„Felsőoktatási Intézményi Kiválósági Program - 4. Vízzel kapcsolatos kutatások” tématerület(NKFIH-1150-6/2019)
Támogató: NKFIH
Karotinoidok, mint a biológiai mediátorok, amelyek a fokozott vázizomfunkcióért felelősek(PD
128370) Támogató: NKFIH
Szakterületek:
Általános orvostudomány
Biokémia és molekuláris biológia
Biológiai tudományok
Fiziológia (benne citológia)
Sejtbiológia, mikrobiológia
Background: Astaxanthin (AX) a marine carotenoid is a powerful natural antioxidant
which protects against oxidative stress and improves muscle performance. Retinol and
its derivatives were described to affect lipid and energy metabolism. Up to date,
the effects of AX and retinol on excitation-contraction coupling (ECC) in skeletal
muscle are poorly described. Methods: 18 C57Bl6 mice were divided into two groups:
Control and AX supplemented in rodent chow for 4 weeks (AstaReal A1010). In vivo and
in vitro force and intracellular calcium homeostasis was studied. In some experiments
acute treatment with retinol was employed. Results: The voltage activation of calcium
transients (V50) were investigated in single flexor digitorum brevis isolated fibers
under patch clamp and no significant changes were found following AX supplementation.
Retinol shifted V50 towards more positive values and decreased the peak F/F0 of the
calcium transients. The amplitude of tetani in the extensor digitorum longus was significantly
higher in AX than in control group. Lastly, the mitochondrial calcium uptake was found
to be less prominent in AX. Conclusion: AX supplementation increases in vitro tetanic
force without affecting ECC and exerts a protecting effect on the mitochondria. Retinol
treatment has an inhibitory effect on ECC in skeletal muscle.
