DNA methylation patterns are often altered in human cancer and aberrant methylation
is considered a hallmark of malignant transformation. Several methods have been developed
for the characterization of gene-specific and genome-wide DNA methylation patterns.
In this chapter, we describe the methylated-CpG island recovery assay (MIRA), which
is based on the high affinity of the MBD2b/MBD3L1 complex for double-stranded CpG-methylated
DNA. MIRA has been used in combination with microarray platforms to map DNA methylation
patterns across the human genome.
