Development of malignant tumors is preceded by molecular biological events. Our aim
was to establish an assay panel by using miRNAs and other genes for the rapid screening
of potential carcinogens or chemopreventive agents.Six male and 6 female CBA/Ca mice
received 20 mg/bwkg 7,12-dimethylbenz(α)anthracene (DMBA) intraperitoneally, and 24
h later RNA was isolated from parenchymal organs. Expression of miR-330, miR-29a,
miR-9-1, miR-9-3 and mTORC1 was analysed by real time polymerase chain reaction and
compared to non-treated controls.DMBA caused significant alterations in the expression
of the studied genes. The most profound changes were the strongly elevated miR-9-3
and mTORC1 expressions in female mice in all organs studied.miR-9-3 and mTORC1 expression
in female mice were found to be the most suitable biomarkers for rapid identification
of possible carcinogenic effects.
