https://m2.mtmt.hu/ eng 2026-10-23 04:50 JournalArticle 31675268 VALIDATED true Funding Agency and Grant Number: Hungarian Human Resources Development Operational Program [EFOP-3.6.2-16-2017-00006]; Thematic Excellence Programme of the Ministry for Innovation and Technology in Hungary [2020-4.1.1.-TKP2020] Funding text: This work was supported by the Hungarian Human Resources Development Operational Program (EFOP-3.6.2-16-2017-00006). Additional support was received from the Thematic Excellence Programme (2020-4.1.1.-TKP2020) of the Ministry for Innovation and Technology in Hungary, within the framework of the Therapy thematic programme of the Semmelweis University. This article is dedicated to the memory of Prof. Gyorgy Simon. 0 false 2026-10-20T22:12:58.963+0000 2022-05-26T13:34:08.040+0000 2020-11-27T19:31:22.921+0000 true 10018804 Szalóky-Siki Ágnes /api/admin/10018804 Admin true 10018804 2025-03-06T10:22:15.993+0000 2022-04-01T08:19:14.864+0000 true 10062062 Gere Tamásné /api/admin/10062062 Admin true 10062062 true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Perczel-Kovách, Katalin STRO-1 positive cell expansion during osteogenic differentiation: a comparative study of three mesenchymal stem cell types of dental origin true true /api/journal/515 REVIEWED true false 515 false 515 true 0003-9969 1879-1506 Journal FOREIGN 122 104995 104995 11 2021 Objective Although the osteogenic differentiation potential of mesenchymal stem cells of dental origin is well established, the roles of different marker proteins in this process remain to be clarified. Our aim was to compare the cellular and molecular changes, focusing in particular on mesenchymal stem cell markers, during in vitro osteogenesis in three dental stem cell types: dental follicle stem cells (DFSCs), periodontal ligament stem cells (PDLSCs) and dental pulp stem cells (DPSCs). Design Human DFSCs, PDLSCs and DPSCs were isolated, cultured and their osteogenic differentiation was induced for 3 weeks. Mineralization was assessed by von Kossa staining and calcium concentration measurements. The expression of mesenchymal and osteogenic markers was studied by immunocytochemistry and qPCR techniques. Alkaline phosphatase (ALP) activity and the frequency of STRO-1 positive cells were also quantified. Results The three cultures all showed abundant mineralization, with high calcium content by day 21. The expression of vimentin and nestin was sustained after osteogenic induction. The osteogenic medium induced a considerable elevation of STRO-1 positive cells. By day 7, the ALP mRNA level had increased more than 100-fold in DFSCs, PDLSCs, and DPSCs. Quantitative PCR results indicated dissimilarities of osteoblastic marker levels in the three dental stem cell cultures. Conclusions DFSCs, PDLSCs and DPSCs have similar functional osteogenic differentiation capacities although their expressional profiles of key osteogenic markers show considerable variations. The STRO-1 positive cell fraction expands during osteogenic differentiation while vimentin and nestin expression remain high. For identification of stemness, functional studies rather than marker expressions are needed. 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Nagy Krisztina true 10021907 true Nagy Krisztina S. true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PublicationIdentifier 17902828 /api/publicationidentifier/17902828 PlainSource 6 /api/publicationsource/6 PublicationSourceType 10001 /api/publicationsourcetype/10001 true true true DOI DOI https://doi.org/@@@ true true 6 true PAY IDENTICAL 10.1016/j.archoralbio.2020.104995 https://doi.org/10.1016/j.archoralbio.2020.104995 false true PublicationIdentifier 18464696 /api/publicationidentifier/18464696 PlainSource 1 /api/publicationsource/1 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true WoS WoS https://www.webofscience.com/wos/woscc/full-record/@@@ true true 1 true IDENTICAL 000608756100011 https://www.webofscience.com/wos/woscc/full-record/000608756100011 true true PublicationIdentifier 20324243 /api/publicationidentifier/20324243 SwordSource 84 /api/publicationsource/84 PublicationSourceType 10007 /api/publicationsourcetype/10007 true true true SE Repozitórium SE Repozitórium https://repo.lib.semmelweis.hu/handle/123456789/@@@ true true 84 true RESTRICTED IDENTICAL 8988 https://repo.lib.semmelweis.hu/handle/123456789/8988 false true PublicationIdentifier 18124427 /api/publicationidentifier/18124427 PlainSource 3 /api/publicationsource/3 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true Scopus Scopus http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-@@@ true true 3 true IDENTICAL 85096971236 http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85096971236 true true PublicationIdentifier 18464724 /api/publicationidentifier/18464724 PlainSource 17 /api/publicationsource/17 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true PubMed PubMed http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=@@@&dopt=Abstract true true 17 true IDENTICAL 33278647 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=33278647&dopt=Abstract true true Classification 12215 /api/classification/12215 true true Keyword 1008903 /api/keyword/1008903 true 1008903 true Keyword 1027014 /api/keyword/1027014 true 1027014 true Keyword 1027407 /api/keyword/1027407 true 1027407 true Keyword 1030392 /api/keyword/1030392 true 1030392 true Keyword 1038037 /api/keyword/1038037 true 1038037 true Keyword 1501538 /api/keyword/1501538 true true Keyword 1601075 /api/keyword/1601075 true true Keyword 1800674 /api/keyword/1800674 true true SjrRating 11253912 /api/sjrrating/11253912 44 0.49 journal RatingType 10002 /api/ratingtype/10002 sjr true true ClassificationExternal 3501 /api/classificationexternal/3501 true 3501 true Q2 DIRECT true true /api/publication/31675268 <div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" mtid="10045264"><a href="/gui2/?type=authors&mode=browse&sel=10045264" target="_blank">Perczel-Kovách Katalin (<span class="authorship-author-name">Perczel-Kovách Katalin Erzsébet</span> <span class="authorAux-mtmt"> orálbiológia, fogeredetű őssejtek</span>) </a> </span> <span class="author-affil"><span title="Semmelweis University">SU</span>/<span title="Faculty of Dentistry">FD</span>/Department of Oral Biology</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10064249"><a href="/gui2/?type=authors&mode=browse&sel=10064249" target="_blank">Hegedűs Orsolya (<span class="authorship-author-name">Hegedűs Orsolya</span> <span class="authorAux-mtmt"> Fogorvostudományok</span>) </a> </span> <span class="author-affil"><span title="Semmelweis University">SU</span>/<span title="Faculty of Dentistry">FD</span>/Department of Oral Biology</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="105"><a href="/gui2/?type=authors&mode=browse&sel=105" target="_blank">Földes Anna (<span class="authorship-author-name">Földes Anna</span> <span class="authorAux-mtmt"> Molekulár biológia, Élettan</span>) </a> </span> <span class="author-affil"><span title="Semmelweis University">SU</span>/<span title="Faculty of Dentistry">FD</span>/Department of Oral Biology</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10064955"><a href="/gui2/?type=authors&mode=browse&sel=10064955" target="_blank">Sangngoen Thanyaporn (<span class="authorship-author-name">Thanyaporn Sang-ngoen</span> <span class="authorAux-mtmt"> orálbiológia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis University">SU</span>/<span title="Faculty of Dentistry">FD</span>/Department of Oral Biology</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10018992"><a href="/gui2/?type=authors&mode=browse&sel=10018992" target="_blank">Kálló Karola (<span class="authorship-author-name">Kálló Karola</span> <span class="authorAux-mtmt"> Orálbiológia, embertan</span>) </a> </span> <span class="author-affil"><span title="Semmelweis University">SU</span>/<span title="Faculty of Dentistry">FD</span>/Department of Oral Biology</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10073730"><a href="/gui2/?type=authors&mode=browse&sel=10073730" target="_blank">Steward Martin C. (<span class="authorship-author-name">Steward Martin Charles</span> <span class="authorAux-mtmt"> Élettan</span>) </a> </span> <span class="author-affil"><span title="Semmelweis University">SU</span>/<span title="Faculty of Dentistry">FD</span>/Department of Oral Biology</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10006652"><a href="/gui2/?type=authors&mode=browse&sel=10006652" target="_blank">Varga Gábor ✉ (<span class="authorship-author-name">Varga Gábor</span> <span class="authorAux-mtmt"> Élettan, orálbiológia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis University">SU</span>/<span title="Faculty of Dentistry">FD</span>/Department of Oral Biology</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10021907"><a href="/gui2/?type=authors&mode=browse&sel=10021907" target="_blank">Nagy Krisztina S. ✉ (<span class="authorship-author-name">S. Nagy Krisztina</span> <span class="authorAux-mtmt"> Sejtbiológia, őssejtek, tumorsejtek</span>) </a> </span> <span class="author-affil"><span title="Semmelweis University">SU</span>/<span title="Faculty of Dentistry">FD</span>/Department of Oral Biology</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;31675268" target="_blank">STRO-1 positive cell expansion during osteogenic differentiation: a comparative study of three mesenchymal stem cell types of dental origin</a></div> <div> <span class="journal-title">ARCHIVES OF ORAL BIOLOGY</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/0003-9969">0003-9969</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1879-1506">1879-1506</a>)</span>: <span class="journal-volume">122</span> <span class="page"> Paper 104995. 11 p. </span> <span class="year">(2021)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Language: English | </span> <span class="identifiers"> <span class="id identifier oa_PAY" title=" Pay "> <a style="color:blue" title="10.1016/j.archoralbio.2020.104995" target="_blank" href="https://doi.org/10.1016/j.archoralbio.2020.104995"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="000608756100011" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/000608756100011"> WoS </a> </span> <span class="id identifier oa_RESTRICTED" title=" Restricted "> <a style="color:blue" title="8988" target="_blank" href="https://repo.lib.semmelweis.hu/handle/123456789/8988"> SE Repozitórium </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="85096971236" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85096971236"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="33278647" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=33278647&dopt=Abstract"> PubMed </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Journal subject: Scopus - Dentistry (miscellaneous)&nbsp;&nbsp;&nbsp;Rank:&nbsp;Q2</div> <div class="journal-subject">Journal subject: Scopus - Medicine (miscellaneous)&nbsp;&nbsp;&nbsp;Rank:&nbsp;Q2</div> <div class="journal-subject">Journal subject: Scopus - Otorhinolaryngology&nbsp;&nbsp;&nbsp;Rank:&nbsp;Q2</div> <div class="journal-subject">Journal subject: Scopus - Cell Biology&nbsp;&nbsp;&nbsp;Rank:&nbsp;Q3</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="" class="citingPub-count">Citing papers: 25</span> | Independent citation: 22 | Self citation: 3 | Unknown citation: 0 | Number of citations in WoS: 23 | Number of citations in Scopus:&nbsp;23 | WoS/Scopus assigned:&nbsp;24 | Number of citations with DOI:&nbsp;24 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;31675268&sort=publishedYear,desc&sort=title"> Number of cited publications: 4 </a> </div> <div class="mtid"><span class="long-pub-mtid">Publication: 31675268</span> | <span class="status-data status-VALIDATED"> Validated </span> Core Citing | <span class="type-subtype">Journal Article ( Article ) </span> | <span class="pub-category">Scientific</span> | <span class="publication-sourceOfData">RIS-Egyéb</span> </div> <div class="funder"> EMBERI ERŐFORRÁS FEJLESZTÉSI OPERATÍV PROGRAM (EFOP)(EFOP-3.6.2-162017-00006) Funder: NRDIO, Thematic Excellence Program (Semmelweis University)(2020-4.1.1.-TKP2020) Funder: Ministry for Innovation and Technology </div> <div class="lastModified">Last Modified: 2022.05.26. 15:34 Anna Berhidi (SE_KK_Admin5_BA, admin) </div> <pre class="comment" style="margin-top: 0; margin-bottom: 0;"><u>Comments</u>: Funding Agency and Grant Number: Hungarian Human Resources Development Operational Program [EFOP-3.6.2-16-2017-00006]; Thematic Excellence Programme of the Ministry for Innovation and Technology in Hungary [2020-4.1.1.-TKP2020] Funding text: This work was supported by the Hungarian Human Resources Development Operational Program (EFOP-3.6.2-16-2017-00006). Additional support was rec...</pre> </div></div>