https://m2.mtmt.hu/ hun 2026-09-22 06:43 JournalArticle 31240593 VALIDATED true Research Centre for Natural Sciences, Institute of Enzymology, Budapest, Hungary South-Pest Hospital Centre, National Institute of Hematology and Infectious Diseases, Laboratory of Molecular and Cytogenetics, Budapest, Hungary Cited By :9 Export Date: 20 October 2022 CODEN: BCPCA Correspondence Address: Sarkadi, B.; Research Centre for Natural Sciences, Rm. D2.03A, Magyar tudósok körútja 2., Hungary; email: sarkadi.balazs@ttk.hu Chemicals/CAS: cisplatin, 15663-27-1, 26035-31-4, 96081-74-2; clemastine, 15686-51-8; cobalt chloride, 1332-82-7, 7646-79-9; colchicine, 64-86-8; dexamethasone, 50-02-2; docetaxel, 114977-28-5; etoposide, 33419-42-0, 433304-61-1; flavopiridol, 131740-09-5, 146426-40-6; gemcitabine, 103882-84-4; hydrocortisone, 50-23-7; methylprednisolone, 6923-42-8, 83-43-2; mitoxantrone, 65271-80-9, 70476-82-3; paclitaxel, 33069-62-4; sodium ion, 17341-25-2; valproic acid, 1069-66-5, 99-66-1; vinblastine, 865-21-4; vorinostat, 149647-78-9; epidermal growth factor receptor, 79079-06-4; ABCG2 protein, human; Antineoplastic Agents; ATP Binding Cassette Transporter, Subfamily G, Member 2; CRISPR-Associated Protein 9; EGFR protein, human; ErbB Receptors; Neoplasm Proteins 0 false 2026-08-28T06:13:36.354+0000 2022-02-17T10:41:01.403+0000 2020-03-13T10:29:04.657+0000 true 1241938 Sarkadi Balázs /api/author/1241938 Author true 1241938 2026-08-28T06:12:31.585+0000 2020-04-29T10:26:12.040+0000 true 10026589 Tichy Rita /api/admin/10026589 Admin true 10026589 Admin 10073918 /api/admin/10073918 Kovács Marcell true true 2024-03-22T13:59:27.989+0000 true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Kovacsics, Daniella Precision-engineered reporter cell lines reveal ABCG2 regulation in live lung cancer cells true true /api/journal/686 REVIEWED true false 686 false 686 true 0006-2952 1873-2968 Journal FOREIGN 175 113865 113865 14 2020 Expression of the ABCG2 multidrug transporter is a marker of cancer stem cells and a predictor of recurrent malignant disease. Understanding how human ABCG2 expression is modulated by pharmacotherapy is crucial in guiding therapeutic recommendations and may aid rational drug development. Genome edited reporter cells are useful in investigating gene regulation and visualizing protein activity in live cells but require precise targeting to preserve native regulatory regions. Here, we describe a fluorescent reporter assay that allows the noninvasive assessment of ABCG2 regulation in human lung adenocarcinoma cells. Using CRISPR-Cas9 gene editing coupled with homology-directed repair, we targeted an eGFP coding sequence to the translational start site of ABCG2, generating ABCG2 knock-out and in situ tagged ABCG2 reporter cells. Using the engineered cell lines, we show that ABCG2 is upregulated by a number of anti-cancer medications, HDAC inhibitors, hypoxia-mimicking agents and glucocorticoids, supporting a model in which ABCG2 is under the control of a general stress response. To our knowledge, this is the first description of a fluorescent reporter assay system designed to follow the endogenous regulation of a human ABC transporter in live cells. 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/api/keyword/19546 true 19546 true Keyword 1431489 /api/keyword/1431489 true 1431489 true Keyword 1815917 /api/keyword/1815917 true true Keyword 1995227 /api/keyword/1995227 true true SjrRating 10750627 /api/sjrrating/10750627 82 0.24 journal RatingType 10002 /api/ratingtype/10002 sjr true true ClassificationExternal 1303 /api/classificationexternal/1303 true 1303 true Q1 DIRECT true true /api/publication/31240593 <div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" mtid="10058367"><a href="/gui2/?type=authors&mode=browse&sel=10058367" target="_blank">Kovacsics Daniella (<span class="authorship-author-name">Kovacsics Daniella</span> <span class="authorAux-mtmt"> Biológia</span>) </a> </span> <span class="author-affil"><span title="Természettudományi Kutatóközpont">TTK</span>/Enzimológiai Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10027530"><a href="/gui2/?type=authors&mode=browse&sel=10027530" target="_blank">Brózik Anna<sup>*</sup> (<span class="authorship-author-name">Brózik Anna</span> <span class="authorAux-mtmt"> Biológia</span>) </a> </span> <span class="author-affil"><span title="Természettudományi Kutatóközpont">TTK</span>/Enzimológiai Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10034873"><a href="/gui2/?type=authors&mode=browse&sel=10034873" target="_blank">Tihanyi Borbála (<span class="authorship-author-name">Tihanyi Borbála</span> <span class="authorAux-mtmt"> Molekuláris biológia</span>) </a> </span> <span class="author-affil"><span title="Természettudományi Kutatóközpont">TTK</span>/Enzimológiai Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10043362"><a href="/gui2/?type=authors&mode=browse&sel=10043362" target="_blank">Matula Zsolt (<span class="authorship-author-name">Matula Zsolt</span> <span class="authorAux-mtmt"> Őssejt-biológia, Genetika</span>) </a> </span> <span class="author-affil"><span title="Természettudományi Kutatóközpont">TTK</span>/Enzimológiai Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Borsy Adrienn </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Mészáros Nikolett </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10040526"><a href="/gui2/?type=authors&mode=browse&sel=10040526" target="_blank">Szabó Edit (<span class="authorship-author-name">Szabó Edit Zsuzsanna</span> <span class="authorAux-mtmt"> biológus</span>) </a> </span> <span class="author-affil"><span title="Természettudományi Kutatóközpont">TTK</span>/Enzimológiai Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10040185"><a href="/gui2/?type=authors&mode=browse&sel=10040185" target="_blank">Németh Eszter (<span class="authorship-author-name">Németh Eszter</span> <span class="authorAux-mtmt"> Bioinformatika</span>) </a> </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10067874"><a href="/gui2/?type=authors&mode=browse&sel=10067874" target="_blank">Fóthi Ábel (<span class="authorship-author-name">Fóthi Ábel</span> <span class="authorAux-mtmt"> genetika</span>) </a> </span> <span class="author-affil"><span title="Természettudományi Kutatóközpont">TTK</span>/<span title="Enzimológiai Intézet">EI</span>/Biomembrán Kutatócsoport</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10053932"><a href="/gui2/?type=authors&mode=browse&sel=10053932" target="_blank">Zámbó Boglárka (<span class="authorship-author-name">Zámbó Boglárka</span> <span class="authorAux-mtmt"> molekuláris biológia</span>) </a> </span> <span class="author-affil"><span title="Természettudományi Kutatóközpont">TTK</span>/Enzimológiai Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10026660"><a href="/gui2/?type=authors&mode=browse&sel=10026660" target="_blank">Szüts Dávid (<span class="authorship-author-name">Szüts Dávid</span> <span class="authorAux-mtmt"> Molekuláris biológia</span>) </a> </span> <span class="author-affil"><span title="Természettudományi Kutatóközpont">TTK</span>/Enzimológiai Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10015754"><a href="/gui2/?type=authors&mode=browse&sel=10015754" target="_blank">Várady György (<span class="authorship-author-name">Várady György</span> <span class="authorAux-mtmt"> Membránbiológia</span>) </a> </span> <span class="author-affil"><span title="Természettudományi Kutatóközpont">TTK</span>/Enzimológiai Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10000148"><a href="/gui2/?type=authors&mode=browse&sel=10000148" target="_blank">Orbán Tamás I (<span class="authorship-author-name">Orbán Tamás I.</span> <span class="authorAux-mtmt"> Molekuláris sejtbiológia</span>) </a> </span> <span class="author-affil"><span title="Természettudományi Kutatóközpont">TTK</span>/Enzimológiai Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10027529"><a href="/gui2/?type=authors&mode=browse&sel=10027529" target="_blank">Apáti Ágota (<span class="authorship-author-name">Apáti Ágota</span> <span class="authorAux-mtmt"> Őssejt kutatás</span>) </a> </span> <span class="author-affil"><span title="Természettudományi Kutatóközpont">TTK</span>/Enzimológiai Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="1241938"><a href="/gui2/?type=authors&mode=browse&sel=1241938" target="_blank">Sarkadi Balázs ✉ (<span class="authorship-author-name">Sarkadi Balázs</span> <span class="authorAux-mtmt"> biofizika</span>) </a> </span> <span class="author-affil"><span title="Természettudományi Kutatóközpont">TTK</span>/Enzimológiai Intézet</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;31240593" target="_blank">Precision-engineered reporter cell lines reveal ABCG2 regulation in live lung cancer cells</a></div> <div> <span class="journal-title">BIOCHEMICAL PHARMACOLOGY</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/0006-2952">0006-2952</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1873-2968">1873-2968</a>)</span>: <span class="journal-volume">175</span> <span class="page"> Paper 113865. 14 p. </span> <span class="year">(2020)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Nyelv: Angol | </span> <span class="identifiers"> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="10.1016/j.bcp.2020.113865" target="_blank" href="https://doi.org/10.1016/j.bcp.2020.113865"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="000527350300011" target="_blank" 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class="ratings"> <div class="journal-subject">Folyóirat szakterülete: Scopus - Biochemistry&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Pharmacology&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q1</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="Nyilvános idézőközlemények összesen, említések nélkül" class="citingPub-count">Nyilvános idéző összesen: 17</span> | Független: 15 | Függő: 2 | Nem jelölt: 0 | WoS jelölt: 15 | Scopus jelölt:&nbsp;17 | WoS/Scopus jelölt:&nbsp;17 | DOI jelölt:&nbsp;17 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;31240593&sort=publishedYear,desc&sort=title"> Idézett közlemények száma: 15 </a> </div> <div class="mtid"><span class="long-pub-mtid">Közlemény: 31240593</span> | <span class="status-data status-VALIDATED"> Egyeztetett </span> Forrás Idéző | <span class="type-subtype">Folyóiratcikk ( Szakcikk ) </span> | <span class="pub-category">Tudományos</span> | <span class="publication-sourceOfData">PubMed XML</span> </div> <div class="lastModified">Utolsó módosítás: 2022.02.17. 11:41 Matula Zsolt (Őssejt-biológia, Genetika) </div> <div class="lockedBy">Központi kezelésű 2024.03.22. 14:59 Kovács Marcell (MTMT Közp., admin) </div> <pre class="comment" style="margin-top: 0; margin-bottom: 0;"><u>Megjegyzés</u>: Research Centre for Natural Sciences, Institute of Enzymology, Budapest, Hungary South-Pest Hospital Centre, National Institute of Hematology and Infectious Diseases, Laboratory of Molecular and Cytogenetics, Budapest, Hungary Cited By :9 Export Date: 20 October 2022 CODEN: BCPCA ...</pre> </div></div>