Purpose Many poisoning cases involving the deadly toxic mushroomTrichoderma cornu-damaehave
been reported, but there are very few reports on toxicological analysis of the poisoning.
In this study, a simple and sensitive method was developed for detecting and quantifying
satratoxins, which are the main toxins found inT. cornu-damae, in human serum and
mushroom samples. Methods The four main toxins, namely, satratoxin H and its 12 '-acetate,
13 '-acetate and 12 ',13 '-diacetate, were isolated fromT. cornu-damaeand used as
analytical standards. These standards were spiked into human serum and effective methods
were developed for extraction and detection/quantification using liquid chromatography-tandem
mass spectrometry (LC-MS/MS). Quantification of satratoxins inT. cornu-damaesamples
was performed by the standard addition method. Results Although satratoxins, which
have neutral terpene structures, showed very low sensitivities in conventional LC-MS/MS
analysis, they could be detected with enough sensitivity by our developed method.
In human serum, the limit of detection was 0.1 ng/mL and the limit of quantification
was 1 ng/mL for all four satratoxins. The recovery rate ranged from 70.5 to 86.6%,
and the coefficients of determination for calibration curves were > 0.999. Satratoxins
inT. cornu-damaesamples were also well detected and quantified with coefficients of
determination for calibration curves of > 0.997 and intraday/interday precision (relative
standard deviation) ranging from 2.98 to 10.3%. Conclusions To our knowledge, this
is the first report of toxicological analysis of satratoxins using analytical standards.