WoS/Scopus jelölt:&nbsp;5 | DOI jelölt:&nbsp;5 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;31712141&sort=publishedYear,desc&sort=title"> Idézett közlemények száma: 3 </a> </div> <div class="mtid"><span class="long-pub-mtid">Közlemény: 31712141</span> | <span class="status-data status-VALIDATED"> Egyeztetett </span> Forrás Idéző | <span class="type-subtype">Folyóiratcikk ( Szakcikk ) </span> | <span class="pub-category">Tudományos</span> | <span class="publication-sourceOfData">RIS-Egyéb</span> </div> <div class="funder"> EMBERI ERŐFORRÁS FEJLESZTÉSI OPERATÍV PROGRAM (EFOP)(EFOP-3.6.2-162017-00006) Támogató: NKFIH, (Higher Education Excellence Program of the Hungarian Ministry of Human Capacities to Semmelweis University, Therapy Research Module), (KFI_16-1-2017-0409) </div> <div class="lastModified">Utolsó módosítás: 2022.05.30. 14:48 Berhidi Anna (SE_KK_Admin5_BA, admin) </div> <pre class="comment" style="margin-top: 0; margin-bottom: 0;"><u>Megjegyzés</u>: Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, United States Department of Conservative Dentistry, Semmelweis University, Budapest, Hungary Export Date: 20 June 2022 CODEN: JDENA Correspondence Address: Levine, M.; Dept of Bio...</pre> </div></div>