In the behavioral science field, many of the oldest tests have still most frequently
been used almost in the same way for decades. The subjective influence of human observer
and the large inter-observer and inter-lab differences are substantial among these
tests. This necessitates the possibility of using technological innovations for behavioral
science to obtain new parameters, results and insights as well. The light-dark box
(LDB) test is a characteristic tool used to assess anxiety in rodents. A complete
behavioral analysis (including both anxiety and locomotion parameters) is not possible
by performing traditional LDB test protocol, as it lacks the usage of a real-time
video recording of the test. In the current report, we describe an improved approach
to conduct LDB test using a real-time video tracking system.