A20 in dendritic cells restrains intestinal antibacterial peptide expression and preserves commensal homeostasis

Talpin, A.; Kattah, M.G.; Advincula, R.; Fadrosh, D.; Lynch, K.; LaMere, B.; Fujimura, K.E.; Nagalingam, N.A.; Malynn, B.A.; Lynch, S.V.; Ma, A.

Angol nyelvű Tudományos Szakcikk (Folyóiratcikk)
Megjelent: PLOS ONE 1932-6203 1932-6203 14 (7) Paper: 0218999 2019
  • Pedagógiai Tudományos Bizottság: A
  • Szociológiai Tudományos Bizottság: A
  • SJR Scopus - Agricultural and Biological Sciences (miscellaneous): Q1
Azonosítók
Microbial dysbiosis commonly occurs in patients with inflammatory bowel diseases (IBD). Exogenous causes of dysbiosis such as antibiotics and diet are well described, but host derived causes are understudied. A20 is a potent regulator of signals triggered by microbial pattern molecules, and A20 regulates susceptibility to intestinal inflammation in mice and in humans. We now report that mice lacking A20 expression in dendritic cells, A20FL/FL CD11c-Cre mice (or A20dDC mice), spontaneously develop colitogenic intestinal dysbiosis that is evident upon weaning and precedes the onset of colitis. Intestines from A20dDC mice express increased amounts of Reg3β and Reg3γ, but not Ang4. A20 deficient DCs promote gut microbiota perturbation in the absence of adaptive lymphocytes. Moreover, A20 deficient DCs directly induce expression of Reg3β and Reg3γ but not Ang 4 in normal intestinal epithelial cell enteroid cultures in the absence of other cell types. These findings reveal a pathophysiological pathway in which defective expression of an IBD susceptibility gene in DCs drives aberrant expression of anti-bacterial peptides and luminal dysbiosis that in turn confers host susceptibility to intestinal inflammation. © 2019 Talpin et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Hivatkozás stílusok: IEEEACMAPAChicagoHarvardCSL
2019-11-12 22:41