https://m2.mtmt.hu/ eng 2026-10-23 04:50 JournalArticle 31203798 ADMIN_APPROVED true Institute of Enzymology, Research Centre of Natural Sciences of the Hungarian Academy of Sciences, Budapest, H-1117, Hungary Doctoral School of Multidisciplinary Medical Science, University of Szeged, Szeged, H-6726, Hungary Institute of Biochemistry, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, H-6726, Hungary ProteoScientia Kft, Cserhátszentiván, H-3066, Hungary School of Ph.D. Studies, Semmelweis University, Budapest, H-1085, Hungary Biospirál-2006 Kft., Szeged, H-6726, Hungary Cited By :13 Export Date: 29 July 2021 CODEN: NARHA Correspondence Address: Welker, E.; Institute of Enzymology, Hungary; email: welker.ervin@ttk.hu Chemicals/CAS: deoxyribonuclease, 37211-67-9; CRISPR-Associated Proteins; Endodeoxyribonucleases Funding details: 3.6.3-VEKOP-16-2017-00009 Funding details: VEKOP-2.1.7-17-2016-00383 Funding details: National Research, Development and Innovation Office, 2018-1.1.1-MKI-2018-00270, K128188, PD125331 Funding text 1: National Research, Development and Innovation Office [K128188 to E.W., PD125331 to E.T and 2018-1.1.1-MKI-2018-00270]; Ministry of National Economy [VEKOP-2.1.7-17-2016-00383]; E.V. and Z.L. were supported by EFOP [3.6.3-VEKOP-16-2017-00009] grant. Funding for open access charge: National Research, Development and Innovation Office [K128188]. Conflict of interest statement. The authors are inventors on patent applications covering the LbCas12a variants described in this work. 0 false 2026-10-20T06:59:31.640+0000 2025-08-26T12:39:43.041+0000 2020-03-03T11:18:39.203+0000 true 10038471 Kulcsár Péter István /api/author/10038471 Author Péter István Kulcsár (Molekuláris biológia) true 10038471 2025-10-15T09:26:19.254+0000 2025-10-15T09:26:19.254+0000 2025-08-26T12:39:43.299+0000 true 10082735 Tóth-Lipták Péter /api/admin/10082735 Admin Péter Tóth-Lipták (SZTE admin5) true true false true 24 24 /api/publicationtype/24 PublicationType Journal Article 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType Journal Article 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType Article (Journal Article) 101 true 10000059 true 1 /api/category/1 Category Scientific true 1 Tóth, Eszter true true /api/journal/3431 REVIEWED NUCLEIC ACIDS RESEARCH 0305-1048 1362-4962 true false 3431 false 3431 true 0305-1048 1362-4962 Journal FOREIGN 48 7 3722 3733 3722 12 2020 The widespread use of Cas12a (formerly Cpf1) nucleases for genome engineering is limited by their requirement for a rather long TTTV protospacer adjacent motif (PAM) sequence. Here we have aimed to loosen these PAM constraints and have generated new PAM mutant variants of the four Cas12a orthologs that are active in mammalian and plant cells, by combining the mutations of their corresponding RR and RVR variants with altered PAM specificities. LbCas12a-RVRR showing the highest activity was selected for an in-depth characterization of its PAM preferences in mammalian cells, using a plasmid-based assay. The consensus PAM sequence of LbCas12a-RVRR resembles a TNTN motif, but also includes TACV, TTCV CTCV and CCCV. The D156R mutation in improved LbCas12a (impLbCas12a) was found to further increase the activity of that variant in a PAM-dependent manner. Due to the overlapping but still different PAM preferences of impLbCas12a and the recently reported enAsCas12a variant, they complement each other to provide increased efficiency for genome editing and transcriptome modulating applications. true true 2020 true true true false true false GOLD 2026-10-20 true https://doi.org/10.1093/nar/gkaa110 127 0 127 126 1 126 126 121 120 127 127 126 126 126 1 0 127 126 127 126 0 4 4 Folyóiratcikk Journal Article 24 124 Könyvrészlet Chapter in Book 25 3 Könyv Book 23 0 Egyéb konferenciaközlemény Conference paper 31 0 Egyéb konferenciakötet Conference proceedings 32 0 Oltalmi formák Protection forms 26 0 Disszertáció Thesis 28 0 Egyéb Miscellaneous 29 0 Alkotás Achievement 22 0 Kutatási adat Research data 33 0 Folyóiratcikk Journal Article 24 0 0 0 Könyvrészlet Chapter in Book 25 0 0 0 Könyv Book 23 0 0 0 Egyéb konferenciaközlemény Conference paper 31 0 0 0 Egyéb konferenciakötet Conference proceedings 32 0 0 0 Oltalmi formák Protection forms 26 0 0 0 Disszertáció Thesis 28 0 0 0 Egyéb Miscellaneous 29 0 0 0 Alkotás Achievement 22 0 0 0 Kutatási adat Research data 33 0 0 0 2020 0 7 7 7 7 2021 0 10 9 10 9 2022 0 20 20 20 20 2023 0 23 23 23 23 2024 0 21 21 21 21 2025 0 29 29 29 29 2026 0 17 17 17 17 D1 false 10020968,10001345,10055848,10038471 false false false 2020-06-01T10:19:01.439+0000 Biokémiai Intézet (HRN SZBK); Doktori Iskola (SE); Enzimológiai Intézet (TTK); Multidiszciplináris Orvostudományok Doktori Iskola (SZTE / DI) 9 32 4 11 0 true Language 10002 /api/language/10002 English Angol English true 2 true PersonAuthorship 90804007 /api/authorship/90804007 Tóth, Eszter [Tóth, Eszter (molekuláris biológia), author] 1 0.09090909 true false false Author 10020968 /api/author/10020968 Eszter Tóth (molekuláris biológia) Tóth Eszter true 10020968 true Tóth Eszter true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false true PersonAuthorship 90804008 /api/authorship/90804008 Varga, Éva [Varga, Éva (Géntechnológia, b...), author] Institute of Biochemistry; Institute of Enzymology (RCNS); Doctoral School of Multidisciplinary Medicine (SZTE / DI) 2 0.091 false false false Author 10078525 /api/author/10078525 Éva Varga (Géntechnológia, biológia) Varga Éva true true Varga Éva true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false true PersonAuthorship 90804009 /api/authorship/90804009 Kulcsár, Péter István [Kulcsár, Péter István (Molekuláris biológia), author] Institute of Biochemistry; Doctoral School of Multidisciplinary Medicine (SZTE / DI) 3 0.09090909 false false false Author 10038471 /api/author/10038471 Péter István Kulcsár (Molekuláris biológia) Kulcsár Péter István true 10038471 true Kulcsár Péter István true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false true PersonAuthorship 90804010 /api/authorship/90804010 Kocsis-Jutka, Virág 4 0.09090909 false false false Kocsis-Jutka Virág true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false true PersonAuthorship 90804011 /api/authorship/90804011 Krausz, Sarah Laura [Krausz, Sarah (géntechnológia), author] School of PhD Studies (SU) 5 0.09090909 false false false Author 10061281 /api/author/10061281 Sarah Krausz (géntechnológia) Krausz Sarah true 10061281 true Krausz Sarah Laura true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false true PersonAuthorship 90804012 /api/authorship/90804012 Nyeste, Antal [Nyeste, Antal (Biológus), author] Institute of Biochemistry 6 0.09090909 false false false Author 10039251 /api/author/10039251 Antal Nyeste (Biológus) Nyeste Antal true 10039251 true Nyeste Antal true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false true PersonAuthorship 90804013 /api/authorship/90804013 Welker, Zsombor 7 0.09090909 false false false Welker Zsombor true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false true PersonAuthorship 90804014 /api/authorship/90804014 Huszár, Krisztina [Huszár, Krisztina (molekuláris biológia), author] Institute of Enzymology (RCNS) 8 0.09090909 false false false Author 10039659 /api/author/10039659 Krisztina Huszár (molekuláris biológia) Huszár Krisztina true 10039659 true Huszár Krisztina true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false true PersonAuthorship 90804015 /api/authorship/90804015 Ligeti, Zoltán [Ligeti, Zoltán Mihály (Biológia), author] Institute of Biochemistry; Doctoral School of Multidisciplinary Medicine (SZTE / DI) 9 0.09090909 false false false Author 10069721 /api/author/10069721 Zoltán Mihály Ligeti (Biológia) Ligeti Zoltán Mihály true true Ligeti Zoltán true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false true PersonAuthorship 90804016 /api/authorship/90804016 Tálas, András [Tálas, András (Molekuláris Biológus), author] Institute of Enzymology (RCNS); School of PhD Studies (SU) 10 0.09090909 false false false Author 10055848 /api/author/10055848 András Tálas (Molekuláris Biológus) Tálas András true 10055848 true Tálas András true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false true PersonAuthorship 90804017 /api/authorship/90804017 Welker, Ervin ✉ [Welker, Ervin (Fehérje feltekeredés), author] Institute of Biochemistry; Institute of Enzymology (RCNS) 11 0.09090909 false true true Author 10001345 /api/author/10001345 Ervin Welker (Fehérje feltekeredés) Welker Ervin true 10001345 true Welker Ervin true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false true PublicationIdentifier 16681148 /api/publicationidentifier/16681148 DOI: 10.1093/nar/gkaa110 PlainSource 6 /api/publicationsource/6 DOI PublicationSourceType 10001 /api/publicationsourcetype/10001 DOI true true true DOI DOI https://doi.org/@@@ true true 6 true GOLD true IDENTICAL 10.1093/nar/gkaa110 https://doi.org/10.1093/nar/gkaa110 false true PublicationIdentifier 16755828 /api/publicationidentifier/16755828 REAL: 107193 SwordSource 36 /api/publicationsource/36 REAL PublicationSourceType 10007 /api/publicationsourcetype/10007 Repozitórium true true true REAL REAL https://real.mtak.hu/@@@ true true 36 true GOLD true 107193 https://real.mtak.hu/107193 false true PublicationIdentifier 16905144 /api/publicationidentifier/16905144 WoS: 000525957700027 PlainSource 1 /api/publicationsource/1 WoS PublicationSourceType 10003 /api/publicationsourcetype/10003 Indexelő adatbázis false true true WoS WoS https://www.webofscience.com/wos/woscc/full-record/@@@ true true 1 true IDENTICAL 000525957700027 https://www.webofscience.com/wos/woscc/full-record/000525957700027 false true PublicationIdentifier 18389183 /api/publicationidentifier/18389183 Scopus: 85083539985 PlainSource 3 /api/publicationsource/3 Scopus PublicationSourceType 10003 /api/publicationsourcetype/10003 Indexelő adatbázis false true true Scopus Scopus http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-@@@ true true 3 true IDENTICAL 85083539985 http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85083539985 true true PublicationIdentifier 16755816 /api/publicationidentifier/16755816 PubMed: 32107556 PlainSource 17 /api/publicationsource/17 PubMed PublicationSourceType 10003 /api/publicationsourcetype/10003 Indexelő adatbázis false true true PubMed PubMed http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=@@@&dopt=Abstract true true 17 true IDENTICAL 32107556 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=32107556&dopt=Abstract false true PublicationIdentifier 16681149 /api/publicationidentifier/16681149 Egyéb URL: https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkaa110/5763098 PlainSource 40 /api/publicationsource/40 Other URL PublicationSourceType 10006 /api/publicationsourcetype/10006 Link true true true Egyéb URL Other URL @@@ true true 40 true https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkaa110/5763098 https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkaa110/5763098 false true SjrRating 10806136 /api/sjrrating/10806136 sjr:D1 (2020) Scopus - Genetics NUCLEIC ACIDS RESEARCH 0305-1048 1362-4962 6 0.1 journal RatingType 10002 /api/ratingtype/10002 sjr sjr true true ClassificationExternal 1311 /api/classificationexternal/1311 Scopus - Genetics true 1311 true D1 DIRECT true true /api/publication/31203798 Tóth Eszter et al. Improved LbCas12a variants with altered PAM specificities further broaden the genome targeting range of Cas12a nucleases. (2020) NUCLEIC ACIDS RESEARCH 0305-1048 1362-4962 48 7 3722-3733<div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" mtid="10020968"><a href="/gui2/?type=authors&mode=browse&sel=10020968" target="_blank">Tóth Eszter (<span class="authorship-author-name">Tóth Eszter</span> <span class="authorAux-mtmt"> molekuláris biológia</span>) </a> </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10078525"><a href="/gui2/?type=authors&mode=browse&sel=10078525" target="_blank">Varga Éva (<span class="authorship-author-name">Varga Éva</span> <span class="authorAux-mtmt"> Géntechnológia, biológia</span>) </a> </span> <span class="author-affil"><span title="Biological Research Centre, Szeged">BRC Szeged</span>/Institute of Biochemistry; <span title="Research Centre for Natural Sciences">RCNS</span>/Institute of Enzymology; <span title="University of Szeged">SZTE</span>/<span title="Doktori Iskolák">DI</span>/Doctoral School of Multidisciplinary Medicine</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10038471"><a href="/gui2/?type=authors&mode=browse&sel=10038471" target="_blank">Kulcsár Péter István (<span class="authorship-author-name">Kulcsár Péter István</span> <span class="authorAux-mtmt"> Molekuláris biológia</span>) </a> </span> <span class="author-affil"><span title="Biological Research Centre, Szeged">BRC Szeged</span>/Institute of Biochemistry; <span title="University of Szeged">SZTE</span>/<span title="Doktori Iskolák">DI</span>/Doctoral School of Multidisciplinary Medicine</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Kocsis-Jutka Virág </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10061281"><a href="/gui2/?type=authors&mode=browse&sel=10061281" target="_blank">Krausz Sarah Laura (<span class="authorship-author-name">Krausz Sarah</span> <span class="authorAux-mtmt"> géntechnológia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis University">SU</span>/School of PhD Studies</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10039251"><a href="/gui2/?type=authors&mode=browse&sel=10039251" target="_blank">Nyeste Antal (<span class="authorship-author-name">Nyeste Antal</span> <span class="authorAux-mtmt"> Biológus</span>) </a> </span> <span class="author-affil"><span title="Biological Research Centre, Szeged">BRC Szeged</span>/Institute of Biochemistry</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Welker Zsombor </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10039659"><a href="/gui2/?type=authors&mode=browse&sel=10039659" target="_blank">Huszár Krisztina (<span class="authorship-author-name">Huszár Krisztina</span> <span class="authorAux-mtmt"> molekuláris biológia</span>) </a> </span> <span class="author-affil"><span title="Research Centre for Natural Sciences">RCNS</span>/Institute of Enzymology</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10069721"><a href="/gui2/?type=authors&mode=browse&sel=10069721" target="_blank">Ligeti Zoltán (<span class="authorship-author-name">Ligeti Zoltán Mihály</span> <span class="authorAux-mtmt"> Biológia</span>) </a> </span> <span class="author-affil"><span title="Biological Research Centre, Szeged">BRC Szeged</span>/Institute of Biochemistry; <span title="University of Szeged">SZTE</span>/<span title="Doktori Iskolák">DI</span>/Doctoral School of Multidisciplinary Medicine</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10055848"><a href="/gui2/?type=authors&mode=browse&sel=10055848" target="_blank">Tálas András (<span class="authorship-author-name">Tálas András</span> <span class="authorAux-mtmt"> Molekuláris Biológus</span>) </a> </span> <span class="author-affil"><span title="Research Centre for Natural Sciences">RCNS</span>/Institute of Enzymology; <span title="Semmelweis University">SU</span>/School of PhD Studies</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10001345"><a href="/gui2/?type=authors&mode=browse&sel=10001345" target="_blank">Welker Ervin ✉ (<span class="authorship-author-name">Welker Ervin</span> <span class="authorAux-mtmt"> Fehérje feltekeredés</span>) </a> </span> <span class="author-affil"><span title="Biological Research Centre, Szeged">BRC Szeged</span>/Institute of Biochemistry; <span title="Research Centre for Natural Sciences">RCNS</span>/Institute of Enzymology</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;31203798" target="_blank">Improved LbCas12a variants with altered PAM specificities further broaden the genome targeting range of Cas12a nucleases</a></div> <div> <span class="journal-title">NUCLEIC ACIDS RESEARCH</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/0305-1048">0305-1048</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1362-4962">1362-4962</a>)</span>: <span class="journal-volume">48</span> <span class="journal-issue">7</span> <span class="page"> pp 3722-3733 </span> <span class="year">(2020)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Language: English | </span> <span class="identifiers"> <span class="id identifier oa_GOLD" title=" Gold "> <a style="color:blue" title="10.1093/nar/gkaa110" target="_blank" href="https://doi.org/10.1093/nar/gkaa110"> DOI </a> </span> <span class="id identifier oa_GOLD" title=" Gold "> <a style="color:black" title="107193" target="_blank" href="https://real.mtak.hu/107193"> REAL </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="000525957700027" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/000525957700027"> WoS </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="85083539985" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85083539985"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="32107556" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=32107556&dopt=Abstract"> PubMed </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:black" title="https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkaa110/5763098" target="_blank" href="https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkaa110/5763098"> Other URL </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Journal subject: Scopus - Genetics&nbsp;&nbsp;&nbsp;Rank:&nbsp;D1</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="" class="citingPub-count">Citing papers: 127</span> | Independent citation: 126 | Self citation: 1 | Unknown citation: 0 | Number of citations in WoS: 121 | Number of citations in Scopus:&nbsp;120 | WoS/Scopus assigned:&nbsp;127 | Number of citations with DOI:&nbsp;126 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;31203798&sort=publishedYear,desc&sort=title"> Number of cited publications: 4 </a> </div> <div class="mtid"><span class="long-pub-mtid">Publication: 31203798</span> | <span class="status-data status-ADMIN_APPROVED"> Admin approved </span> Core Citing | <span class="type-subtype">Journal Article ( Article ) </span> | <span class="pub-category">Scientific</span> | <span class="publication-sourceOfData">kézi felvitel</span> </div> <div class="lastModified">Last Modified: 2025.08.26. 14:39 Péter Tóth-Lipták (SZTE admin5) </div> <pre class="comment" style="margin-top: 0; margin-bottom: 0;"><u>Comments</u>: Institute of Enzymology, Research Centre of Natural Sciences of the Hungarian Academy of Sciences, Budapest, H-1117, Hungary Doctoral School of Multidisciplinary Medical Science, University of Szeged, Szeged, H-6726, Hungary Institute of Biochemistry, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, H-6726, Hungary ...</pre> </div></div>