Alterations in ophthalmological parameters and cytokine levels in tears of patients with glaucoma

PIIk125! Alterations(in(ophthalmological(parameters(and(cytokine(levels(in(tears( of(patients(with(glaucoma( ( Eszter!Deák1,2,!Éva!Csősz1,!Adrienne!Csutak2! 1University&of&Debrecen,&Department&of&Biochemistry&and&Molecular&Biology,&Debrecen,& Hungary! 2University&of&Debrecen,&Department&of&Ophthalmology,&Debrecen,&Hungary! deak.eszter@med.unideb.hu!! ! Glaucoma! is! a! progressive,! chronic! neurodegenerative! disease! with! a! multifactorial! pathophysiology! that! affects! the! optic! nerve! and!may! cause! loss! of! function! up! to! the! blindness.!This!disease!has!several!types,!but!generally!categorized!by!the!anterior!chamber! (iridocorneal)!angle.!The!openBangle!glaucoma!(OAG)!and!the!angleBclosure!glaucoma!(ACG)! can!be!divided!into!primary!and!secondary!forms!and!also!can!be!categorized!as!acute!or! chronic.!Actually,!the!elevated!intraocular!pressure!(IOP)!is!the!only!risk!factor!that!can!be! influenced!with!therapy!such!as!medications!or!glaucoma!surgery.!In!routine!clinical!use,! different! classification! systems! are! used! to! evaluate! and! quantify! the! anterior! chamber! angle.!One!of!them!is!based!on!Scheimpflug!imaging,!like!the!Oculus!Pentacam.!This!imaging! technology! provides! quantitative! data! regarding! of! anterior! chamber! characteristics! and! may!serve!as!a!useful!adjunct!for!glaucoma!diagnosis!and!monitoring!reducing!chances!of! this!error!bias.!One!of!the!advantages!of!the!Pentacam!over!other!noncontact!instruments! such!as!optical!pachymetry!is!that!the!anterior!chamber!volume!(ACV),!anterior!chamber! depth!(ACD)!and!anterior!chamber!angle!(ACA),!as!well!as!the!corneal!thickness!and!corneal! topography!can!be!obtained!at!the!same!time.! The!corneal!tear!film!contains!numerous!proteins!the!qualitative!and!quantitative!pattern!of! which!can!be!pathognomonic!in!various!ophthalmic!conditions.!The!tear!can!be!collected!via! nonBinvasive!way,!so!it!is!an!appropriate!sample!type!for!biomarker!studies.!Results!from! previous!research!showed!that!there!is!a!link!between!the!inflammatory!cytokine!level!and! the!different!type!of!glaucoma!eye!structural!changing.! Our!aim!was!to!identify!specific!correlations!between!these!parametric!imaging!changes!and! examine!how!to!correlate!with!the!proteome!changes!with!cytokine!profiles!in!glaucoma.! The!cytokine!profile!in!tear!samples!of!10!OAG,!10!ACG!patients!and!10!age!and!sexBmatched! healthy!volunteers!was!analyzed!by!multiplex!cytokine!assay.!