https://m2.mtmt.hu/ eng 2026-04-17 02:20 JournalArticle 31313575 VALIDATED true Funding Agency and Grant Number: Hungarian Scientific Research FundOrszagos Tudomanyos Kutatasi Alapprogramok (OTKA) [K112364]; Stipendium Hungaricum Tempus Public Foundation Funding text: Hungarian Scientific Research Fund, Grant/Award Number: K112364; Stipendium Hungaricum Tempus Public Foundation 0 false 2025-12-29T15:18:52.526+0000 2021-09-21T08:09:21.979+0000 2020-05-14T19:27:55.652+0000 true 10018804 Szalóky-Siki Ágnes /api/admin/10018804 Admin true 10018804 2025-02-04T13:52:02.765+0000 2021-01-07T09:50:45.703+0000 true 10019965 Ádám Adrienn /api/admin/10019965 Admin true 10019965 2021-09-21T08:09:21.995+0000 true 10062062 Gere Tamásné /api/admin/10062062 Admin true 10062062 Admin 10014568 /api/admin/10014568 Vándor Andrea true 10014568 true 2023-08-22T13:58:03.074+0000 true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Khorolsuren, Zambaga Functional and cell surface characteristics of periodontal ligament cells (PDLCs) on RGD-synthetic polypeptide conjugate coatings true true /api/journal/2758 REVIEWED true false 2758 false 2758 true 0022-3484 1600-0765 Journal FOREIGN 55 5 713 723 713 11 2020 Abstract Background and Objective Periodontal ligament cells (PDLCs) are an important source for periodontal tissue healing and regeneration. Proper cell adhesion is a key for survival of anchorage-dependent cells and also initiates further intracellular signals for essential cellular functions. We aimed to test 3 different synthetic conjugates with integrin-binding RGD sequence (SAK-c[RGDfC], AK-c[RGDfC], and SAK-opn on the adhesion of human PDLCs and subsequent events including proliferation, migration, behavior of cell surface molecules, and osteogenic differentiation. Materials and Methods Synthetic peptides were synthesized by solid-phase technique and attached to branched chain polymeric polypeptides via thioether linkage. Simple adsorption method was used to coat tissue culture plastic or electric arrays. PDLCs were isolated from 24 surgically extracted human third molars. Cell adhesion and proliferation were measured with real-time impedimetric xCELLigence SP system. Cell migration assay was performed with Ibidi? Culture inserts. Cell surface antigens were detected using flow cytometry analysis. Osteogenic differentiation was assessed with alkaline phosphatase (ALP) assay and Alizarin Red S staining, and real-time qPCR was performed to analyze the osteoblast-related gene expression. Osteogenic differentiation and adipogenic differentiation of PDLCs were monitored by real-time Electrical Cell-Substrate Impedance Spectroscopy (ECIS). Results Primary outcome of this study relies on that all three synthetic RGD peptides improved PDLC adhesion (P < .05). When animal serum is absent in culture medium, SAK-c[RGDfC] and AK-c[RGDfC] elevated cell adhesion (P < .05). Cell migration was enhanced by SAK-c[RGDfC] and AK-c[RGDfC] (P < .05). After 1-week treatment, all synthetic peptides elevated CD105 (1.7- to 2.2-fold) and CD146 (1.3- to 1.5-fold) markers and caused different integrin patterns. ALP activity (1.4-fold) and ARS (1.8- and 2.0-fold) were increased by SAK-c[RGDfC] and AK-c[RGDfC] in absence of osteogenic supplements, and all the peptides supported the mineralization under osteogenic condition (P < .05). RT-qPCR revealed the upregulation of bone sialoprotein (5.0- to 7.8-fold), osteocalcin (2.3- to 2.7-fold), and ALP (1.9- to 2.3-fold) gene expression in osteogenesis-induced PDLCs. ECIS monitoring showed that higher impedance was generated by the osteogenic induction compared with the adipogenic or the non-induced (P < .05). Conclusions Our study demonstrates that SAK-c[RGDfC] and AK-c[RGDfC] improved adhesion and migration of PDLCs and supported osteogenic differentiation of PDLCs. These cyclic RGD peptides proved to be applicable biocompatible material in regenerative medicine. Funding 1997258 /api/funding/1997258 false true true 2020 true true true false true false GOLD 2025-12-29 true https://doi.org/10.1111/jre.12760 12 0 12 11 1 11 11 10 11 11 11 11 11 11 1 0 12 11 12 11 0 9 9 Folyóiratcikk Journal Article 24 12 Könyvrészlet Chapter in Book 25 0 Könyv Book 23 0 Egyéb konferenciaközlemény Conference paper 31 0 Egyéb konferenciakötet Conference proceedings 32 0 Oltalmi formák Protection forms 26 0 Disszertáció Thesis 28 0 Egyéb Miscellaneous 29 0 Alkotás Achievement 22 0 Kutatási adat Research data 33 0 Folyóiratcikk Journal Article 24 0 0 0 Könyvrészlet Chapter in Book 25 0 0 0 Könyv Book 23 0 0 0 Egyéb konferenciaközlemény Conference paper 31 0 0 0 Egyéb konferenciakötet Conference proceedings 32 0 0 0 Oltalmi formák Protection forms 26 0 0 0 Disszertáció Thesis 28 0 0 0 Egyéb Miscellaneous 29 0 0 0 Alkotás Achievement 22 0 0 0 Kutatási adat Research data 33 0 0 0 2021 0 2 2 2 2 2022 0 4 3 4 3 2023 0 3 3 3 3 2024 0 2 2 2 2 2025 0 1 1 1 1 Q2 false 10000509 false false false 2020-08-12T19:32:57.482+0000 Genetikai, Sejt- és Immunbiológiai Intézet (SE / AOK / I); Konzerváló Fogászati Klinika (SE / FOK); MTA-ELTE Peptidkémiai Kutatócsoport (ELTE / TTK / KI); Orálbiológiai Tanszék (SE / FOK); Orális Diagnosztikai Tanszék (SE / FOK) 9 30 5 9 0 true 10014568 Language 10002 /api/language/10002 Angol English true 2 true PersonAuthorship 95978338 /api/authorship/95978338 1 0.111 true false Author 10077582 /api/author/10077582 Khorolsuren Zambaga true true Khorolsuren Zambaga true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 91641906 /api/authorship/91641906 2 0.11111111 false false false Author 10027592 /api/author/10027592 Láng Orsolya true 10027592 true Lang Orsolya true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 91641907 /api/authorship/91641907 3 0.11111111 false false false Author 10015589 /api/author/10015589 Pállinger Éva true 10015589 true Pallinger Eva true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 91641939 /api/authorship/91641939 4 0.11111111 false false false Author 105 /api/author/105 Földes Anna true 105 true Foldes Anna true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 91641940 /api/authorship/91641940 5 0.11111111 false false false Author 10020403 /api/author/10020403 Gyulai-Gaál Szabolcs true 10020403 true Gyulai-Gaál Szabolcs true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 91641941 /api/authorship/91641941 6 0.11111111 false false false Author 10006652 /api/author/10006652 Varga Gábor true 10006652 true Varga Gabor true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 91641911 /api/authorship/91641911 7 0.11111111 false false false Author 10001614 /api/author/10001614 Mező Gábor true 10001614 true Mezo Gabor true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 91641942 /api/authorship/91641942 8 0.11111111 false false Author 10035110 /api/author/10035110 Vág János true 10035110 true Vag Janos true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 91641913 /api/authorship/91641913 9 0.11111111 false true true Author 10000509 /api/author/10000509 Kőhidai László true 10000509 true Kohidai Laszlo true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PublicationIdentifier 16915376 /api/publicationidentifier/16915376 PlainSource 6 /api/publicationsource/6 PublicationSourceType 10001 /api/publicationsourcetype/10001 true true true DOI DOI https://doi.org/@@@ true true 6 true GOLD true IDENTICAL 10.1111/jre.12760 https://doi.org/10.1111/jre.12760 false true PublicationIdentifier 17040264 /api/publicationidentifier/17040264 PlainSource 1 /api/publicationsource/1 PublicationSourceType 10003 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http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=32406091&dopt=Abstract true true Classification 12215 /api/classification/12215 true true Keyword 1501538 /api/keyword/1501538 true true Keyword 1647043 /api/keyword/1647043 true true Keyword 2011559 /api/keyword/2011559 true true Keyword 2771404 /api/keyword/2771404 true true SjrRating 10872022 /api/sjrrating/10872022 4 0.49 journal RatingType 10002 /api/ratingtype/10002 sjr true true ClassificationExternal 3506 /api/classificationexternal/3506 true 3506 true Q2 DIRECT true true /api/publication/31313575 <div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" mtid="10077582"><a href="/gui2/?type=authors&mode=browse&sel=10077582" target="_blank">Khorolsuren Zambaga (<span class="authorship-author-name">Khorolsuren Zambaga</span> <span class="authorAux-mtmt"> Medical science</span>) </a> </span> <span class="author-affil"><span title="Semmelweis University">SU</span>/<span title="Faculty of Dentistry">FD</span>/Department of Conservative Dentistry; <span title="Semmelweis University">SU</span>/<span title="Faculty of Medicine">FM</span>/<span title="Instituties">I</span>/Department of Genetics, Cell- and Immunology</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10027592"><a href="/gui2/?type=authors&mode=browse&sel=10027592" target="_blank">Lang Orsolya (<span class="authorship-author-name">Láng Orsolya</span> <span class="authorAux-mtmt"> sejtbiológia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis University">SU</span>/<span title="Faculty of Medicine">FM</span>/<span title="Instituties">I</span>/Department of Genetics, Cell- and Immunology</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10015589"><a href="/gui2/?type=authors&mode=browse&sel=10015589" target="_blank">Pallinger Eva (<span class="authorship-author-name">Pállinger Éva</span> <span class="authorAux-mtmt"> Immunológia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis University">SU</span>/<span title="Faculty of Medicine">FM</span>/<span title="Instituties">I</span>/Department of Genetics, Cell- and Immunology</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="105"><a href="/gui2/?type=authors&mode=browse&sel=105" target="_blank">Foldes Anna (<span class="authorship-author-name">Földes Anna</span> <span class="authorAux-mtmt"> Molekulár biológia, Élettan</span>) </a> </span> <span class="author-affil"><span title="Semmelweis University">SU</span>/<span title="Faculty of Dentistry">FD</span>/Department of Oral Biology</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10020403"><a href="/gui2/?type=authors&mode=browse&sel=10020403" target="_blank">Gyulai-Gaál Szabolcs (<span class="authorship-author-name">Gyulai-Gaál Szabolcs</span> <span class="authorAux-mtmt"> Szájsebészet</span>) </a> </span> <span class="author-affil"><span title="Semmelweis University">SU</span>/<span title="Faculty of Dentistry">FD</span>/Department of Oral Diagnosics</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10006652"><a href="/gui2/?type=authors&mode=browse&sel=10006652" target="_blank">Varga Gabor (<span class="authorship-author-name">Varga Gábor</span> <span class="authorAux-mtmt"> Élettan, orálbiológia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis University">SU</span>/<span title="Faculty of Dentistry">FD</span>/Department of Oral Biology</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10001614"><a href="/gui2/?type=authors&mode=browse&sel=10001614" target="_blank">Mezo Gabor (<span class="authorship-author-name">Mező Gábor</span> <span class="authorAux-mtmt"> Szerves kémia</span>) </a> </span> <span class="author-affil"><span title="Eötvös Loránd University">ELTE</span>/<span title="Faculty of Science">ELU FoS</span>/<span title="Insititute of Chemistry">IC</span>/HAS-ELTE Research Group of Peptide Chemistry</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10035110"><a href="/gui2/?type=authors&mode=browse&sel=10035110" target="_blank">Vag Janos (<span class="authorship-author-name">Vág János</span> <span class="authorAux-mtmt"> Fogászat</span>) </a> </span> <span class="author-affil"><span title="Semmelweis University">SU</span>/<span title="Faculty of Dentistry">FD</span>/Department of Conservative Dentistry</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10000509"><a href="/gui2/?type=authors&mode=browse&sel=10000509" target="_blank">Kohidai Laszlo ✉ (<span class="authorship-author-name">Kőhidai László</span> <span class="authorAux-mtmt"> Elméleti orvostudomány</span>) </a> </span> <span class="author-affil"><span title="Semmelweis University">SU</span>/<span title="Faculty of Medicine">FM</span>/<span title="Instituties">I</span>/Department of Genetics, Cell- and Immunology</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;31313575" target="_blank">Functional and cell surface characteristics of periodontal ligament cells (PDLCs) on RGD-synthetic polypeptide conjugate coatings</a></div> <div> <span class="journal-title">JOURNAL OF PERIODONTAL RESEARCH</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/0022-3484">0022-3484</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1600-0765">1600-0765</a>)</span>: <span class="journal-volume">55</span> <span class="journal-issue">5</span> <span class="page"> pp 713-723 </span> <span class="year">(2020)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Language: English | </span> <span class="identifiers"> <span class="id identifier oa_GOLD" title=" Gold "> <a style="color:blue" title="10.1111/jre.12760" target="_blank" href="https://doi.org/10.1111/jre.12760"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="000532547000001" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/000532547000001"> WoS </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="85084413701" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85084413701"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="32406091" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=32406091&dopt=Abstract"> PubMed </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Journal subject: Scopus - Periodontics&nbsp;&nbsp;&nbsp;Rank:&nbsp;Q2</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="" class="citingPub-count">Citing papers: 12</span> | Independent citation: 11 | Self citation: 1 | Unknown citation: 0 | Number of citations in WoS: 10 | Number of citations in Scopus:&nbsp;11 | WoS/Scopus assigned:&nbsp;11 | Number of citations with DOI:&nbsp;11 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;31313575&sort=publishedYear,desc&sort=title"> Number of cited publications: 9 </a> </div> <div class="mtid"><span class="long-pub-mtid">Publication: 31313575</span> | <span class="status-data status-VALIDATED"> Validated </span> Core Citing | <span class="type-subtype">Journal Article ( Article ) </span> | <span class="pub-category">Scientific</span> | <span class="publication-sourceOfData">RIS-Egyéb</span> </div> <div class="funder"> EMBERI ERŐFORRÁS FEJLESZTÉSI OPERATÍV PROGRAM (EFOP)(EFOP-3.6.2-162017-00006) Funder: NRDIO </div> <div class="lastModified">Last Modified: 2021.09.21. 10:09 Tamásné Gere (SE_KK_Admin5_GT, admin) </div> <div class="lockedBy">Centrally managed 2023.08.22. 15:58 Andrea Vándor (VA, admin) </div> <pre class="comment" style="margin-top: 0; margin-bottom: 0;"><u>Comments</u>: Funding Agency and Grant Number: Hungarian Scientific Research FundOrszagos Tudomanyos Kutatasi Alapprogramok (OTKA) [K112364]; Stipendium Hungaricum Tempus Public Foundation Funding text: Hungarian Scientific Research Fund, Grant/Award Number: K112364; Stipendium Hungaricum Tempus Public Foundation</pre> </div></div>