https://m2.mtmt.hu/ eng 2026-10-25 00:19 JournalArticle 3150353 APPROVED true Department of Neuroscience, Physiology and Pharmacology, University College London, London, United Kingdom Laboratory of Cellular Neurophysiology, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, Göttingen, Germany Team Synapse in Cognition, Interdisciplinary Institute for Neuroscience, Universitéde Bordeaux, UMR 5297, Bordeaux, F-33000, France Cited By :34 Export Date: 13 June 2024 Correspondence Address: Nusser, Z.; Laboratory of Cellular Neurophysiology, Hungary; email: nusser@koki.hu Chemicals/CAS: cytochalasin D, 22144-77-0; jaspamide, 102396-24-7; latrunculin B, 76343-94-7; okadaic acid, 78111-17-8; roscovitine, 186692-46-6; ubidecarenone, 303-98-0 Funding details: Seventh Framework Programme, FP7, 293681, 294667 Funding details: Seventh Framework Programme, FP7 0 2018-02-20T17:30:13.000+0000 false 3150353 2026-10-17T23:17:35.892+0000 2024-01-08T18:48:57.704+0000 2016-12-09T08:35:25.000+0000 true 10000499 Nusser Zoltán /api/author/10000499 Author true 10000499 2025-03-17T08:33:14.463+0000 2025-03-17T08:33:14.463+0000 true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Rothman, JS Physical determinants of vesicle mobility and supply at a central synapse. true true /api/journal/10031024 REVIEWED true false 10031024 false 10031024 true 2050-084X Journal FOREIGN 5 e15133 e15133 30 2016 Encoding continuous sensory variables requires sustained synaptic signalling. At several sensory synapses, rapid vesicle supply is achieved via highly mobile vesicles and specialized ribbon structures, but how this is achieved at central synapses without ribbons is unclear. Here we examine vesicle mobility at excitatory cerebellar mossy fibre synapses which sustain transmission over a broad frequency bandwidth. Fluorescent recovery after photobleaching in slices from VGLUT1(Venus) knock-in mice reveal 75% of VGLUT1-containing vesicles have a high mobility, comparable to that at ribbon synapses. Experimentally constrained models establish hydrodynamic interactions and vesicle collisions are major determinants of vesicle mobility in crowded presynaptic terminals. Moreover, models incorporating 3D reconstructions of vesicle clouds near active zones (AZs) predict the measured releasable pool size and replenishment rate from the reserve pool. They also show that while vesicle reloading at AZs is not diffusion-limited at the onset of release, diffusion limits vesicle reloading during sustained high-frequency signalling. Funding 2005682 /api/funding/2005682 false true Funding 2005683 /api/funding/2005683 false true true true 2016 789298 true true true false true false GOLD 2026-10-18 true https://doi.org/10.7554/eLife.15133 true 10030885 Bálint Eszter /api/admin/10030885 Admin true 10030885 40 0 40 36 2 40 40 39 39 40 40 36 36 36 4 0 40 36 40 36 0 2 2 Folyóiratcikk Journal Article 24 40 Könyvrészlet Chapter in Book 25 0 Könyv Book 23 0 Egyéb konferenciaközlemény Conference paper 31 0 Egyéb konferenciakötet Conference proceedings 32 0 Oltalmi formák Protection forms 26 0 Disszertáció Thesis 28 0 Egyéb Miscellaneous 29 0 Alkotás Achievement 22 0 Kutatási adat Research data 33 0 Folyóiratcikk Journal Article 24 0 0 0 Könyvrészlet Chapter in Book 25 0 0 0 Könyv Book 23 0 0 0 Egyéb konferenciaközlemény Conference paper 31 0 0 0 Egyéb konferenciakötet Conference proceedings 32 0 0 0 Oltalmi formák Protection forms 26 0 0 0 Disszertáció Thesis 28 0 0 0 Egyéb Miscellaneous 29 0 0 0 Alkotás Achievement 22 0 0 0 Kutatási adat Research data 33 0 0 0 2016 0 2 2 2 2 2017 0 5 5 5 5 2018 0 5 4 5 4 2019 0 3 2 3 2 2020 0 5 5 5 5 2021 0 4 4 4 4 2022 0 3 3 3 3 2023 0 5 4 5 4 2024 0 5 5 5 5 2025 0 2 2 2 2 2026 0 1 0 1 0 D1 false false false false 2017-05-02T06:07:31.000+0000 2017-02-01T07:07:31.000+0000 Celluláris Idegélettani Kutatócsoport (HRN KOKI) 1 6 1 5 0 true Language 10002 /api/language/10002 Angol English true 2 true PersonAuthorship 8599698 /api/authorship/8599698 1 0.2 true false Rothman JS true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 25382256 true PersonAuthorship 8599699 /api/authorship/8599699 2 0.2 false false Kocsis L true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 25382255 true PersonAuthorship 8599700 /api/authorship/8599700 3 0.2 false false Herzog E true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 25382254 true PersonAuthorship 8599701 /api/authorship/8599701 4 0.2 false false true Author 10000499 /api/author/10000499 Nusser Zoltán true 10000499 true Nusser Z true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 25382253 true PersonAuthorship 8599702 /api/authorship/8599702 5 0.2 false true true Silver RA true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 25382252 true PublicationIdentifier 1151926 /api/publicationidentifier/1151926 PlainSource 6 /api/publicationsource/6 PublicationSourceType 10001 /api/publicationsourcetype/10001 true true true DOI DOI https://doi.org/@@@ true true 6 true GOLD true IDENTICAL 10.7554/eLife.15133 https://doi.org/10.7554/eLife.15133 false 1503373 true PublicationIdentifier 11759362 /api/publicationidentifier/11759362 PlainSource 1 /api/publicationsource/1 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true WoS WoS https://www.webofscience.com/wos/woscc/full-record/@@@ true true 1 true IDENTICAL 000384260300001 https://www.webofscience.com/wos/woscc/full-record/000384260300001 false 13722605 true PublicationIdentifier 1151925 /api/publicationidentifier/1151925 PlainSource 3 /api/publicationsource/3 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true Scopus Scopus http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-@@@ true true 3 true false IDENTICAL 84988476345 http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-84988476345 false 1725954 true PublicationIdentifier 1151927 /api/publicationidentifier/1151927 PlainSource 17 /api/publicationsource/17 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true PubMed PubMed http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=@@@&dopt=Abstract true true 17 true false NO 27542193 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=27542193&dopt=Abstract false 1503374 true Classification 12125 /api/classification/12125 true true Classification 10545 /api/classification/10545 true true Classification 12548 /api/classification/12548 true true Classification 12215 /api/classification/12215 true true SjrRating 10946621 /api/sjrrating/10946621 21 0.0 journal RatingType 10002 /api/ratingtype/10002 sjr true true ClassificationExternal 2701 /api/classificationexternal/2701 true 2701 true D1 DIRECT true true /api/publication/3150353 <div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" >Rothman JS </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Kocsis L </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Herzog E </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10000499"><a href="/gui2/?type=authors&mode=browse&sel=10000499" target="_blank">Nusser Z ✉ (<span class="authorship-author-name">Nusser Zoltán</span> <span class="authorAux-mtmt"> Celluláris idegélettan</span>) </a> </span> <span class="author-affil"><span title="MTA Kísérleti Orvostudományi Kutatóintézet">IEMHAS</span>/<span title="Department of Cellular and Network Neurobiology">DCNN</span>/Laboratory of Cellular Neurophysiology</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Silver RA ✉ </span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;3150353" target="_blank">Physical determinants of vesicle mobility and supply at a central synapse.</a></div> <div> <span class="journal-title">ELIFE</span> <span class="journal-issn">( <a target="_blank" href="https://portal.issn.org/resource/ISSN/2050-084X">2050-084X</a>)</span>: <span class="journal-volume">5</span> <span class="page"> Paper e15133. 30 p. </span> <span class="year">(2016)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Language: English | </span> <span class="identifiers"> <span class="id identifier oa_GOLD" title=" Gold "> <a style="color:blue" title="10.7554/eLife.15133" target="_blank" href="https://doi.org/10.7554/eLife.15133"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="000384260300001" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/000384260300001"> WoS </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="84988476345" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-84988476345"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:black" title="27542193" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=27542193&dopt=Abstract"> PubMed </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Journal subject: Scopus - Biochemistry, Genetics and Molecular Biology (miscellaneous)&nbsp;&nbsp;&nbsp;Rank:&nbsp;D1</div> <div class="journal-subject">Journal subject: Scopus - Medicine (miscellaneous)&nbsp;&nbsp;&nbsp;Rank:&nbsp;D1</div> <div class="journal-subject">Journal subject: Scopus - Neuroscience (miscellaneous)&nbsp;&nbsp;&nbsp;Rank:&nbsp;D1</div> <div class="journal-subject">Journal subject: Scopus - Immunology and Microbiology (miscellaneous)&nbsp;&nbsp;&nbsp;Rank:&nbsp;Q1</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="" class="citingPub-count">Citing papers: 40</span> | Independent citation: 36 | Self citation: 4 | Unknown citation: 0 | Number of citations in WoS: 39 | Number of citations in Scopus:&nbsp;39 | WoS/Scopus assigned:&nbsp;40 | Number of citations with DOI:&nbsp;40 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;3150353&sort=publishedYear,desc&sort=title"> Number of cited publications: 2 </a> </div> <div class="mtid"><span class="long-pub-mtid">Publication: 3150353</span> | <span class="status-data status-APPROVED"> Published </span> <span class="oldId">Old id: 3150353</span> | Core Citing | <span class="type-subtype">Journal Article ( Article ) </span> | <span class="pub-category">Scientific</span> | <span class="publication-sourceOfData">MEDLINE</span> </div> <div class="funder"> Seventh Framework Programme(FP7, 294667), Seventh Framework Programme(FP7 293681) </div> <div class="lastModified">Last Modified: 2024.01.08. 19:48 Márta Turek (KOKI admin 4) </div> <pre class="comment" style="margin-top: 0; margin-bottom: 0;"><u>Comments</u>: Department of Neuroscience, Physiology and Pharmacology, University College London, London, United Kingdom Laboratory of Cellular Neurophysiology, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, Göttingen, Germany ...</pre> </div></div>