Conservation and divergence in NaChBac and Na(V)1.7 pharmacology reveals novel drug interaction mechanisms

Zhu, Wandi ✉; Li, Tianbo; Silva, Jonathan R.; Chen, Jun ✉

Angol nyelvű Tudományos Szakcikk (Folyóiratcikk)
Megjelent: SCIENTIFIC REPORTS 2045-2322 10 (1) Paper: 10730 , 11 p. 2020
  • Szociológiai Tudományos Bizottság: B
  • SJR Scopus - Multidisciplinary: D1
    Voltage-gated Na+ (Na-V) channels regulate homeostasis in bacteria and control membrane electrical excitability in mammals. Compared to their mammalian counterparts, bacterial Na-V channels possess a simpler, fourfold symmetric structure and have facilitated studies of the structural basis of channel gating. However, the pharmacology of bacterial Na-V remains largely unexplored. Here we systematically screened 39 Na-V modulators on a bacterial channel (NaChBac) and characterized a selection of compounds on NaChBac and a mammalian channel (human Na(V)1.7). We found that while many compounds interact with both channels, they exhibit distinct functional effects. For example, the local anesthetics ambroxol and lidocaine block both Na(V)1.7 and NaChBac but affect activation and inactivation of the two channels to different extents. The voltage-sensing domain targeting toxin BDS-I increases Na(V)1.7 but decreases NaChBac peak currents. The pore binding toxins aconitine and veratridine block peak currents of Na(V)1.7 and shift activation (aconitine) and inactivation (veratridine) respectively. In NaChBac, they block the peak current by binding to the pore residue F224. Nonetheless, aconitine has no effect on activation or inactivation, while veratridine only modulates activation of NaChBac. The conservation and divergence in the pharmacology of bacterial and mammalian Na-V channels provide insights into the molecular basis of channel gating and will facilitate organism-specific drug discovery.
    Hivatkozás stílusok: IEEEACMAPAChicagoHarvardCSLMásolásNyomtatás
    2021-01-26 21:42