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Organisms can colonize and destroy valuable cultural heritage. New advances in biotechnology and applied microbiology provide important information on conserving cultural heritage. Various physical and mechanical methods have previously been used, but they are incapable of preventing the growth of organisms entirely. Organic biocides, particularly commercial formulations, do not last long because they can be utilized as a nutrient source by indigenous microflora after these microflora are exposed to biocides and develop resistance. Therefore, inorganic nanoparticles have a better chance to protect cultural heritage. Silver (Ag2O) and titanium (TiO2) oxides are effective against biofilm, and nanoparticles of zinc oxide (ZnO) are effective antimicrobial agents. This new generation of biocides is much smaller in size and extremely active to damage DNA or RNA. In addition, green biocides from natural sources offer an alternative to chemical ones, having low toxicity compared to chemically synthesized biocides. 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