The organ preservation paradigm has changed following the development of new ways
to preserve organs. The use of machine perfusion to preserve organs appears to have
several advantages compared with conventional static cold storage. For liver transplants,
the temperature control provided by machine perfusion improves organ preservation.
In this experimental study, we measured the effects of different temperatures on mitochondrial
bioenergetics during the reperfusion phase. An experimental model of ex-vivo liver
transplantation was developed in Wistar rats (Rattus norvegicus). After total hepatectomy,
cold static preservation occurred at 4 degrees C and reperfusion was performed at
37 degrees C and 32 degrees C using a Langendorff system. We measured parameters associated
with mitochondrial bioenergetics in the livers. Compared with the livers that underwent
normothermic reperfusion, mild hypothermia during reperfusion caused significant increases
in the mitochondrial membrane potential, the adenosine triphosphate content, and mitochondrial
respiration, and a significant reduction in the lag phase (all P < 0.001). Mild hypothermia
during reperfusion reduced the effect of ischemia-reperfusion injury on mitochondrial
activity in liver tissue and promoted an increase in bioenergetic availability compared
with normothermic reperfusion.