https://m2.mtmt.hu/ hun 2026-05-01 15:36 JournalArticle 30765679 VALIDATED true Politehnica University of Bucharest, Faculty of Applied Chemistry and Materials Science, 1-7 Gheorghe Polizu str, Bucharest, RO-011061, Romania University of Pécs, Faculty of Natural Sciences, Doctoral School of Chemistry, 6 Ifjúság str, Pécs, Hu-7624, Hungary Sapientia Hungarian University of Transylvania, Faculty of Economics, Socio-Human Science and Engineering, Department of Bioengineering, 1 Libertatii sqr, Miercurea Ciuc, RO-530104, Romania Export Date: 4 November 2019 Correspondence Address: Miklóssy, I.; Sapientia Hungarian University of Transylvania, Faculty of Economics, Socio-Human Science and Engineering, Department of Bioengineering, 1 Libertatii sqr, Romania; email: miklossyildiko@uni.sapientia.ro 0 false 2026-02-17T10:21:01.816+0000 2025-12-17T11:08:06.307+0000 2019-08-15T08:26:41.801+0000 true 10057345 Krizsán Ivett /api/admin/10057345 Admin Krizsán Ivett (PTE BTK admin 5) true 10057345 2026-01-13T11:42:03.505+0000 2026-01-13T11:42:03.505+0000 2020-09-02T10:47:10.414+0000 true 565 WoS import /api/admin/565 Admin WoS import (admin) true true false true 24 24 /api/publicationtype/24 PublicationType Folyóiratcikk 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType Folyóiratcikk 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType Szakcikk (Folyóiratcikk) 101 true 10000059 true 1 /api/category/1 Category Tudományos true 1 Sinkler, Reka true true /api/journal/10009385 REVIEWED STUDIA UNIVERSITATIS BABES-BOLYAI CHEMIA 1224-7154 2065-9520 true false 10009385 false 10009385 true 1224-7154 2065-9520 Journal FOREIGN 64 2 159 174 159 16 2019 1,4-butanediol (BDO) is an important commodity molecule that is used as a platform chemical for the production of polybutylene terephthalate (PBT), elastic fibres (Spandex) and other materials. The homologous enzyme of E. coli, succinyl-CoA synthetase (sucCD) and the heterologous malonyl-CoA reductase from Chloroflexus aurantiacus (mcr) are key enzymes in a heterologous pathway leading to BDO production, which were introduced into a genome-engineered E. coli MG1655(DE3) Delta ldhA, Delta pflB strain. Knowing that the expression of recombinant proteins and gene deletions can significantly influence cellular viability, the present study was carried out to investigate the impact of the two key enzyme expression on deletion strains, helping us to analyze the physiological changes of E. coli strains and providing directions for further optimizations in order to achieve satisfying target product yields. 2019 true true true false false NONE 2026-02-17 false 1 0 1 0 1 1 1 1 1 1 1 0 0 0 1 0 1 0 1 0 0 1 1 Folyóiratcikk Journal Article 24 1 Könyvrészlet Chapter in Book 25 0 Könyv Book 23 0 Egyéb konferenciaközlemény Conference paper 31 0 Egyéb konferenciakötet Conference proceedings 32 0 Oltalmi formák Protection forms 26 0 Disszertáció Thesis 28 0 Egyéb Miscellaneous 29 0 Alkotás Achievement 22 0 Kutatási adat Research data 33 0 Folyóiratcikk Journal Article 24 0 0 0 Könyvrészlet Chapter in Book 25 0 0 0 Könyv Book 23 0 0 0 Egyéb konferenciaközlemény Conference paper 31 0 0 0 Egyéb konferenciakötet Conference proceedings 32 0 0 0 Oltalmi formák Protection forms 26 0 0 0 Disszertáció Thesis 28 0 0 0 Egyéb Miscellaneous 29 0 0 0 Alkotás Achievement 22 0 0 0 Kutatási adat Research data 33 0 0 0 2023 0 1 0 1 0 Q4 false 10073458,10050086,10046188 false false false 2019-11-13T09:27:35.065+0000 Biomérnöki Tanszék (EMTE / CsíkszKar); Hallgatók (PTE / DI / KDI) 6 11 2 6 0 true Language 10002 /api/language/10002 Angol Angol English true 2 true PersonAuthorship 87726371 /api/authorship/87726371 Sinkler, Reka [Sinkler, Réka (Biokémia), szerző] 1 0.16666667 true false false Author 10049315 /api/author/10049315 Sinkler Réka (Biokémia) Sinkler Réka true 10049315 true Sinkler Reka true false false AuthorshipType 1 /api/authorshiptype/1 Szerző 0 true 0 true false true PersonAuthorship 87726372 /api/authorship/87726372 Both-Fodor, Marta [Balázs, Márta (Biotechnológia), szerző] 2 0.16666667 false false false Author 10073540 /api/author/10073540 Balázs Márta (Biotechnológia) Balázs Márta true true Both-Fodor Marta true false false AuthorshipType 1 /api/authorshiptype/1 Szerző 0 true 0 true false true PersonAuthorship 87726373 /api/authorship/87726373 Antal, Emoke [Antal, Emőke (Biokémia), szerző] 3 0.16666667 false false false Author 10073479 /api/author/10073479 Antal Emőke (Biokémia) Antal Emőke true true Antal Emoke true false false AuthorshipType 1 /api/authorshiptype/1 Szerző 0 true 0 true false true PersonAuthorship 87726374 /api/authorship/87726374 Bartos, Hunor [Bartos, Hunor (biotechnológia), szerző] Hallgatók (PTE / DI / KDI) 4 0.16666667 false false false Author 10073458 /api/author/10073458 Bartos Hunor (biotechnológia) Bartos Hunor true true Bartos Hunor true false false AuthorshipType 1 /api/authorshiptype/1 Szerző 0 true 0 true false true PersonAuthorship 87726375 /api/authorship/87726375 Lanyi, Szabolcs [Lányi, Szabolcs (Vegyészmérnök), szerző] 5 0.16666667 false false false Author 10046188 /api/author/10046188 Lányi Szabolcs (Vegyészmérnök) Lányi Szabolcs true 10046188 true Lanyi Szabolcs true false false AuthorshipType 1 /api/authorshiptype/1 Szerző 0 true 0 true false true PersonAuthorship 87726376 /api/authorship/87726376 Miklossy, Ildiko ✉ [Miklóssy, Ildikó (Biokémia,molekulá...), szerző] Biomérnöki Tanszék (EMTE / CsíkszKar) 6 0.16666667 false true true Author 10050086 /api/author/10050086 Miklóssy Ildikó (Biokémia,molekuláris biológia,sejtkultúrák) Miklóssy Ildikó true 10050086 true Miklossy Ildiko true false false AuthorshipType 1 /api/authorshiptype/1 Szerző 0 true 0 true false true PublicationIdentifier 15820466 /api/publicationidentifier/15820466 DOI: 10.24193/subbchem.2019.2.13 PlainSource 6 /api/publicationsource/6 DOI PublicationSourceType 10001 /api/publicationsourcetype/10001 DOI true true true DOI DOI https://doi.org/@@@ true true 6 true IDENTICAL 10.24193/subbchem.2019.2.13 https://doi.org/10.24193/subbchem.2019.2.13 false true PublicationIdentifier 15820465 /api/publicationidentifier/15820465 WoS: 000477816000014 PlainSource 1 /api/publicationsource/1 WoS PublicationSourceType 10003 /api/publicationsourcetype/10003 Indexelő adatbázis false true true WoS WoS https://www.webofscience.com/wos/woscc/full-record/@@@ true true 1 true IDENTICAL 000477816000014 https://www.webofscience.com/wos/woscc/full-record/000477816000014 false true PublicationIdentifier 16004744 /api/publicationidentifier/16004744 Scopus: 85073333999 PlainSource 3 /api/publicationsource/3 Scopus PublicationSourceType 10003 /api/publicationsourcetype/10003 Indexelő adatbázis false true true Scopus Scopus http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-@@@ true true 3 true IDENTICAL 85073333999 http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85073333999 false true Classification 10293 /api/classification/10293 Kémiai tudományok true true Keyword 4585 /api/keyword/4585 Escherichia coli true 4585 true Keyword 1128093 /api/keyword/1128093 metabolic engineering true 1128093 true Keyword 1321318 /api/keyword/1321318 1,4-BUTANEDIOL true 1321318 true Keyword 1751428 /api/keyword/1751428 heterologous enzyme true true SjrRating 10763568 /api/sjrrating/10763568 sjr:Q4 (2019) Scopus - Chemistry (miscellaneous) STUDIA UNIVERSITATIS BABES-BOLYAI CHEMIA 1224-7154 2065-9520 341 1.0 journal RatingType 10002 /api/ratingtype/10002 sjr sjr true true ClassificationExternal 1601 /api/classificationexternal/1601 Scopus - Chemistry (miscellaneous) true 1601 true Q4 DIRECT true true Reference 2514905 /api/reference/2514905 1. Miklossy, Ildiko: In silico and in vivo stability analysis of a heterologous biosynthetic pathway for 1,4-butanediol production in metabolically engineered E-coli 1 false true Reference 2514906 /api/reference/2514906 2. Jung, Won Seok: Elevated production of 3-hydroxypropionic acid by metabolic engineering of the glycerol metabolism in Escherichia coli 2 false true Reference 2514907 /api/reference/2514907 3. Zhou, Pengpeng: Butanol production from glycerol by recombinant Escherichia coli 3 false true Reference 2514908 /api/reference/2514908 4. Straathof, Adrie J. J.: Transformation of Biomass into Commodity Chemicals Using Enzymes or Cells 4 false true Reference 2514909 /api/reference/2514909 5. Gao, Chao: Production of (3S)-acetoin from diacetyl by using stereoselective NADPH-dependent carbonyl reductase and glucose dehydrogenase 5 false true Reference 2514910 /api/reference/2514910 6. Carneiro, Sonia: Metabolic responses to recombinant bioprocesses in Escherichia coli 6 false true Reference 2514911 /api/reference/2514911 7. Kittleson, Joshua T.: Successes and failures in modular genetic engineering 7 false true Reference 2514912 /api/reference/2514912 8. Yadav, Vikramaditya G.: The future of metabolic engineering and synthetic biology: Towards a systematic practice 8 false true Reference 2514913 /api/reference/2514913 9. Zeng, An-Ping: Microbial production of diols as platform chemicals: Recent progresses 9 false true Reference 2514914 /api/reference/2514914 10. Wendisch, Volker F.: Use of Glycerol in Biotechnological Applications 10 false true Reference 2514915 /api/reference/2514915 11. Yim, Harry: Metabolic engineering of Escherichia coli for direct production of 1,4-butanediol 11 false true Reference 2514916 /api/reference/2514916 12. Keasling, Jay D.: Manufacturing Molecules Through Metabolic Engineering 12 false true Reference 2514917 /api/reference/2514917 13. Scott, Matthew: Interdependence of Cell Growth and Gene Expression: Origins and Consequences 13 false true Reference 2514918 /api/reference/2514918 14. Yadav, Vikramaditya G.: Reevaluating synthesis by biology 14 false true Reference 2514919 /api/reference/2514919 15. Nielsen, David R.: Metabolic engineering of acetoin and meso-2,3-butanediol biosynthesis in E. coli 15 false true Reference 2514920 /api/reference/2514920 16. Muntendam, Remco: Perspectives and limits of engineering the isoprenoid metabolism in heterologous hosts 16 false true Reference 2514921 /api/reference/2514921 17. da Silva, Gervasio Paulo: Glycerol: A promising and abundant carbon source for industrial microbiology 17 false true Reference 2514922 /api/reference/2514922 18. Kirby, James: Biosynthesis of Plant Isoprenoids: Perspectives for Microbial Engineering 18 false true Reference 2514923 /api/reference/2514923 19. Yazdani, Syed Shams: Engineering Escherichia coli for the efficient conversion of glycerol to ethanol and co-products 19 false true Reference 2514924 /api/reference/2514924 20. Ajikumar, Parayil Kumaran: Terpenoids: Opportunities for biosynthesis of natural product drugs using engineered microorganisms 20 false true Reference 2514925 /api/reference/2514925 21. Klein-Marcuschamer, Daniel: Engineering microbial cell factories for biosynthesis of isoprenoid molecules: beyond lycopene 21 false true Reference 2514926 /api/reference/2514926 22. Carole, TM: Opportunities in the industrial biobased products industry 22 false true Reference 2514927 /api/reference/2514927 23. Datsenko, KA: One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products 23 false true /api/publication/30765679 Sinkler Reka et al. METABOLIC ENGINEERING OF E. COLI: INFLUENCE OF GENE DELETIONS AND HETEROLOGOUS GENES ON PHYSIOLOGICAL TRAITS. (2019) STUDIA UNIVERSITATIS BABES-BOLYAI CHEMIA 1224-7154 2065-9520 64 2 159-174<div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" mtid="10049315"><a href="/gui2/?type=authors&mode=browse&sel=10049315" target="_blank">Sinkler Reka (<span class="authorship-author-name">Sinkler Réka</span> <span class="authorAux-mtmt"> Biokémia</span>) </a> </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10073540"><a href="/gui2/?type=authors&mode=browse&sel=10073540" target="_blank">Both-Fodor Marta (<span class="authorship-author-name">Balázs Márta</span> <span class="authorAux-mtmt"> Biotechnológia</span>) </a> </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10073479"><a href="/gui2/?type=authors&mode=browse&sel=10073479" target="_blank">Antal Emoke (<span class="authorship-author-name">Antal Emőke</span> <span class="authorAux-mtmt"> Biokémia</span>) </a> </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10073458"><a href="/gui2/?type=authors&mode=browse&sel=10073458" target="_blank">Bartos Hunor (<span class="authorship-author-name">Bartos Hunor</span> <span class="authorAux-mtmt"> biotechnológia</span>) </a> </span> <span class="author-affil"><span title="Pécsi Tudományegyetem">PTE</span>/<span title="Doktori Iskolák">DI</span>/<span title="Kémiai Doktori Iskola">KDI</span>/Hallgatók</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10046188"><a href="/gui2/?type=authors&mode=browse&sel=10046188" target="_blank">Lanyi Szabolcs (<span class="authorship-author-name">Lányi Szabolcs</span> <span class="authorAux-mtmt"> Vegyészmérnök</span>) </a> </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10050086"><a href="/gui2/?type=authors&mode=browse&sel=10050086" target="_blank">Miklossy Ildiko ✉ (<span class="authorship-author-name">Miklóssy Ildikó</span> <span class="authorAux-mtmt"> Biokémia,molekuláris biológia,sejtkultúrák</span>) </a> </span> <span class="author-affil"><span title="Sapientia Erdélyi Magyar Tudományegyetem">EMTE</span>/<span title="Csíkszeredai Kar">CsíkszKar</span>/Biomérnöki Tanszék</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;30765679" target="_blank">METABOLIC ENGINEERING OF E. COLI: INFLUENCE OF GENE DELETIONS AND HETEROLOGOUS GENES ON PHYSIOLOGICAL TRAITS</a></div> <div> <span class="journal-title">STUDIA UNIVERSITATIS BABES-BOLYAI CHEMIA</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/1224-7154">1224-7154</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/2065-9520">2065-9520</a>)</span>: <span class="journal-volume">64</span> <span class="journal-issue">2</span> <span class="page"> pp 159-174 </span> <span class="year">(2019)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Nyelv: Angol | </span> <span class="identifiers"> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="10.24193/subbchem.2019.2.13" target="_blank" href="https://doi.org/10.24193/subbchem.2019.2.13"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="000477816000014" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/000477816000014"> WoS </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="85073333999" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85073333999"> Scopus </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Folyóirat szakterülete: Scopus - Chemistry (miscellaneous)&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q4</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="Nyilvános idézőközlemények összesen, említések nélkül" class="citingPub-count">Nyilvános idéző összesen: 1</span> | Független: 0 | Függő: 1 | Nem jelölt: 0 | WoS jelölt: 1 | Scopus jelölt:&nbsp;1 | WoS/Scopus jelölt:&nbsp;1 | DOI jelölt:&nbsp;1 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;30765679&sort=publishedYear,desc&sort=title"> Idézett közlemények száma: 1 </a> </div> <div class="mtid"><span class="long-pub-mtid">Közlemény: 30765679</span> | <span class="status-data status-VALIDATED"> Egyeztetett </span> Forrás Idéző | <span class="type-subtype">Folyóiratcikk ( Szakcikk ) </span> | <span class="pub-category">Tudományos</span> | <span class="publication-sourceOfData">WoS XML</span> </div> <div class="lastModified">Utolsó módosítás: 2025.12.17. 12:08 Kurkó Anna (Sapientia EMTE Csíkszereda admin4) </div> <pre class="comment" style="margin-top: 0; margin-bottom: 0;"><u>Megjegyzés</u>: Politehnica University of Bucharest, Faculty of Applied Chemistry and Materials Science, 1-7 Gheorghe Polizu str, Bucharest, RO-011061, Romania University of Pécs, Faculty of Natural Sciences, Doctoral School of Chemistry, 6 Ifjúság str, Pécs, Hu-7624, Hungary Sapientia Hungarian University of Transylvania, Faculty of Economics, Socio-Human Scienc...</pre> </div></div>