Usp14 is required for spermatogenesis and ubiquitin stress responses in Drosophila melanogaster.

Kovács, Levente [Kovács, Levente (biokémia), szerző] Biokémiai Intézet (SZBK); Genetikai Tanszék (SZTE / TTIK / BI); Nagy, Ágota [Nagy, Ágota (genetika), szerző] Biokémiai Intézet (SZBK); Genetikai Tanszék (SZTE / TTIK / BI); Pál, Margit [Pál, Margit (biokémia), szerző] Biokémiai Intézet (SZBK); Genetikai Tanszék (SZTE / TTIK / BI); Deák, Peter ✉ [Deák, Péter (genetika), szerző] Biokémiai Intézet (SZBK); Genetikai Tanszék (SZTE / TTIK / BI)

Angol nyelvű Tudományos Szakcikk (Folyóiratcikk)
Megjelent: JOURNAL OF CELL SCIENCE 0021-9533 1477-9137 133 (2) Paper: jcs237511 , 6 p. 2020
  • SJR Scopus - Cell Biology: Q1
    Deubiquitylating (DUB) enzymes free covalently linked ubiquitin moieties from ubiquitin-ubiquitin and ubiquitin-protein conjugates, and thereby maintain the equilibrium between free and conjugated ubiquitin moieties and regulate ubiquitin-mediated cellular processes. Here, we performed genetic analyses of mutant phenotypes in Drosophila melanogaster and demonstrate that loss of Usp14 function results in male sterility, with defects in spermatid individualization and reduced testicular free monoubiquitin levels. These phenotypes were rescued by germline-specific overexpression of wild-type Usp14. Synergistic genetic interactions with Ubi-p63E and cycloheximide sensitivity suggest that ubiquitin shortage is a primary cause of male sterility. In addition, Usp14 is predominantly expressed in testes in Drosophila, indicating a higher demand for this DUB in testes that is also reflected by testis-specific loss-of-function Usp14 phenotypes. Collectively, these results suggest a major role of Usp14 in maintaining normal steady state free monoubiquitin levels during the later stages of Drosophila spermatogenesis.This article has an associated First Person interview with the first author of the paper.
    Hivatkozás stílusok: IEEEACMAPAChicagoHarvardCSLMásolásNyomtatás
    2021-08-04 10:48