https://m2.mtmt.hu/ hun 2026-09-20 14:45 JournalArticle 3105028 VALIDATED true 0 2018-02-16T12:47:21.000+0000 false 3105028 2026-05-17T11:36:11.118+0000 2024-02-01T10:15:54.402+0000 2016-08-31T08:33:13.000+0000 true 426140 Ligeti Erzsébet /api/author/426140 Author true 426140 2024-02-01T09:11:19.077+0000 2024-02-01T10:15:54.632+0000 true 10015544 Csajbók Edit /api/admin/10015544 Admin true 10015544 true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Wang, LD Phosphoproteomic profiling of mouse primary HSPCs reveals new regulators of HSPC mobilization true true /api/journal/767 REVIEWED true false 767 false 767 true 0006-4971 1528-0020 Journal FOREIGN 128 11 1465 1474 1465 10 2016 Protein phosphorylation is a central mechanism of signal transduction that both positively and negatively regulates protein function. Large-scale studies of the dynamic phosphorylation states of cell signaling systems have been applied extensively in cell lines and whole tissues to reveal critical regulatory networks, and candidate-based evaluations of phosphorylation in rare cell populations have also been informative. However, application of comprehensive profiling technologies to adult stem cell and progenitor populations has been challenging, due in large part to the scarcity of such cells in adult tissues. Here, we combine multi-color flow cytometry with highly efficient three dimensional HPLC/mass spectrometry to enable quantitative phosphoproteomic analysis from 200,000 highly purified primary mouse hematopoietic stem and progenitor cells (HSPCs). Using this platform, we identify ARHGAP25 as a novel regulator of HSPC mobilization and demonstrate that ARHGAP25 phosphorylation at serine 363 is an important modulator of its function. Our approach provides a robust platform for large-scale phosphoproteomic analyses performed with limited numbers of rare progenitor cells. Data from our study comprises a new resource for understanding the molecular signaling networks that underlie hematopoietic stem cell mobilization. true 2016 639639 true true true false true false GOLD 2026-05-17 true https://doi.org/10.1182/blood-2016-05-711424 true 10031137 Sonnevend Kinga /api/admin/10031137 Admin true 10031137 20 0 19 11 5 19 19 19 19 20 19 12 11 12 8 0 20 12 19 11 0 2 2 Folyóiratcikk Journal Article 24 18 Könyvrészlet Chapter in Book 25 1 Könyv Book 23 0 Egyéb konferenciaközlemény Conference paper 31 0 Egyéb konferenciakötet Conference proceedings 32 0 Oltalmi formák Protection forms 26 0 Disszertáció Thesis 28 1 Egyéb Miscellaneous 29 0 Alkotás Achievement 22 0 Kutatási adat Research data 33 0 Folyóiratcikk Journal Article 24 0 0 0 Könyvrészlet Chapter in Book 25 0 0 0 Könyv Book 23 0 0 0 Egyéb konferenciaközlemény Conference paper 31 0 0 0 Egyéb konferenciakötet Conference proceedings 32 0 0 0 Oltalmi formák Protection forms 26 0 0 0 Disszertáció Thesis 28 0 0 0 Egyéb Miscellaneous 29 0 0 0 Alkotás Achievement 22 0 0 0 Kutatási adat Research data 33 0 0 0 2016 0 2 1 2 1 2017 0 2 2 2 2 2018 0 2 1 2 1 2019 0 2 2 2 2 2020 0 3 2 3 2 2021 0 1 1 1 1 2022 0 3 1 3 1 2023 0 1 0 1 0 2024 0 2 1 2 1 2025 0 2 1 2 1 D1 false 10023793 false false false 2017-05-30T09:13:41.000+0000 2016-08-31T08:40:35.000+0000 Élettani Intézet (SE / AOK / I) 3 7 1 11 0 true Language 10002 /api/language/10002 Angol English true 2 true PersonAuthorship 8198824 /api/authorship/8198824 1 0.09090909 true false Wang LD true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 24711346 true PersonAuthorship 8198825 /api/authorship/8198825 2 0.09090909 false false Ficarro SB true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 24711345 true PersonAuthorship 8198826 /api/authorship/8198826 3 0.09090909 false false Hutchinson JN true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 24711344 true PersonAuthorship 8198827 /api/authorship/8198827 4 0.09090909 false false Author 10023793 /api/author/10023793 Csépányi-Kömi Roland true 10023793 true Csepanyi-Komi R true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 24711343 true PersonAuthorship 8198828 /api/authorship/8198828 5 0.09090909 false false Nguyen PT true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 24711342 true PersonAuthorship 8198829 /api/authorship/8198829 6 0.09090909 false false Author 10052421 /api/author/10052421 Wisniewski Éva true 10052421 true Wisniewski E true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 24711341 true PersonAuthorship 8198830 /api/authorship/8198830 7 0.09090909 false false Sullivan J true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 24711340 true PersonAuthorship 8198831 /api/authorship/8198831 8 0.09090909 false false Hofmann O true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 24711339 true PersonAuthorship 8198832 /api/authorship/8198832 9 0.09090909 false false Author 426140 /api/author/426140 Ligeti Erzsébet true 426140 true Ligeti E true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 24711338 true PersonAuthorship 8198833 /api/authorship/8198833 10 0.09090909 false false Marto JA true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 24711337 true PersonAuthorship 8198834 /api/authorship/8198834 11 0.09090909 false true Wagers AJ true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 24711336 true PublicationIdentifier 1122016 /api/publicationidentifier/1122016 PlainSource 6 /api/publicationsource/6 PublicationSourceType 10001 /api/publicationsourcetype/10001 true true true DOI DOI https://doi.org/@@@ true true 6 true GOLD false IDENTICAL 10.1182/blood-2016-05-711424 https://doi.org/10.1182/blood-2016-05-711424 false 1416459 true PublicationIdentifier 1122018 /api/publicationidentifier/1122018 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;&nbsp;&nbsp;&nbsp; <span class="author-name" >Ficarro SB </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Hutchinson JN </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10023793"><a href="/gui2/?type=authors&mode=browse&sel=10023793" target="_blank">Csepanyi-Komi R (<span class="authorship-author-name">Csépányi-Kömi Roland</span> <span class="authorAux-mtmt"> élettan</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Élettani Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Nguyen PT </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10052421"><a href="/gui2/?type=authors&mode=browse&sel=10052421" target="_blank">Wisniewski E (<span class="authorship-author-name">Wisniewski Éva</span> <span class="authorAux-mtmt"> Sejtélettan</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Élettani Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Sullivan J </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Hofmann O </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="426140"><a href="/gui2/?type=authors&mode=browse&sel=426140" target="_blank">Ligeti E (<span class="authorship-author-name">Ligeti Erzsébet</span> <span class="authorAux-mtmt"> Sejtélettan</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Élettani Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Marto JA </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Wagers AJ </span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;3105028" target="_blank">Phosphoproteomic profiling of mouse primary HSPCs reveals new regulators of HSPC mobilization</a></div> <div> <span class="journal-title">BLOOD</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/0006-4971">0006-4971</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1528-0020">1528-0020</a>)</span>: <span class="journal-volume">128</span> <span class="journal-issue">11</span> <span class="page"> pp 1465-1474 </span> <span class="year">(2016)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Nyelv: Angol | </span> <span class="identifiers"> <span class="id identifier oa_GOLD" title=" Gold "> <a style="color:blue" title="10.1182/blood-2016-05-711424" target="_blank" href="https://doi.org/10.1182/blood-2016-05-711424"> DOI </a> </span> <span class="id identifier oa_GREEN" title=" Green "> <a style="color:black" title="74666" target="_blank" href="http://real.mtak.hu/74666"> REAL </a> </span> <span class="id identifier oa_GREEN" title=" Green "> <a 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<OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Folyóirat szakterülete: Scopus - Biochemistry&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;D1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Cell Biology&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;D1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Hematology&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;D1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Immunology&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;D1</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="Nyilvános idézőközlemények összesen, említések nélkül" class="citingPub-count">Nyilvános idéző összesen: 20</span> | Független: 12 | Függő: 8 | Nem jelölt: 0 | WoS jelölt: 19 | Scopus jelölt:&nbsp;19 | WoS/Scopus jelölt:&nbsp;20 | DOI jelölt:&nbsp;19 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;3105028&sort=publishedYear,desc&sort=title"> Idézett közlemények száma: 2 </a> </div> <div class="mtid"><span class="long-pub-mtid">Közlemény: 3105028</span> | <span class="status-data status-VALIDATED"> Egyeztetett </span> <span class="oldId">Régi azonosító: 3105028</span> | Forrás Idéző | <span class="type-subtype">Folyóiratcikk ( Szakcikk ) </span> | <span class="pub-category">Tudományos</span> | <span class="publication-sourceOfData">MEDLINE</span> </div> <div class="lastModified">Utolsó módosítás: 2024.02.01. 11:15 Csajbók Edit (SE_KK_Admin5_CSE, admin) </div> </div></div>