https://m2.mtmt.hu/ eng 2026-10-23 22:01 JournalArticle 31240593 VALIDATED true Research Centre for Natural Sciences, Institute of Enzymology, Budapest, Hungary South-Pest Hospital Centre, National Institute of Hematology and Infectious Diseases, Laboratory of Molecular and Cytogenetics, Budapest, Hungary Cited By :9 Export Date: 20 October 2022 CODEN: BCPCA Correspondence Address: Sarkadi, B.; Research Centre for Natural Sciences, Rm. D2.03A, Magyar tudósok körútja 2., Hungary; email: sarkadi.balazs@ttk.hu Chemicals/CAS: cisplatin, 15663-27-1, 26035-31-4, 96081-74-2; clemastine, 15686-51-8; cobalt chloride, 1332-82-7, 7646-79-9; colchicine, 64-86-8; dexamethasone, 50-02-2; docetaxel, 114977-28-5; etoposide, 33419-42-0, 433304-61-1; flavopiridol, 131740-09-5, 146426-40-6; gemcitabine, 103882-84-4; hydrocortisone, 50-23-7; methylprednisolone, 6923-42-8, 83-43-2; mitoxantrone, 65271-80-9, 70476-82-3; paclitaxel, 33069-62-4; sodium ion, 17341-25-2; valproic acid, 1069-66-5, 99-66-1; vinblastine, 865-21-4; vorinostat, 149647-78-9; epidermal growth factor receptor, 79079-06-4; ABCG2 protein, human; Antineoplastic Agents; ATP Binding Cassette Transporter, Subfamily G, Member 2; CRISPR-Associated Protein 9; EGFR protein, human; ErbB Receptors; Neoplasm Proteins 0 false 2026-10-20T07:51:01.878+0000 2022-02-17T10:41:01.403+0000 2020-03-13T10:29:04.657+0000 true 1241938 Sarkadi Balázs /api/author/1241938 Author true 1241938 2026-08-28T06:12:31.585+0000 2020-04-29T10:26:12.040+0000 true 10026589 Tichy Rita /api/admin/10026589 Admin true 10026589 Admin 10073918 /api/admin/10073918 Kovács Marcell true true 2024-03-22T13:59:27.989+0000 true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Kovacsics, Daniella Precision-engineered reporter cell lines reveal ABCG2 regulation in live lung cancer cells true true /api/journal/686 REVIEWED true false 686 false 686 true 0006-2952 1873-2968 Journal FOREIGN 175 113865 113865 14 2020 Expression of the ABCG2 multidrug transporter is a marker of cancer stem cells and a predictor of recurrent malignant disease. Understanding how human ABCG2 expression is modulated by pharmacotherapy is crucial in guiding therapeutic recommendations and may aid rational drug development. Genome edited reporter cells are useful in investigating gene regulation and visualizing protein activity in live cells but require precise targeting to preserve native regulatory regions. Here, we describe a fluorescent reporter assay that allows the noninvasive assessment of ABCG2 regulation in human lung adenocarcinoma cells. Using CRISPR-Cas9 gene editing coupled with homology-directed repair, we targeted an eGFP coding sequence to the translational start site of ABCG2, generating ABCG2 knock-out and in situ tagged ABCG2 reporter cells. Using the engineered cell lines, we show that ABCG2 is upregulated by a number of anti-cancer medications, HDAC inhibitors, hypoxia-mimicking agents and glucocorticoids, supporting a model in which ABCG2 is under the control of a general stress response. To our knowledge, this is the first description of a fluorescent reporter assay system designed to follow the endogenous regulation of a human ABC transporter in live cells. The information gained may guide therapy recommendations and aid rational drug design. 2020 true true true false true false NONE 2026-10-20 false 19 0 19 16 4 19 19 17 19 19 19 16 16 16 3 0 19 16 19 16 0 15 15 Folyóiratcikk Journal Article 24 18 Könyvrészlet Chapter in Book 25 1 Könyv Book 23 0 Egyéb konferenciaközlemény Conference paper 31 0 Egyéb konferenciakötet Conference proceedings 32 0 Oltalmi formák Protection forms 26 0 Disszertáció Thesis 28 0 Egyéb Miscellaneous 29 0 Alkotás Achievement 22 0 Kutatási adat Research data 33 0 Folyóiratcikk Journal Article 24 0 0 0 Könyvrészlet Chapter in Book 25 0 0 0 Könyv Book 23 0 0 0 Egyéb konferenciaközlemény Conference paper 31 0 0 0 Egyéb konferenciakötet Conference proceedings 32 0 0 0 Oltalmi formák Protection forms 26 0 0 0 Disszertáció Thesis 28 0 0 0 Egyéb Miscellaneous 29 0 0 0 Alkotás Achievement 22 0 0 0 Kutatási adat Research data 33 0 0 0 2020 0 1 0 1 0 2021 0 8 7 8 7 2022 0 0 0 0 0 2023 0 4 4 4 4 2024 0 2 2 2 2 2025 0 1 1 1 1 2026 0 3 2 3 2 Q1 false 10067874,1241938,10040526 false false false 2020-04-28T10:26:11.580+0000 Biomembrán Kutatócsoport (TTK / EI); Enzimológiai Intézet (TTK) 13 67 2 15 0 true 10073918 Language 10002 /api/language/10002 Angol English true 2 true PersonAuthorship 91125769 /api/authorship/91125769 1 0.06666667 true false false Author 10058367 /api/author/10058367 Kovacsics Daniella true 10058367 true Kovacsics Daniella true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 91125770 /api/authorship/91125770 2 0.06666667 true false false Author 10027530 /api/author/10027530 Brózik Anna true 10027530 true Brózik Anna true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 91125771 /api/authorship/91125771 3 0.06666667 false false false Author 10034873 /api/author/10034873 Tihanyi Borbála true 10034873 true Tihanyi Borbála true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 91125772 /api/authorship/91125772 4 0.06666667 false false false Author 10043362 /api/author/10043362 Matula Zsolt true 10043362 true Matula Zsolt true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 91125773 /api/authorship/91125773 5 0.06666667 false false false Borsy Adrienn true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 91125774 /api/authorship/91125774 6 0.06666667 false false false Mészáros Nikolett true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 91125775 /api/authorship/91125775 7 0.06666667 false false false Author 10040526 /api/author/10040526 Szabó Edit Zsuzsanna true 10040526 true Szabó Edit true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 91125776 /api/authorship/91125776 8 0.06666667 false false false Author 10040185 /api/author/10040185 Németh Eszter true 10040185 true Németh Eszter true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 91125777 /api/authorship/91125777 9 0.06666667 false false false Author 10067874 /api/author/10067874 Fóthi Ábel true true Fóthi Ábel true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 91125778 /api/authorship/91125778 10 0.06666667 false false false Author 10053932 /api/author/10053932 Zámbó Boglárka true 10053932 true Zámbó Boglárka true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 91125779 /api/authorship/91125779 11 0.06666667 false false false Author 10026660 /api/author/10026660 Szüts Dávid true 10026660 true Szüts Dávid true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 91125780 /api/authorship/91125780 12 0.06666667 false false false Author 10015754 /api/author/10015754 Várady György true 10015754 true Várady György true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 91125781 /api/authorship/91125781 13 0.06666667 false false false Author 10000148 /api/author/10000148 Orbán Tamás I. true 10000148 true Orbán Tamás I true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 91125782 /api/authorship/91125782 14 0.06666667 false false false Author 10027529 /api/author/10027529 Apáti Ágota true 10027529 true Apáti Ágota true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 91125783 /api/authorship/91125783 15 0.06666667 false true true Author 1241938 /api/author/1241938 Sarkadi Balázs true 1241938 true Sarkadi Balázs true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PublicationIdentifier 16755398 /api/publicationidentifier/16755398 PlainSource 6 /api/publicationsource/6 PublicationSourceType 10001 /api/publicationsourcetype/10001 true true true DOI DOI https://doi.org/@@@ true true 6 true IDENTICAL 10.1016/j.bcp.2020.113865 https://doi.org/10.1016/j.bcp.2020.113865 false true PublicationIdentifier 16914075 /api/publicationidentifier/16914075 PlainSource 1 /api/publicationsource/1 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true WoS WoS https://www.webofscience.com/wos/woscc/full-record/@@@ true true 1 true IDENTICAL 000527350300011 https://www.webofscience.com/wos/woscc/full-record/000527350300011 false true PublicationIdentifier 16800379 /api/publicationidentifier/16800379 PlainSource 3 /api/publicationsource/3 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true Scopus Scopus http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-@@@ true true 3 true IDENTICAL 85081239314 http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85081239314 false true PublicationIdentifier 16755397 /api/publicationidentifier/16755397 PlainSource 17 /api/publicationsource/17 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true PubMed PubMed http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=@@@&dopt=Abstract true true 17 true IDENTICAL 32142727 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=32142727&dopt=Abstract false true PublicationIdentifier 31583495 /api/publicationidentifier/31583495 PlainSource 40 /api/publicationsource/40 PublicationSourceType 10006 /api/publicationsourcetype/10006 true true true Egyéb URL Other URL @@@ true true 40 true NONE IDENTICAL https://www.scopus.com/inward/record.uri?eid=2-s2.0-85081239314&doi=10.1016%2Fj.bcp.2020.113865&partnerID=40&md5=afefff71d23d9f62a822daeacb925b74 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85081239314&doi=10.1016%2Fj.bcp.2020.113865&partnerID=40&md5=afefff71d23d9f62a822daeacb925b74 false true Classification 10575 /api/classification/10575 true true Keyword 8268 /api/keyword/8268 true 8268 true Keyword 19546 /api/keyword/19546 true 19546 true Keyword 1431489 /api/keyword/1431489 true 1431489 true Keyword 1815917 /api/keyword/1815917 true true Keyword 1995227 /api/keyword/1995227 true true SjrRating 10750627 /api/sjrrating/10750627 82 0.24 journal RatingType 10002 /api/ratingtype/10002 sjr true true ClassificationExternal 1303 /api/classificationexternal/1303 true 1303 true Q1 DIRECT true true /api/publication/31240593 <div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" mtid="10058367"><a href="/gui2/?type=authors&mode=browse&sel=10058367" target="_blank">Kovacsics Daniella (<span class="authorship-author-name">Kovacsics Daniella</span> <span class="authorAux-mtmt"> Biológia</span>) </a> </span> <span class="author-affil"><span title="Research Centre for Natural Sciences">RCNS</span>/Institute of Enzymology</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10027530"><a href="/gui2/?type=authors&mode=browse&sel=10027530" target="_blank">Brózik Anna<sup>*</sup> (<span class="authorship-author-name">Brózik Anna</span> <span class="authorAux-mtmt"> Biológia</span>) </a> </span> <span class="author-affil"><span title="Research Centre for Natural Sciences">RCNS</span>/Institute of Enzymology</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10034873"><a href="/gui2/?type=authors&mode=browse&sel=10034873" target="_blank">Tihanyi Borbála (<span class="authorship-author-name">Tihanyi Borbála</span> <span class="authorAux-mtmt"> Molekuláris biológia</span>) </a> </span> <span class="author-affil"><span title="Research Centre for Natural Sciences">RCNS</span>/Institute of Enzymology</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10043362"><a href="/gui2/?type=authors&mode=browse&sel=10043362" target="_blank">Matula Zsolt (<span class="authorship-author-name">Matula Zsolt</span> <span class="authorAux-mtmt"> Őssejt-biológia, Genetika</span>) </a> </span> <span class="author-affil"><span title="Research Centre for Natural Sciences">RCNS</span>/Institute of Enzymology</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Borsy Adrienn </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Mészáros Nikolett </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10040526"><a href="/gui2/?type=authors&mode=browse&sel=10040526" target="_blank">Szabó Edit (<span class="authorship-author-name">Szabó Edit Zsuzsanna</span> <span class="authorAux-mtmt"> biológus</span>) </a> </span> <span class="author-affil"><span title="Research Centre for Natural Sciences">RCNS</span>/Institute of Enzymology</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10040185"><a href="/gui2/?type=authors&mode=browse&sel=10040185" target="_blank">Németh Eszter (<span class="authorship-author-name">Németh Eszter</span> <span class="authorAux-mtmt"> Bioinformatika</span>) </a> </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10067874"><a href="/gui2/?type=authors&mode=browse&sel=10067874" target="_blank">Fóthi Ábel (<span class="authorship-author-name">Fóthi Ábel</span> <span class="authorAux-mtmt"> genetika</span>) </a> </span> <span class="author-affil"><span title="Research Centre for Natural Sciences">RCNS</span>/<span title="Institute of Enzymology">EI</span>/Enzim_413</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10053932"><a href="/gui2/?type=authors&mode=browse&sel=10053932" target="_blank">Zámbó Boglárka (<span class="authorship-author-name">Zámbó Boglárka</span> <span class="authorAux-mtmt"> molekuláris biológia</span>) </a> </span> <span class="author-affil"><span title="Research Centre for Natural Sciences">RCNS</span>/Institute of Enzymology</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10026660"><a href="/gui2/?type=authors&mode=browse&sel=10026660" target="_blank">Szüts Dávid (<span class="authorship-author-name">Szüts Dávid</span> <span class="authorAux-mtmt"> Molekuláris biológia</span>) </a> </span> <span class="author-affil"><span title="Research Centre for Natural Sciences">RCNS</span>/Institute of Enzymology</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10015754"><a href="/gui2/?type=authors&mode=browse&sel=10015754" target="_blank">Várady György (<span class="authorship-author-name">Várady György</span> <span class="authorAux-mtmt"> Membránbiológia</span>) </a> </span> <span class="author-affil"><span title="Research Centre for Natural Sciences">RCNS</span>/Institute of Enzymology</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10000148"><a href="/gui2/?type=authors&mode=browse&sel=10000148" target="_blank">Orbán Tamás I (<span class="authorship-author-name">Orbán Tamás I.</span> <span class="authorAux-mtmt"> Molekuláris sejtbiológia</span>) </a> </span> <span class="author-affil"><span title="Research Centre for Natural Sciences">RCNS</span>/Institute of Enzymology</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10027529"><a href="/gui2/?type=authors&mode=browse&sel=10027529" target="_blank">Apáti Ágota (<span class="authorship-author-name">Apáti Ágota</span> <span class="authorAux-mtmt"> Őssejt kutatás</span>) </a> </span> <span class="author-affil"><span title="Research Centre for Natural Sciences">RCNS</span>/Institute of Enzymology</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="1241938"><a href="/gui2/?type=authors&mode=browse&sel=1241938" target="_blank">Sarkadi Balázs ✉ (<span class="authorship-author-name">Sarkadi Balázs</span> <span class="authorAux-mtmt"> biofizika</span>) </a> </span> <span class="author-affil"><span title="Research Centre for Natural Sciences">RCNS</span>/Institute of Enzymology</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;31240593" target="_blank">Precision-engineered reporter cell lines reveal ABCG2 regulation in live lung cancer cells</a></div> <div> <span class="journal-title">BIOCHEMICAL PHARMACOLOGY</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/0006-2952">0006-2952</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1873-2968">1873-2968</a>)</span>: <span class="journal-volume">175</span> <span class="page"> Paper 113865. 14 p. </span> <span class="year">(2020)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Language: English | </span> <span class="identifiers"> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="10.1016/j.bcp.2020.113865" target="_blank" href="https://doi.org/10.1016/j.bcp.2020.113865"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="000527350300011" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/000527350300011"> WoS </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="85081239314" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85081239314"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="32142727" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=32142727&dopt=Abstract"> PubMed </a> </span> <span class="id identifier oa_NONE" title=" None "> <a style="color:blue" title="https://www.scopus.com/inward/record.uri?eid=2-s2.0-85081239314&doi=10.1016%2Fj.bcp.2020.113865&partnerID=40&md5=afefff71d23d9f62a822daeacb925b74" target="_blank" href="https://www.scopus.com/inward/record.uri?eid=2-s2.0-85081239314&doi=10.1016%2Fj.bcp.2020.113865&partnerID=40&md5=afefff71d23d9f62a822daeacb925b74"> Other URL </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Journal subject: Scopus - Biochemistry&nbsp;&nbsp;&nbsp;Rank:&nbsp;Q1</div> <div class="journal-subject">Journal subject: Scopus - Pharmacology&nbsp;&nbsp;&nbsp;Rank:&nbsp;Q1</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="" class="citingPub-count">Citing papers: 19</span> | Independent citation: 16 | Self citation: 3 | Unknown citation: 0 | Number of citations in WoS: 17 | Number of citations in Scopus:&nbsp;19 | WoS/Scopus assigned:&nbsp;19 | Number of citations with DOI:&nbsp;19 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;31240593&sort=publishedYear,desc&sort=title"> Number of cited publications: 15 </a> </div> <div class="mtid"><span class="long-pub-mtid">Publication: 31240593</span> | <span class="status-data status-VALIDATED"> Validated </span> Core Citing | <span class="type-subtype">Journal Article ( Article ) </span> | <span class="pub-category">Scientific</span> | <span class="publication-sourceOfData">PubMed XML</span> </div> <div class="lastModified">Last Modified: 2022.02.17. 11:41 Zsolt Matula (Őssejt-biológia, Genetika) </div> <div class="lockedBy">Centrally managed 2024.03.22. 14:59 Marcell Kovács (MTMT Közp., admin) </div> <pre class="comment" style="margin-top: 0; margin-bottom: 0;"><u>Comments</u>: Research Centre for Natural Sciences, Institute of Enzymology, Budapest, Hungary South-Pest Hospital Centre, National Institute of Hematology and Infectious Diseases, Laboratory of Molecular and Cytogenetics, Budapest, Hungary Cited By :9 Export Date: 20 October 2022 CODEN: BCPCA ...</pre> </div></div>