(NVKP-16-1-2016-0017 National Heart Program) Támogató: NKFIH
(ÚNKP-19-4)
(UNKP-19-3-I-SE-60)
Szakterületek:
Általános orvostudomány
Farmakológia és gyógyszerészet
Szív és érrendszer
Swiprosin-1 (EFhD2) is a molecule that triggers structural adaptation of isolated
adult rat cardiomyocytes to cell culture conditions by initiating a process known
as cell spreading. This process mimics central aspects of cardiac remodeling, as it
occurs subsequent to myocardial infarction. However, expression of swiprosin-1 in
cardiac tissue and its regulation in vivo has not yet been addressed. The expression
of swiprosin-1 was analyzed in mice, rat, and pig hearts undergoing myocardial infarction
or ischemia/reperfusion with or without cardiac protection by ischemic pre- and postconditioning.
In mouse hearts, swiprosin-1 protein expression was increased after 4 and 7 days in
myocardial infarct areas specifically in cardiomyocytes as verified by immunoblotting
and histology. In rat hearts, swiprosin-1 mRNA expression was induced within 7 days
after ischemia/reperfusion but this induction was abrogated by conditioning. As in
cultured cardiomyocytes, the expression of swiprosin-1 was associated with a coinduction
of arrestin-2, suggesting a common mechanism of regulation. Rno-miR-32-3p and rno-miR-34c-3p
were associated with the regulation pattern of both molecules. Moreover, induction
of swiprosin-1 and ssc-miR-34c was also confirmed in the infarct zone of pigs. In
summary, our data show that up-regulation of swiprosin-1 appears in the postischemic
heart during cardiac remodeling and repair in different species.
