This volume presents valuable techniques for studying the class of ligand-activated
transcription factors known as nuclear receptors. After a brief overview of the history
of the field, chapters cover methods to detect the receptors and their mRNAs in various
tissues, protocols to characterize nuclear receptor modulators and activities, their
signaling and roles in certain pathogenesis, molecular modeling of nuclear receptor-ligand
interactions, as well as the utility of informatics in the field of nuclear receptors.
Written in the highly successful Methods in Molecular Biology series format, chapters
include introductions to their respective topics, lists of the necessary materials
and reagents, step-by-step, readily reproducible laboratory protocols, and tips on
troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Nuclear Receptors: Methods and Experimental Protocols
seeks to aid researchers working toward furthering ourunderstanding of these vital
receptors and their role in numerous pathological conditions.
