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"Gyorgy", "givenName" : "Panyi", "authorTyped" : true, "editorTyped" : false, "otherTyped" : false, "type" : { "otype" : "AuthorshipType", "mtid" : 1, "link" : "/api/authorshiptype/1", "label" : "Szerző", "code" : 0, "published" : true, "oldId" : 0, "snippet" : true }, "published" : false, "snippet" : true } ], "title" : "Ion channel gene transcripts in chorion-derived mesenchymal stem cells", "internalId" : "9789633067642", "firstPageOrInternalIdForSort" : "9789633067642", "publishedYear" : 2020, "abstractText" : "Introduction: \nMesenchymal stem cells (MSCs) have gained interest for regenerative medicine. Several studies suggested the attractive therapeutic potential of the placenta-derived MSC, however, literature is insufficient regarding the expression of ion channels in chorion/placenta-derived MSCs (cMSCs). Since ion channels are known to regulate many cell functions in non-excitable cells as well, our aim was to study the expression of ion channels in cMSCs.\nMethods:\nThe heterogeneity of cMSC culture is a challenge when studying ion channels. We designed a cell sorting system using multicolor flow cytometry which can distinguish MSCs from other cell types within cMSCs culture. We analysed the mRNA expression of different cell type markers and ion channels using qPCR.\nResults:\nmRNA markers of fibroblast, syncytiotrophoblasts, smooth muscle and endothelial cells were detected in cMSCs. We designed a cell sorting system using the combination of fluorophore-conjugated anti-CD73, -CD90, -CD34, - CD45 antibodies. Sorted cMSCs also expressed GATA, Nanog, Oct4, Sox2 stem cell markers. We detected the mRNA of Kv1.3, Kv4.2, Kv4.3, and Kv10.1, KCa1.1 associated with β1-, β2a-, β3b, c, d-subunits, Nav1.2, Nav1.7, CLCN2-4 and CLCN6-7 channels in sorted and non-sorted cMSCs. \nConclusions:\nMultiple ion channel mRNAs were detected in cMSCs. The mRNA phenotypes of sorted and non-sorted cMSCs, were similar, however, this finding does not exclude qualitative and quantitative differences in the expression at protein level. Cell sorting is recommended for single cell electrophysiology methods aimed at identifying the functional expression of the channels. \nAcknowledgements: This work was supported by EFOP 3.6.2-16-2017-00006 (LIVE LONGER).", "fundings" : [ { "otype" : "Funding", "mtid" : 1991032, "link" : "/api/funding/1991032", "label" : "Modern orvostudományi diagnosztikus eljárások és terápiák fejlesztése transzlációs megközelítésbe...(EFOP-3.6.2-16-2017-00006) Támogató: EFOP", "published" : false, "snippet" : true } ], "digital" : null, "printed" : null, "sourceYear" : 2020, "foreignEdition" : false, "foreignLanguage" : true, "fullPublication" : false, "conferencePublication" : false, "nationalOrigin" : null, "missingAuthor" : false, "oaType" : "NONE", "oaCheckDate" : "2023-01-24", "oaFree" : false, "citationCount" : 0, "citationCountUnpublished" : 0, "citationCountWoOther" : 0, "independentCitCountWoOther" : 0, "nationalOriginCitationCount" : 0, "foreignEditionCitationCount" : 0, "doiCitationCount" : 0, "wosCitationCount" : 0, "scopusCitationCount" : 0, "wosScopusCitationCount" : 0, "wosScopusCitationCountWoOther" : 0, "wosScopusIndependentCitationCount" : 0, "wosScopusIndependentCitationCountWoOther" : 0, "independentCitationCount" : 0, "selfCitationCount" : 0, "unhandledCitationCount" : 0, "citingPubCount" : 0, "independentCitingPubCount" : 0, "citingPubCountWoOther" : 0, "independentCitingPubCountWoOther" : 0, "unhandledCitingPubCount" : 0, "citedPubCount" : 0, "citedCount" : 0, "hasCitationDuplums" : false, "userChangeableUntil" : "2021-02-23T13:00:44.874+0000", "directInstitutesForSort" : "Biofizikai Tanszék (DE / ÁOK / BSI); Biofizikai és Sejtbiológiai Intézet (DE / ÁOK); MTA-DE Sejtbiológiai és Jelátviteli Kutatócsoport (DE / ÁOK)", "ownerAuthorCount" : 5, "ownerInstituteCount" : 15, "directInstituteCount" : 3, "authorCount" : 5, "contributorCount" : 0, "book" : { "otype" : "Book", "mtid" : 31636098, "link" : "/api/publication/31636098", "label" : "Rakonczay Zoltán. 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