https://m2.mtmt.hu/ hun 2026-09-22 21:33 JournalArticle 30843995 APPROVED true 0 false 2026-05-19T12:14:48.499+0000 2022-06-13T09:13:03.554+0000 2019-10-12T08:06:49.591+0000 true 10000059 Kolev Kraszimir Nikolaev /api/author/10000059 Author true 10000059 2026-04-25T09:11:56.839+0000 2020-02-21T18:23:54.108+0000 true 10019986 Resch Miklós /api/admin/10019986 Admin true 10019986 Admin 10003132 /api/admin/10003132 Balázs András true 10003132 true 2021-06-14T13:43:13.488+0000 true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Stachon, Tanja Erhöhte NF-κB- und iNOS-Expression in Keratozyten von Keratokonuspatienten – Hinweise auf eine entzündliche Komponente? Increased NF-κB and iNOS Expression in Keratoconus Keratocytes - Hints for an Inflammatory Component? true true /api/journal/2994 REVIEWED true false 2994 false 2994 true 0023-2165 1439-3999 Journal FOREIGN 238 09 1010 1017 1010 8 2021 In recent years, there has been increasing evidence of an inflammatory component in keratoconus. A key gene in inflammatory processes is the nuclear factor kappa B (NF-κB). NF-κB is a transcription factor for the enzyme nitric oxide synthase (NOS), which is involved with the competing enzyme arginase (Arg) in inflammatory processes. The aim of this study was to analyze the isotypes of NOS and arginase, the expression of NF-κB, NOS and arginase, and the regulatory mechanism of NOS and arginase in keratocytes of keratoconus patients using the inhibitor 1400W in vitro.Human keratocytes were isolated from surgically removed corneas of 8 KC patients and 8 normal human corneal buttons and were cultured to confluence, in vitro. Quantitative PCR and Western blot analysis were performed to examine NF-κB, NOS and arginase expression in keratocytes. Nitrite and urea concentrations in the supernatant of the cells were analyzed using 0 - 40 µM 1400W iNOS inhibitor concentrations.Only the isotypes iNOS and Arg-II were detected in the keratocytes. The mRNA expression of NF-κB and iNOS were higher in KC keratocytes than in normal cells (p = 0.0135 and p = 0.0001), whereas no differences were measurable in Arg-II expression. In the WB, a higher band intensity was measurable in NF-κB (p = 0.0012), and in iNOS, no differences in band intensity could be detected. In the supernatant of the KC keratocytes, lower concentrations of nitrite and urea were measured after the addition of the inhibitor 1400W (p ≤ 0.014), but not in normal cells (p ≥ 0.178).Due to the increased expression of NF-κB and iNOS, an inflammatory component in keratoconus must be assumed. The different regulation of the KC keratocytes by the iNOS inhibitor 1400W suggests an altered metabolic activity which can be caused by inflammatory processes. Funding 2014853 /api/funding/2014853 false true true true 2019 true true true false true false PAY 2026-05-19 false 20 0 20 8 7 20 20 17 18 18 18 8 8 8 12 0 20 8 20 8 0 3 3 Folyóiratcikk Journal Article 24 17 Könyvrészlet Chapter in Book 25 3 Könyv Book 23 0 Egyéb konferenciaközlemény Conference paper 31 0 Egyéb konferenciakötet Conference proceedings 32 0 Oltalmi formák Protection forms 26 0 Disszertáció Thesis 28 0 Egyéb Miscellaneous 29 0 Alkotás Achievement 22 0 Kutatási adat Research data 33 0 Folyóiratcikk Journal Article 24 0 0 0 Könyvrészlet Chapter in Book 25 0 0 0 Könyv Book 23 0 0 0 Egyéb konferenciaközlemény Conference paper 31 0 0 0 Egyéb konferenciakötet Conference proceedings 32 0 0 0 Oltalmi formák Protection forms 26 0 0 0 Disszertáció Thesis 28 0 0 0 Egyéb Miscellaneous 29 0 0 0 Alkotás Achievement 22 0 0 0 Kutatási adat Research data 33 0 0 0 2021 0 6 0 6 0 2022 0 4 3 4 3 2023 0 3 2 3 2 2024 0 3 1 3 1 2025 0 4 2 4 2 Q3 false false false false 2020-01-10T09:07:02.601+0000 Biokémiai Tanszék (SE / AOK / I / BMBI); Biokémiai és Molekuláris Biológiai Intézet (SE / AOK / I); Orvosi Biokémiai Intézet (SE / AOK / I) 3 9 3 6 0 true 10003132 Language 10005 /api/language/10005 Német German true 5 true PersonAuthorship 88215532 /api/authorship/88215532 1 0.16666667 true false false Stachon Tanja true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 88215533 /api/authorship/88215533 2 0.16666667 false false false Latta Lorenz true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 88215534 /api/authorship/88215534 3 0.16666667 false false false Author 10000059 /api/author/10000059 Kolev Kraszimir Nikolaev true 10000059 true Kolev Krasimir true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 88215535 /api/authorship/88215535 4 0.16666667 false false false Seitz Berthold true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 88215536 /api/authorship/88215536 5 0.16666667 false false false Author 10072292 /api/author/10072292 Langenbucher Achim true true Langenbucher Achim true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 88215537 /api/authorship/88215537 6 0.16666667 false true false Author 10000048 /api/author/10000048 Szentmáry Nóra true 10000048 true Szentmáry Nóra true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PublicationIdentifier 15971209 /api/publicationidentifier/15971209 PlainSource 6 /api/publicationsource/6 PublicationSourceType 10001 /api/publicationsourcetype/10001 true true true DOI DOI https://doi.org/@@@ true true 6 true PAY IDENTICAL 10.1055/a-1002-0100 https://doi.org/10.1055/a-1002-0100 false true PublicationIdentifier 20590941 /api/publicationidentifier/20590941 PlainSource 1 /api/publicationsource/1 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true WoS WoS https://www.webofscience.com/wos/woscc/full-record/@@@ true true 1 true IDENTICAL 000750054500013 https://www.webofscience.com/wos/woscc/full-record/000750054500013 true true PublicationIdentifier 19309957 /api/publicationidentifier/19309957 PlainSource 3 /api/publicationsource/3 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true Scopus Scopus http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-@@@ true true 3 true IDENTICAL 85114675619 http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85114675619 true true PublicationIdentifier 15971208 /api/publicationidentifier/15971208 PlainSource 17 /api/publicationsource/17 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true PubMed PubMed http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=@@@&dopt=Abstract true true 17 true IDENTICAL 31600816 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=31600816&dopt=Abstract false true Classification 10575 /api/classification/10575 true true Classification 12215 /api/classification/12215 true true SjrRating 11232039 /api/sjrrating/11232039 95 0.74 journal RatingType 10002 /api/ratingtype/10002 sjr true true ClassificationExternal 2731 /api/classificationexternal/2731 true 2731 true Q3 DIRECT true true /api/publication/30843995 <div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" >Stachon Tanja </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Latta Lorenz </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10000059"><a href="/gui2/?type=authors&mode=browse&sel=10000059" target="_blank">Kolev Krasimir (<span class="authorship-author-name">Kolev Kraszimir Nikolaev</span> <span class="authorAux-mtmt"> Orvosi biokémia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Orvosi Biokémiai Intézet; <span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Biokémiai és Molekuláris Biológiai Intézet; <span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/<span title="Biokémiai és Molekuláris Biológiai Intézet">BMBI</span>/Biokémiai Tanszék</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Seitz Berthold </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10072292"><a href="/gui2/?type=authors&mode=browse&sel=10072292" target="_blank">Langenbucher Achim (<span class="authorship-author-name">Langenbucher Achim</span> <span class="authorAux-mtmt"> Ophthalmology, ophthalmic optics</span>) </a> </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10000048"><a href="/gui2/?type=authors&mode=browse&sel=10000048" target="_blank">Szentmáry Nóra (<span class="authorship-author-name">Szentmáry Nóra</span> <span class="authorAux-mtmt"> Szemészet</span>) </a> </span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;30843995" target="_blank">Erhöhte NF-κB- und iNOS-Expression in Keratozyten von Keratokonuspatienten – Hinweise auf eine entzündliche Komponente?</a></div> <div> <span class="journal-title">KLINISCHE MONATSBLATTER FUR AUGENHEILKUNDE</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/0023-2165">0023-2165</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1439-3999">1439-3999</a>)</span>: <span class="journal-volume">238</span> <span class="journal-issue">09</span> <span class="page"> pp 1010-1017 </span> <span class="year">(2021)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Nyelv: Német | </span> <span class="identifiers"> <span class="id identifier oa_PAY" title=" Fizetős "> <a style="color:blue" title="10.1055/a-1002-0100" target="_blank" href="https://doi.org/10.1055/a-1002-0100"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="000750054500013" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/000750054500013"> WoS </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="85114675619" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85114675619"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="31600816" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=31600816&dopt=Abstract"> PubMed </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Folyóirat szakterülete: Scopus - Ophthalmology&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q3</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Medicine (miscellaneous)&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q4</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="Nyilvános idézőközlemények összesen, említések nélkül" class="citingPub-count">Nyilvános idéző összesen: 20</span> | Független: 8 | Függő: 12 | Nem jelölt: 0 | WoS jelölt: 17 | Scopus jelölt:&nbsp;18 | WoS/Scopus jelölt:&nbsp;18 | DOI jelölt:&nbsp;20 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;30843995&sort=publishedYear,desc&sort=title"> Idézett közlemények száma: 3 </a> </div> <div class="mtid"><span class="long-pub-mtid">Közlemény: 30843995</span> | <span class="status-data status-APPROVED"> Nyilvános </span> Forrás Idéző | <span class="type-subtype">Folyóiratcikk ( Szakcikk ) </span> | <span class="pub-category">Tudományos</span> | <span class="publication-sourceOfData">kézi felvitel</span> </div> <div class="funder"> (NKFIH112612) Támogató: NKFIH </div> <div class="lastModified">Utolsó módosítás: 2022.06.13. 11:13 Lévayné Deseő Katalin (MTMT Központi admin) </div> <div class="lockedBy">Központi kezelésű 2021.06.14. 15:43 Balázs András (MTMT Központ, admin) </div> </div></div>