Mild hypothermia during the reperfusion phase protects mitochondrial bioenergetics against ischemia-reperfusion injury in an animal model of ex-vivo liver transplantation-an experimental study

Martins, Rui Miguel ✉; Teodoro, Joao Soeiro; Furtado, Emanuel; Oliveira, Rui Caetano; Tralhao, Jose Guilherme; Rolo, Anabela Pinto; Palmeira, Carlos Marques

Angol nyelvű Tudományos Szakcikk (Folyóiratcikk)
Megjelent: INTERNATIONAL JOURNAL OF MEDICAL SCIENCES 1449-1907 16 (9) pp. 1304-1312 2019
  • SJR Scopus - Medicine (miscellaneous): Q2
    The organ preservation paradigm has changed following the development of new ways to preserve organs. The use of machine perfusion to preserve organs appears to have several advantages compared with conventional static cold storage. For liver transplants, the temperature control provided by machine perfusion improves organ preservation. In this experimental study, we measured the effects of different temperatures on mitochondrial bioenergetics during the reperfusion phase. An experimental model of ex-vivo liver transplantation was developed in Wistar rats (Rattus norvegicus). After total hepatectomy, cold static preservation occurred at 4 degrees C and reperfusion was performed at 37 degrees C and 32 degrees C using a Langendorff system. We measured parameters associated with mitochondrial bioenergetics in the livers. Compared with the livers that underwent normothermic reperfusion, mild hypothermia during reperfusion caused significant increases in the mitochondrial membrane potential, the adenosine triphosphate content, and mitochondrial respiration, and a significant reduction in the lag phase (all P < 0.001). Mild hypothermia during reperfusion reduced the effect of ischemia-reperfusion injury on mitochondrial activity in liver tissue and promoted an increase in bioenergetic availability compared with normothermic reperfusion.
    Hivatkozás stílusok: IEEEACMAPAChicagoHarvardCSLMásolásNyomtatás
    2020-08-08 22:53