Oxysterol-binding protein (OSBP)-related proteins (ORPs) mediate non-vesicular lipid-transfer
between intracellular membranes. Phosphoinositide gradients play important roles in
the ability of OSBP and some ORPs to transfer cholesterol and phosphatidylserine between
the ER and other organelle membranes. Here we show that PM association of ORP3, a
less characterized ORP family member, is triggered by PKC activation, especially when
combined with Ca2+ increases and is determined by both PI(4,5)P2 and PI4P After activation,
ORP3 efficiently extracts PI4P and to a small extent phosphatidic acid from the PM
and slightly increases PM cholesterol levels. Full activation of ORP3 results in decreased
PM PI4P levels and inhibits Ca2+ entry via the store-operated Ca2+ entry pathway.
The C-terminal region of ORP3 that follows the strictly defined lipid transfer domain,
is found to be critical for the proper localization and function of the protein.