Antiinflammatory properties of pulsed magnetic field (PMF) treatments or administration
of antiLy6G antibody have been previously reported. In this study, we hypothesized
that, the combination of PMF treatments and antiLy6G administration may synergistically
potentiate their antiinflammatory actions. The effects of the combination of PMF treatments
and antiLy6G administration were investigated by examining the inflammatory signs,
histopathological properties of the inflamed site, and measuring the macrophage inflammatory
protein-1 alpha (MIP-1 alpha/CCL3) and myeloperoxidase (MPO) levels of inflamed paw
tissues in rats with carrageenan-induced acute paw inflammation. In this present study,
PMF treatments alone or administration of antiLy6G alone ameliorated the acute inflammation.
However, their combination exacerbated the inflammatory signs, hyperalgesia, allodynia,
edema and fever, and aggravated the inflammatory conditions by excessive infiltration
of inflammatory cells to the inflamed site. These opposing effects of the combined
treatments may correlate with enhanced levels of MIP-1 alpha and MPO in inflamed paws.
Present results indicated that the combination of the PMF treatments and antiLy6G
administration may not provide additional benefits and may actually cause an aggravation
of the acute inflammatory process. Findings may also suggest that during neutrophil
or immune cell-targeted treatments for inflammatory states, magnetic field exposure
may cause unexpected negative consequences.