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Center for Developmental Biology, College of Life Science, Anhui Agricultural University, No. 130, Changjiang Road, Hefei, Anhui 230036, China
Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China
Cited By :5
Export Date: 29 April 2020
Correspondence Address: Wang, Z.; Center for Developmental Biology, College of Life Science, Anhui Agricultural University, No. 130, Changjiang Road, China; email: wangzaigui2013@163.com
Funding details: AHCYTX-10
Funding text 1: Data accessibility. The data supporting this article are available on Dryad: (https://doi.org/10.5061/dryad.27j11) [62]. Authors’ contributions. Z.W. designed, performed and wrote the experiments, and helped draft the manuscript. M.Y .and L.S. carried out the laboratory work, participated in data analysis, participated in the design of the study and drafted the manuscript. R.Y. collected field data. All authors gave final approval for publication. K.Q. revised the manuscript. Competing interests. We declare we have no competing interests. Funding. This work was supported by the Major Science and Technology Program of Anhui Province (2017), Anhui Industry and Technology System of Poultry Science (AHCYTX-10), Management and Demonstration of Quality
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The optimization of fermentation conditions for producing cellulase of bacillus amyloliquefaciens and its application to goose feed
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The proper culture conditions for producing cellulase of Bacillus amyloliquefaciens S1, isolated from the cecum of goose was optimized by single-factor experiment combined with orthogonal test. The properties of the cellulase were investigated by DNS method. The appropriate doses of B. amyloliquefaciens S1 were obtained by adding them to goose feed. It indicated that the suitable culture conditions of producing cellulase were the culture temperature of 37°C, the initial pH of 7.0, the incubation time of 72 h and the loaded liquid volume of 75 ml per 250 ml. The effects of each factor on producing cellulase by B. amyloliquefaciens S1 were as follows: initial pH > incubation time = culture temperature > loaded liquid volume. The optimum reaction temperature and pH were 50°C and 7.0, respectively. This enzyme is a kind of neutral cellulase that possesses resistance to heat and acidity. It showed high activity to absorbent cotton, soya bean meal and filter paper. By adding different doses of B. amyloliquefaciens S1 to the goose feed, it was found that the egg production, average egg weight, fertilization rate and the hatching rate were promoted both in experiment 1 (1.5 g kg−1) and experiment 2 (3 g kg−1). Also the difference of egg production, fertilization rate and hatching rate between experiment 1 and control group was obvious (p < 0.05), and the average egg weight was significantly increased in experiment 2 (p < 0.05). © 2017 The Authors.
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<div class="JournalArticle Publication short-list"> <div class="authors"> <span class="author-name" > Ye, M. </span> <span class="author-type"> </span> ; <span class="author-name" > Sun, L. </span> <span class="author-type"> </span> ; <span class="author-name" > Yang, R. </span> <span class="author-type"> </span> ; <span class="author-name" > Wang, Z. ✉ </span> <span class="author-type"> </span> ; <span class="author-name" > Qi, K. </span> <span class="author-type"> </span> </div ><div class="title"><a href="/gui2/?mode=browse¶ms=publication;31294976" mtid="31294976" target="_blank">The optimization of fermentation conditions for producing cellulase of bacillus amyloliquefaciens and its application to goose feed</a></div> <div class="pub-info"> <span class="journal-title">ROYAL SOCIETY OPEN SCIENCE</span> <span class="journal-volume">4</span> : <span class="journal-issue">10</span> <span class="page"> Paper: 171012 </span> <span class="year">(2017)</span> </div> <div class="pub-end"><div class="identifier-list"> <span class="identifiers"> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="10.1098/rsos.171012" target="_blank" href="https://doi.org/10.1098%2Frsos.171012"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="000413965600042" target="_blank" href="http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=CustomerName&SrcApp=CustomerName&DestLinkType=FullRecord&KeyUT=000413965600042&DestApp=WOS"> WoS </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="85032350521" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85032350521"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:black" title="http://rsos.royalsocietypublishing.org/content/royopensci/4/10/171012.full.pdf" target="_blank" href="http://rsos.royalsocietypublishing.org/content/royopensci/4/10/171012.full.pdf"> Teljes dokumentum </a> </span> </span> </div> <span class="short-pub-prop-list"> <span class="short-pub-mtid"> Közlemény:31294976 </span> <span class="status-holder"><span class="status-data status-APPROVED"> Nyilvános </span></span> <span class="pub-core"> Idéző</span> <span class="pub-type">Folyóiratcikk (Szakcikk ) </span> <!-- && !record.category.scientific --> <span class="pub-category">Tudományos</span> </span> </div> </div><div class="JournalArticle Publication long-list">
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<div class="autype autype0"> <span class="author-name" >Ye M.
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;
<span class="author-name" >Sun L.
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<span class="author-name" >Yang R.
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;
<span class="author-name" >Wang Z. ✉
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<div class="title"><a href="/gui2/?mode=browse¶ms=publication;31294976" target="_blank">The optimization of fermentation conditions for producing cellulase of bacillus amyloliquefaciens and its application to goose feed</a></div> <div> <span class="journal-title">ROYAL SOCIETY OPEN SCIENCE</span>
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<div class="lastModified">Utolsó módosítás: 2021.12.15. 16:24 Bikádi Katalin (Állatorvostud. Egy. 4, admin)
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<pre class="comment" style="margin-top: 0; margin-bottom: 0;"><u>Megjegyzés</u>: Center for Developmental Biology, College of Life Science, Anhui Agricultural University, No. 130, Changjiang Road, Hefei, Anhui 230036, China
Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China
Cited By :5
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