Toxicological analysis of satratoxins, the main toxins in the mushroomTrichoderma cornu-damae, in human serum and mushroom samples by liquid chromatography-tandem mass spectrometry

Ohta, Hikoto ✉; Watanabe, Daisuke; Nomura, Chie; Saito, Daichi; Inoue, Koichi; Miyaguchi, Hajime; Harada, Shuichi; Aita, Yuji

Angol nyelvű Tudományos Szakcikk (Folyóiratcikk)
  • SJR Scopus - Biochemistry (medical): Q2
    Purpose Many poisoning cases involving the deadly toxic mushroomTrichoderma cornu-damaehave been reported, but there are very few reports on toxicological analysis of the poisoning. In this study, a simple and sensitive method was developed for detecting and quantifying satratoxins, which are the main toxins found inT. cornu-damae, in human serum and mushroom samples. Methods The four main toxins, namely, satratoxin H and its 12 '-acetate, 13 '-acetate and 12 ',13 '-diacetate, were isolated fromT. cornu-damaeand used as analytical standards. These standards were spiked into human serum and effective methods were developed for extraction and detection/quantification using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Quantification of satratoxins inT. cornu-damaesamples was performed by the standard addition method. Results Although satratoxins, which have neutral terpene structures, showed very low sensitivities in conventional LC-MS/MS analysis, they could be detected with enough sensitivity by our developed method. In human serum, the limit of detection was 0.1 ng/mL and the limit of quantification was 1 ng/mL for all four satratoxins. The recovery rate ranged from 70.5 to 86.6%, and the coefficients of determination for calibration curves were > 0.999. Satratoxins inT. cornu-damaesamples were also well detected and quantified with coefficients of determination for calibration curves of > 0.997 and intraday/interday precision (relative standard deviation) ranging from 2.98 to 10.3%. Conclusions To our knowledge, this is the first report of toxicological analysis of satratoxins using analytical standards.
    Hivatkozás stílusok: IEEEACMAPAChicagoHarvardCSLMásolásNyomtatás
    2021-05-17 15:00