https://m2.mtmt.hu/ hun 2026-10-16 19:24 JournalArticle 31203798 ADMIN_APPROVED true Institute of Enzymology, Research Centre of Natural Sciences of the Hungarian Academy of Sciences, Budapest, H-1117, Hungary Doctoral School of Multidisciplinary Medical Science, University of Szeged, Szeged, H-6726, Hungary Institute of Biochemistry, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, H-6726, Hungary ProteoScientia Kft, Cserhátszentiván, H-3066, Hungary School of Ph.D. Studies, Semmelweis University, Budapest, H-1085, Hungary Biospirál-2006 Kft., Szeged, H-6726, Hungary Cited By :13 Export Date: 29 July 2021 CODEN: NARHA Correspondence Address: Welker, E.; Institute of Enzymology, Hungary; email: welker.ervin@ttk.hu Chemicals/CAS: deoxyribonuclease, 37211-67-9; CRISPR-Associated Proteins; Endodeoxyribonucleases Funding details: 3.6.3-VEKOP-16-2017-00009 Funding details: VEKOP-2.1.7-17-2016-00383 Funding details: National Research, Development and Innovation Office, 2018-1.1.1-MKI-2018-00270, K128188, PD125331 Funding text 1: National Research, Development and Innovation Office [K128188 to E.W., PD125331 to E.T and 2018-1.1.1-MKI-2018-00270]; Ministry of National Economy [VEKOP-2.1.7-17-2016-00383]; E.V. and Z.L. were supported by EFOP [3.6.3-VEKOP-16-2017-00009] grant. Funding for open access charge: National Research, Development and Innovation Office [K128188]. Conflict of interest statement. The authors are inventors on patent applications covering the LbCas12a variants described in this work. 0 false 2026-07-17T01:21:20.586+0000 2025-08-26T12:39:43.041+0000 2020-03-03T11:18:39.203+0000 true 10038471 Kulcsár Péter István /api/author/10038471 Author true 10038471 2025-10-15T09:26:19.254+0000 2025-10-15T09:26:19.254+0000 2025-08-26T12:39:43.299+0000 true 10082735 Tóth-Lipták Péter /api/admin/10082735 Admin true true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Tóth, Eszter Improved LbCas12a variants with altered PAM specificities further broaden the genome targeting range of Cas12a nucleases true true /api/journal/3431 REVIEWED true false 3431 false 3431 true 0305-1048 1362-4962 Journal FOREIGN 48 7 3722 3733 3722 12 2020 The widespread use of Cas12a (formerly Cpf1) nucleases for genome engineering is limited by their requirement for a rather long TTTV protospacer adjacent motif (PAM) sequence. Here we have aimed to loosen these PAM constraints and have generated new PAM mutant variants of the four Cas12a orthologs that are active in mammalian and plant cells, by combining the mutations of their corresponding RR and RVR variants with altered PAM specificities. LbCas12a-RVRR showing the highest activity was selected for an in-depth characterization of its PAM preferences in mammalian cells, using a plasmid-based assay. The consensus PAM sequence of LbCas12a-RVRR resembles a TNTN motif, but also includes TACV, TTCV CTCV and CCCV. The D156R mutation in improved LbCas12a (impLbCas12a) was found to further increase the activity of that variant in a PAM-dependent manner. Due to the overlapping but still different PAM preferences of impLbCas12a and the recently reported enAsCas12a variant, they complement each other to provide increased efficiency for genome editing and transcriptome modulating applications. true true 2020 true true true false true false GOLD 2026-07-17 true https://doi.org/10.1093/nar/gkaa110 121 0 121 120 1 120 120 115 120 121 121 120 120 120 1 0 121 120 121 120 0 4 4 Folyóiratcikk Journal Article 24 118 Könyvrészlet Chapter in Book 25 3 Könyv Book 23 0 Egyéb konferenciaközlemény Conference paper 31 0 Egyéb konferenciakötet Conference proceedings 32 0 Oltalmi formák Protection forms 26 0 Disszertáció Thesis 28 0 Egyéb Miscellaneous 29 0 Alkotás Achievement 22 0 Kutatási adat Research data 33 0 Folyóiratcikk Journal Article 24 0 0 0 Könyvrészlet Chapter in Book 25 0 0 0 Könyv Book 23 0 0 0 Egyéb konferenciaközlemény Conference paper 31 0 0 0 Egyéb konferenciakötet Conference proceedings 32 0 0 0 Oltalmi formák Protection forms 26 0 0 0 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src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" mtid="10020968"><a href="/gui2/?type=authors&mode=browse&sel=10020968" target="_blank">Tóth Eszter (<span class="authorship-author-name">Tóth Eszter</span> <span class="authorAux-mtmt"> molekuláris biológia</span>) </a> </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10078525"><a href="/gui2/?type=authors&mode=browse&sel=10078525" target="_blank">Varga Éva (<span class="authorship-author-name">Varga Éva</span> <span class="authorAux-mtmt"> Géntechnológia, biológia</span>) </a> </span> <span class="author-affil"><span title="Szegedi Biológiai Kutatóközpont">SZBK</span>/Biokémiai Intézet; <span title="Természettudományi Kutatóközpont">TTK</span>/Enzimológiai Intézet; <span title="Szegedi Tudományegyetem">SZTE</span>/<span title="Doktori Iskolák">DI</span>/Multidiszciplináris Orvostudományok Doktori Iskola</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10038471"><a href="/gui2/?type=authors&mode=browse&sel=10038471" target="_blank">Kulcsár Péter István (<span class="authorship-author-name">Kulcsár Péter István</span> <span class="authorAux-mtmt"> Molekuláris biológia</span>) </a> </span> <span class="author-affil"><span title="Szegedi Biológiai Kutatóközpont">SZBK</span>/Biokémiai Intézet; <span title="Szegedi Tudományegyetem">SZTE</span>/<span title="Doktori Iskolák">DI</span>/Multidiszciplináris Orvostudományok Doktori Iskola</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Kocsis-Jutka Virág </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10061281"><a href="/gui2/?type=authors&mode=browse&sel=10061281" target="_blank">Krausz Sarah Laura (<span class="authorship-author-name">Krausz Sarah</span> <span class="authorAux-mtmt"> géntechnológia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/Doktori Iskola</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10039251"><a href="/gui2/?type=authors&mode=browse&sel=10039251" target="_blank">Nyeste Antal (<span class="authorship-author-name">Nyeste Antal</span> <span class="authorAux-mtmt"> Biológus</span>) </a> </span> <span class="author-affil"><span title="Szegedi Biológiai Kutatóközpont">SZBK</span>/Biokémiai Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Welker Zsombor </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10039659"><a href="/gui2/?type=authors&mode=browse&sel=10039659" target="_blank">Huszár Krisztina (<span class="authorship-author-name">Huszár Krisztina</span> <span class="authorAux-mtmt"> molekuláris biológia</span>) </a> </span> <span class="author-affil"><span title="Természettudományi Kutatóközpont">TTK</span>/Enzimológiai Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10069721"><a href="/gui2/?type=authors&mode=browse&sel=10069721" target="_blank">Ligeti Zoltán (<span class="authorship-author-name">Ligeti Zoltán Mihály</span> <span class="authorAux-mtmt"> Biológia</span>) </a> </span> <span class="author-affil"><span title="Szegedi Biológiai Kutatóközpont">SZBK</span>/Biokémiai Intézet; <span title="Szegedi Tudományegyetem">SZTE</span>/<span title="Doktori Iskolák">DI</span>/Multidiszciplináris Orvostudományok Doktori Iskola</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10055848"><a href="/gui2/?type=authors&mode=browse&sel=10055848" target="_blank">Tálas András (<span class="authorship-author-name">Tálas András</span> <span class="authorAux-mtmt"> Molekuláris Biológus</span>) </a> </span> <span class="author-affil"><span title="Természettudományi Kutatóközpont">TTK</span>/Enzimológiai Intézet; <span title="Semmelweis Egyetem">SE</span>/Doktori Iskola</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10001345"><a href="/gui2/?type=authors&mode=browse&sel=10001345" target="_blank">Welker Ervin ✉ (<span class="authorship-author-name">Welker Ervin</span> <span class="authorAux-mtmt"> Fehérje feltekeredés</span>) </a> </span> <span class="author-affil"><span title="Szegedi Biológiai Kutatóközpont">SZBK</span>/Biokémiai Intézet; <span title="Természettudományi Kutatóközpont">TTK</span>/Enzimológiai Intézet</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;31203798" target="_blank">Improved LbCas12a variants with altered PAM specificities further broaden the genome targeting range of Cas12a nucleases</a></div> <div> <span class="journal-title">NUCLEIC ACIDS RESEARCH</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/0305-1048">0305-1048</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1362-4962">1362-4962</a>)</span>: <span class="journal-volume">48</span> <span class="journal-issue">7</span> <span class="page"> pp 3722-3733 </span> <span class="year">(2020)</span> </div> <div class="pub-footer"> <span class="language" 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target="_blank" href="/api/publication?cond=citations.related;eq;31203798&sort=publishedYear,desc&sort=title"> Idézett közlemények száma: 4 </a> </div> <div class="mtid"><span class="long-pub-mtid">Közlemény: 31203798</span> | <span class="status-data status-ADMIN_APPROVED"> Admin láttamozott </span> Forrás Idéző | <span class="type-subtype">Folyóiratcikk ( Szakcikk ) </span> | <span class="pub-category">Tudományos</span> | <span class="publication-sourceOfData">kézi felvitel</span> </div> <div class="lastModified">Utolsó módosítás: 2025.08.26. 14:39 Tóth-Lipták Péter (SZTE admin5) </div> <pre class="comment" style="margin-top: 0; margin-bottom: 0;"><u>Megjegyzés</u>: Institute of Enzymology, Research Centre of Natural Sciences of the Hungarian Academy of Sciences, Budapest, H-1117, Hungary Doctoral School of Multidisciplinary Medical Science, University of Szeged, Szeged, H-6726, Hungary Institute of Biochemistry, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, H-6726, Hungary ...</pre> </div></div>