https://m2.mtmt.hu/ hun 2026-05-01 06:08 JournalArticle 3095711 VALIDATED true 0 2018-03-06T07:19:06.000+0000 false 3095711 2026-04-30T08:12:20.111+0000 2025-05-24T07:14:57.803+0000 2016-07-25T12:57:09.000+0000 2025-05-24T06:37:34.065+0000 2025-05-24T07:14:58.051+0000 true 10014559 Bolgár Andrea /api/admin/10014559 Admin true 10014559 true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Orgovan, Norbert Adhesion kinetics of human primary monocytes, dendritic cells, and macrophages: Dynamic cell adhesion measurements with a label-free optical biosensor and their comparison with end-point assays true true /api/journal/10006430 REVIEWED true false 10006430 false 10006430 true 1934-8630 1559-4106 Journal FOREIGN 11 3 031001 031001 11 2016 Monocytes, dendritic cells (DCs), and macrophages (MFs) are closely related immune cells that differ in their main functions. These specific functions are, to a considerable degree, determined by the differences in the adhesion behavior of the cells. To study the inherently and essentially dynamic aspects of the adhesion of monocytes, DCs, and MFs, dynamic cell adhesion assays were performed with a high-throughput label-free optical biosensor [Epic BenchTop (BT)] on surfaces coated with either fibrinogen (Fgn) or the biomimetic copolymer PLL-g-PEG-RGD. Cell adhesion profiles typically reached their maximum at ∼60 min after cell seeding, which was followed by a monotonic signal decrease, indicating gradually weakening cell adhesion. According to the biosensor response, cell types could be ordered by increasing adherence as monocytes, MFs, and DCs. Notably, all three cell types induced a larger biosensor signal on Fgn than on PLL-g-PEG-RGD. To interpret this result, the molecular layers were characterized by further exploiting the potentials of the biosensor: by measuring the adsorption signal induced during the surface coating procedure, the authors could estimate the surface density of adsorbed molecules and, thus, the number of binding sites potentially presented for the adhesion receptors. Surfaces coated with PLL-g-PEG-RGD presented less RGD sites, but was less efficient in promoting cell spreading than those coated with Fgn; hence, other binding sites in Fgn played a more decisive role in determining cell adherence. To support the cell adhesion data obtained with the biosensor, cell adherence on Fgn-coated surfaces 30–60 min after cell seeding was measured with three complementary techniques, i.e., with (1) a fluorescence-based classical adherence assay, (2) a shear flow chamber applying hydrodynamic shear stress to wash cells away, and (3) an automated micropipette using vacuum-generated fluid flow to lift cells up. These techniques confirmed the results obtained with the high-temporal-resolution Epic BT, but could only provide end-point data. In contrast, complex, nonmonotonic cell adhesion kinetics measured by the high-throughput optical biosensor is expected to open a window on the hidden background of the immune cell–extracellular matrix interactions. 2016 true true true false true false GOLD GOLD 2026-04-30 true https://doi.org/10.1116/1.4954789 17 0 15 3 8 15 17 15 15 15 15 3 3 5 12 0 17 5 15 3 0 13 13 Folyóiratcikk Journal Article 24 15 Könyvrészlet Chapter in Book 25 0 Könyv Book 23 0 Egyéb konferenciaközlemény Conference paper 31 0 Egyéb konferenciakötet Conference proceedings 32 0 Oltalmi formák Protection forms 26 0 Disszertáció Thesis 28 2 Egyéb Miscellaneous 29 0 Alkotás Achievement 22 0 Kutatási adat Research data 33 0 Folyóiratcikk Journal Article 24 0 0 0 Könyvrészlet Chapter in Book 25 0 0 0 Könyv Book 23 0 0 0 Egyéb konferenciaközlemény Conference paper 31 0 0 0 Egyéb konferenciakötet Conference proceedings 32 0 0 0 Oltalmi formák Protection forms 26 0 0 0 Disszertáció Thesis 28 0 0 0 Egyéb Miscellaneous 29 0 0 0 Alkotás Achievement 22 0 0 0 Kutatási adat Research data 33 0 0 0 2016 0 1 1 1 1 2017 0 1 0 1 0 2018 0 5 1 5 1 2019 0 4 1 4 1 2020 0 1 0 1 0 2021 0 2 1 2 1 2022 0 1 0 1 0 2023 0 1 0 1 0 2024 0 0 0 0 0 2025 0 1 1 1 1 Q2 true 10000638,10055879 false false false 2017-02-25T09:52:22.000+0000 2016-08-09T11:41:53.000+0000 Biológiai Fizika Tanszék (ELTE / TTK / FizCsill_I); Fotonika Laboratórium (HUN-REN EK / MFA); Immunológiai Tanszék (ELTE / TTK / Bio_I); Lendület Nanobioszenzorika Kutatócsoport (HUN-REN EK / MFA); MTA-ELTE Immunológiai Kutatócsoport (ELTE / TTK / Bio_I); Műszaki Fizikai és Anyagtudományi Intézet (HUN-REN EK) 8 35 6 8 0 true Language 10002 /api/language/10002 Angol English true 2 true PersonAuthorship 8134925 /api/authorship/8134925 1 0.125 true false Author 10035179 /api/author/10035179 Orgován Norbert true 10035179 true Orgovan Norbert true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 24526387 true PersonAuthorship 8134926 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/api/authorship/8134930 6 0.125 false false Author 2054730 /api/author/2054730 Erdei Anna true 2054730 true Erdei Anna true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 24526382 true PersonAuthorship 8134931 /api/authorship/8134931 7 0.125 false false Author 10000638 /api/author/10000638 Szabó Bálint true 10000638 true Szabó Bálint true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 24526381 true PersonAuthorship 8134932 /api/authorship/8134932 8 0.125 false true Author 10002473 /api/author/10002473 Horváth Róbert true 10002473 true Horvath Robert true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 24526380 true PublicationIdentifier 1115503 /api/publicationidentifier/1115503 PlainSource 6 /api/publicationsource/6 PublicationSourceType 10001 /api/publicationsourcetype/10001 true true true DOI DOI https://doi.org/@@@ true true 6 true GOLD true IDENTICAL 10.1116/1.4954789 https://doi.org/10.1116/1.4954789 false 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style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" mtid="10035179"><a href="/gui2/?type=authors&mode=browse&sel=10035179" target="_blank">Orgovan Norbert (<span class="authorship-author-name">Orgován Norbert</span> <span class="authorAux-mtmt"> biofizika</span>) </a> </span> <span class="author-affil"><span title="MTA Energiatudományi Kutatóközpont">MTA EK</span>/Műszaki Fizikai és Anyagtudományi Intézet; <span title="MTA Energiatudományi Kutatóközpont">MTA EK</span>/<span title="Műszaki Fizikai és Anyagtudományi Intézet">MFA</span>/Fotonika Laboratórium</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10043882"><a href="/gui2/?type=authors&mode=browse&sel=10043882" target="_blank">Ungai-Salánki Rita (<span class="authorship-author-name">Salánki Rita Zsanett</span> <span class="authorAux-mtmt"> biológia</span>) </a> </span> <span class="author-affil"><span title="MTA Energiatudományi Kutatóközpont">MTA EK</span>/<span title="Műszaki Fizikai és Anyagtudományi Intézet">MFA</span>/Fotonika Laboratórium</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10055879"><a href="/gui2/?type=authors&mode=browse&sel=10055879" target="_blank">Lukácsi Szilvia (<span class="authorship-author-name">Lukácsi Szilvia Zsófia</span> <span class="authorAux-mtmt"> immunbiológia</span>) </a> </span> <span class="author-affil"><span title="Eötvös Loránd Tudományegyetem">ELTE</span>/<span title="Természettudományi Kar">TTK</span>/<span title="Biológiai Intézet">Bio_I</span>/MTA-ELTE Immunológiai Kutatócsoport</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10018292"><a href="/gui2/?type=authors&mode=browse&sel=10018292" target="_blank">Sándor Noémi (<span class="authorship-author-name">Sándor Noémi</span> <span class="authorAux-mtmt"> immunológia</span>) </a> </span> <span class="author-affil"><span title="Eötvös Loránd Tudományegyetem">ELTE</span>/<span title="Természettudományi Kar">TTK</span>/<span title="Biológiai Intézet">Bio_I</span>/MTA-ELTE Immunológiai Kutatócsoport</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10008579"><a href="/gui2/?type=authors&mode=browse&sel=10008579" target="_blank">Bajtay Zsuzsa (<span class="authorship-author-name">Bajtay Zsuzsanna</span> <span class="authorAux-mtmt"> Immunológia</span>) </a> </span> <span class="author-affil"><span title="Eötvös Loránd Tudományegyetem">ELTE</span>/<span title="Természettudományi Kar">TTK</span>/<span title="Biológiai Intézet">Bio_I</span>/Immunológiai Tanszék</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="2054730"><a href="/gui2/?type=authors&mode=browse&sel=2054730" target="_blank">Erdei Anna (<span class="authorship-author-name">Erdei Anna</span> <span class="authorAux-mtmt"> Immunológia</span>) </a> </span> <span class="author-affil"><span title="Eötvös Loránd Tudományegyetem">ELTE</span>/<span title="Természettudományi Kar">TTK</span>/<span title="Biológiai Intézet">Bio_I</span>/Immunológiai Tanszék</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10000638"><a href="/gui2/?type=authors&mode=browse&sel=10000638" target="_blank">Szabó Bálint (<span class="authorship-author-name">Szabó Bálint</span> <span class="authorAux-mtmt"> Biológiai fizika</span>) </a> </span> <span class="author-affil"><span title="Eötvös Loránd Tudományegyetem">ELTE</span>/<span title="Természettudományi Kar">TTK</span>/<span title="Fizikai Intézet">Fiz_I</span>/Biológiai Fizika Tanszék; <span title="MTA Energiatudományi Kutatóközpont">MTA EK</span>/<span title="Műszaki Fizikai és Anyagtudományi Intézet">MFA</span>/Fotonika Laboratórium</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10002473"><a href="/gui2/?type=authors&mode=browse&sel=10002473" target="_blank">Horvath Robert (<span class="authorship-author-name">Horváth Róbert</span> <span class="authorAux-mtmt"> Bioszenzorok</span>) </a> </span> <span class="author-affil"><span title="MTA Energiatudományi Kutatóközpont">MTA EK</span>/<span title="Műszaki Fizikai és Anyagtudományi Intézet">MFA</span>/Lendület Nanobioszenzorika Kutatócsoport; <span title="MTA Energiatudományi Kutatóközpont">MTA EK</span>/<span title="Műszaki Fizikai és Anyagtudományi Intézet">MFA</span>/Fotonika Laboratórium</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;3095711" target="_blank">Adhesion kinetics of human primary monocytes, dendritic cells, and macrophages: Dynamic cell adhesion measurements with a label-free optical biosensor and their comparison with end-point assays</a></div> <div> <span class="journal-title">BIOINTERPHASES</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/1934-8630">1934-8630</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1559-4106">1559-4106</a>)</span>: <span class="journal-volume">11</span> <span class="journal-issue">3</span> <span class="page"> Paper 031001. 11 p. </span> <span class="year">(2016)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Nyelv: Angol | </span> <span class="identifiers"> <span class="id identifier oa_GOLD" title=" Gold "> <a style="color:blue" title="10.1116/1.4954789" target="_blank" href="https://doi.org/10.1116/1.4954789"> DOI </a> </span> <span class="id identifier oa_CLOSED" title=" Zárt "> <a style="color:blue" title="42278" target="_blank" href="http://real.mtak.hu/42278"> REAL </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="000386586100002" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/000386586100002"> WoS </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="84977650779" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-84977650779"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="27368161" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=27368161&dopt=Abstract"> PubMed </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="18400756653026372675" target="_blank" href="http://scholar.Google.com/scholar?hl=en&lr=&cluster=18400756653026372675"> Google scholar </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Folyóirat szakterülete: Scopus - Biochemistry, Genetics and Molecular Biology (miscellaneous)&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q2</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Chemistry (miscellaneous)&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q2</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Materials Science (miscellaneous)&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q2</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Physics and Astronomy (miscellaneous)&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q2</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Biomaterials&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q3</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="Nyilvános idézőközlemények összesen, említések nélkül" class="citingPub-count">Nyilvános idéző összesen: 17</span> | Független: 5 | Függő: 12 | Nem jelölt: 0 | WoS jelölt: 15 | Scopus jelölt:&nbsp;15 | WoS/Scopus jelölt:&nbsp;15 | DOI jelölt:&nbsp;17 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;3095711&sort=publishedYear,desc&sort=title"> Idézett közlemények száma: 13 </a> </div> <div class="mtid"><span class="long-pub-mtid">Közlemény: 3095711</span> | <span class="status-data status-VALIDATED"> Egyeztetett </span> <span class="oldId">Régi azonosító: 3095711</span> | Forrás Idéző | <span class="type-subtype">Folyóiratcikk ( Szakcikk ) </span> | <span class="pub-category">Tudományos</span> | <span class="publication-sourceOfData">kézi felvitel</span> </div> <div class="lastModified">Utolsó módosítás: 2025.05.24. 09:14 Bolgár Andrea (MFA admin 4) </div> </div></div>