A model is presented for enhancement of fluid flow through bone matrix and any porous
tissue engineering scaffold implanted within it. The mechanism of enhancement is the
skeletal muscle pump in compartments adjacent to the bone. Pressure waves from muscle
pump contractions aided by increased blood pressure during exercise coupled with temporary
occlusion of arteries leading to and veins from the bone, increase hydraulic pressure
in cortical bone capillaries so as to amplify capillary filtration. It is proposed
that capillary filtration increase is sufficiently convective to contribute to bone
fluid flow and associated percolation through tissue engineered scaffold matrix implants.
Importance of this contribution is its relative role in maintaining seeded cells in
bioreactor scaffolds. Validation of the hypothesis starts at a minimum level of demonstrating
that capillary filtration is convective. At a maximum level confirmation of the hypothesis
requires demonstration that capillary filtration-based interstitial flow is sufficient
to stimulate not only host bone cells (as proposed in part I of the hypothesis) but
bioreactor-seeded cells as well. Preliminary data is presented supporting the prediction
that skeletal muscle contraction generates convective capillary filtration.