https://m2.mtmt.hu/ hun 2026-05-02 09:06 JournalArticle 3105834 VALIDATED true 0 2018-10-02T08:54:25.000+0000 false 3105834 2026-04-30T08:11:23.419+0000 2025-05-24T07:14:59.490+0000 2016-09-02T06:43:05.000+0000 true 10014559 Bolgár Andrea /api/admin/10014559 Admin true 10014559 2025-05-24T06:37:32.307+0000 2025-05-24T07:14:59.804+0000 true 10014559 Bolgár Andrea /api/admin/10014559 Admin true 10014559 true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Kovacs, B Flagellin based biomimetic coatings: From cell-repellent surfaces to highly adhesive coatings true true /api/journal/10002858 REVIEWED true false 10002858 false 10002858 true 1742-7061 1878-7568 Journal FOREIGN 42 66 76 66 11 2016 Biomimetic coatings with cell-adhesion-regulating functionalities are intensively researched today. For example, cell-based biosensing for drug development, biomedical implants, and tissue engineering require that the surface adhesion of living cells is well controlled. Recently, we have shown that the bacterial flagellar protein, flagellin, adsorbs through its terminal segments to hydrophobic surfaces, forming an oriented monolayer and exposing its variable D3 domain to the solution. Here, we hypothesized that this nanostructured layer is highly cell-repellent since it mimics the surface of the flagellar filaments. Moreover, we proposed flagellin as a carrier molecule to display the cell-adhesive RGD (Arg-Gly-Asp) peptide sequence and induce cell adhesion on the coated surface. The D3 domain of flagellin was replaced with one or more RGD motifs linked by various oligopeptides modulating flexibility and accessibility of the inserted segment. The obtained flagellin variants were applied to create surface coatings inducing cell adhesion and spreading to different levels, while wild-type flagellin was shown to form a surface layer with strong anti-adhesive properties. As reference surfaces synthetic polymers were applied which have anti-adhesive (PLL-g-PEG poly(L-lysine)-graft-poly(ethylene glycol)) or adhesion inducing properties (RGD-functionalized PLL-g-PEG). Quantitative adhesion data was obtained by employing optical biochips and microscopy. Cell-adhesion-regulating coatings can be simply formed on hydrophobic surfaces by using the developed flagellin-based constructs. The developed novel RGD-displaying flagellin variants can be easily obtained by bacterial production and can serve as alternatives to create cell-adhesion-regulating biomimetic coatings. Statement of Significance In the present work, we show for the first time that - an oriented and dense monolayer of flagellin molecules mimics the surface of the bacterial flagellar filament. Consequently, the fabricated layer is completely cell repellent.- By genetically modifying flagellin, we incorporate cell adhesion regulating functionalities into this anti-adhesive coating.- We can easily tune the adhesion of living cells from completely cell repellent to highly adhesive. © 2016 Acta Materialia Inc. 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2 2020 0 1 0 1 0 2021 0 5 4 5 4 2022 0 2 2 2 2 2023 0 3 0 3 0 2024 0 0 0 0 0 2025 0 2 2 2 2 D1 false 10013527,10043986,10001792 false false false 2017-05-17T08:33:20.000+0000 2016-09-02T06:47:13.000+0000 Biológiai Fizika Tanszék (ELTE / TTK / FizCsill_I); Fotonika Laboratórium (HUN-REN EK / MFA); Lendület Nanobioszenzorika Kutatócsoport (HUN-REN EK / MFA); Mikrotechnológia Laboratórium (HUN-REN EK / MFA); Műszaki Fizikai és Anyagtudományi Intézet (HUN-REN EK); Vékonyrétegfizika Laboratórium (HUN-REN EK / MFA) 9 52 6 10 0 true Language 10002 /api/language/10002 Angol English true 2 true PersonAuthorship 8200206 /api/authorship/8200206 1 0.1 true false Author 10043986 /api/author/10043986 Kovács Boglárka true 10043986 true Kovacs B true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 24715865 true PersonAuthorship 8200207 /api/authorship/8200207 2 0.1 false false Author 10034460 /api/author/10034460 Patkó Dániel true 10034460 true Patko D true false false 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24715860 true PersonAuthorship 8200212 /api/authorship/8200212 7 0.1 false false Author 10001792 /api/author/10001792 Nguyen Quoc Khánh true 10001792 true Khanh NQ true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 24715859 true PersonAuthorship 8200213 /api/authorship/8200213 8 0.1 false false Toth B true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 24715858 true PersonAuthorship 8200214 /api/authorship/8200214 9 0.1 false false Author 10001580 /api/author/10001580 Vonderviszt Ferenc true 10001580 true Vonderviszt F true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 24715857 true PersonAuthorship 8200215 /api/authorship/8200215 10 0.1 false true Author 10002473 /api/author/10002473 Horváth Róbert true 10002473 true Horvath R true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 24715856 true PublicationIdentifier 1122565 /api/publicationidentifier/1122565 PlainSource 6 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2125360 /api/reference/2125360 53 false true Reference 2125361 /api/reference/2125361 54 false true /api/publication/3105834 <div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" mtid="10043986"><a href="/gui2/?type=authors&mode=browse&sel=10043986" target="_blank">Kovacs B (<span class="authorship-author-name">Kovács Boglárka</span> <span class="authorAux-mtmt"> nanobioszenzorika</span>) </a> </span> <span class="author-affil"><span title="MTA Energiatudományi Kutatóközpont">MTA EK</span>/Műszaki Fizikai és Anyagtudományi Intézet; <span title="MTA Energiatudományi Kutatóközpont">MTA EK</span>/<span title="Műszaki Fizikai és Anyagtudományi Intézet">MFA</span>/Fotonika Laboratórium</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10034460"><a href="/gui2/?type=authors&mode=browse&sel=10034460" target="_blank">Patko D (<span class="authorship-author-name">Patkó Dániel</span> <span class="authorAux-mtmt"> biofizika</span>) </a> </span> <span class="author-affil"><span title="MTA Energiatudományi Kutatóközpont">MTA EK</span>/<span title="Műszaki Fizikai és Anyagtudományi Intézet">MFA</span>/Fotonika Laboratórium</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="183"><a href="/gui2/?type=authors&mode=browse&sel=183" target="_blank">Szekacs I (<span class="authorship-author-name">Székács Inna</span> <span class="authorAux-mtmt"> nanobioszenzorika</span>) </a> </span> <span class="author-affil"><span title="MTA Energiatudományi Kutatóközpont">MTA EK</span>/<span title="Műszaki Fizikai és Anyagtudományi Intézet">MFA</span>/Fotonika Laboratórium</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10035179"><a href="/gui2/?type=authors&mode=browse&sel=10035179" target="_blank">Orgovan N (<span class="authorship-author-name">Orgován Norbert</span> <span class="authorAux-mtmt"> biofizika</span>) </a> </span> <span class="author-affil"><span title="Eötvös Loránd Tudományegyetem">ELTE</span>/<span title="Természettudományi Kar">TTK</span>/<span title="Fizikai Intézet">Fiz_I</span>/Biológiai Fizika Tanszék; <span title="MTA Energiatudományi Kutatóközpont">MTA EK</span>/<span title="Műszaki Fizikai és Anyagtudományi Intézet">MFA</span>/Fotonika Laboratórium</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10013527"><a href="/gui2/?type=authors&mode=browse&sel=10013527" target="_blank">Kurunczi S (<span class="authorship-author-name">Kurunczi Sándor</span> <span class="authorAux-mtmt"> Bioszenzorika</span>) </a> </span> <span class="author-affil"><span title="MTA Energiatudományi Kutatóközpont">MTA EK</span>/<span title="Műszaki Fizikai és Anyagtudományi Intézet">MFA</span>/Fotonika Laboratórium</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10002024"><a href="/gui2/?type=authors&mode=browse&sel=10002024" target="_blank">Sulyok A (<span class="authorship-author-name">Sulyok Attila</span> <span class="authorAux-mtmt"> Felület fizika</span>) </a> </span> <span class="author-affil"><span title="MTA Energiatudományi Kutatóközpont">MTA EK</span>/<span title="Műszaki Fizikai és Anyagtudományi Intézet">MFA</span>/Vékonyrétegfizika Laboratórium</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10001792"><a href="/gui2/?type=authors&mode=browse&sel=10001792" target="_blank">Khanh NQ (<span class="authorship-author-name">Nguyen Quoc Khánh</span> <span class="authorAux-mtmt"> Nanotechnológia, ionsugaras technológia</span>) </a> </span> <span class="author-affil"><span title="MTA Energiatudományi Kutatóközpont">MTA EK</span>/<span title="Műszaki Fizikai és Anyagtudományi Intézet">MFA</span>/Mikrotechnológia Laboratórium</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Toth B </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10001580"><a href="/gui2/?type=authors&mode=browse&sel=10001580" target="_blank">Vonderviszt F (<span class="authorship-author-name">Vonderviszt Ferenc</span> <span class="authorAux-mtmt"> Molekuláris biofizika</span>) </a> </span> <span class="author-affil"><span title="MTA Energiatudományi Kutatóközpont">MTA EK</span>/<span title="Műszaki Fizikai és Anyagtudományi Intézet">MFA</span>/Fotonika Laboratórium</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10002473"><a href="/gui2/?type=authors&mode=browse&sel=10002473" target="_blank">Horvath R (<span class="authorship-author-name">Horváth Róbert</span> <span class="authorAux-mtmt"> Bioszenzorok</span>) </a> </span> <span class="author-affil"><span title="MTA Energiatudományi Kutatóközpont">MTA EK</span>/<span title="Műszaki Fizikai és Anyagtudományi Intézet">MFA</span>/Lendület Nanobioszenzorika Kutatócsoport; <span title="MTA Energiatudományi Kutatóközpont">MTA EK</span>/<span title="Műszaki Fizikai és Anyagtudományi Intézet">MFA</span>/Fotonika Laboratórium</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;3105834" target="_blank">Flagellin based biomimetic coatings: From cell-repellent surfaces to highly adhesive coatings</a></div> <div> <span class="journal-title">ACTA BIOMATERIALIA</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/1742-7061">1742-7061</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1878-7568">1878-7568</a>)</span>: <span class="journal-volume">42</span> <span class="page"> pp 66-76 </span> <span class="year">(2016)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Nyelv: Angol | </span> <span class="identifiers"> <span class="id identifier oa_PAY" title=" Fizetős "> <a style="color:blue" title="10.1016/j.actbio.2016.07.002" target="_blank" href="https://doi.org/10.1016/j.actbio.2016.07.002"> DOI </a> </span> <span class="id identifier oa_CLOSED" title=" Zárt "> <a style="color:blue" title="44304" target="_blank" href="http://real.mtak.hu/44304"> REAL </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="000383292700006" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/000383292700006"> WoS </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="84983372556" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-84983372556"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="27381523" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=27381523&dopt=Abstract"> PubMed </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="789648890126462983" target="_blank" href="http://scholar.Google.com/scholar?hl=en&lr=&cluster=789648890126462983"> Google scholar </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Folyóirat szakterülete: Scopus - Biomaterials&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;D1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Biomedical Engineering&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;D1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Biotechnology&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;D1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Medicine (miscellaneous)&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;D1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Biochemistry&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Molecular Biology&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q2</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="Nyilvános idézőközlemények összesen, említések nélkül" class="citingPub-count">Nyilvános idéző összesen: 23</span> | Független: 14 | Függő: 9 | Nem jelölt: 0 | WoS jelölt: 20 | Scopus jelölt:&nbsp;20 | WoS/Scopus jelölt:&nbsp;21 | DOI jelölt:&nbsp;21 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;3105834&sort=publishedYear,desc&sort=title"> Idézett közlemények száma: 16 </a> </div> <div class="mtid"><span class="long-pub-mtid">Közlemény: 3105834</span> | <span class="status-data status-VALIDATED"> Egyeztetett </span> <span class="oldId">Régi azonosító: 3105834</span> | Forrás Idéző | <span class="type-subtype">Folyóiratcikk ( Szakcikk ) </span> | <span class="pub-category">Tudományos</span> | <span class="publication-sourceOfData">Scopus</span> </div> <div class="funder"> (K104726) Támogató: OTKA </div> <div class="lastModified">Utolsó módosítás: 2025.05.24. 09:14 Bolgár Andrea (MFA admin 4) </div> </div></div>