{ "labelLang" : "hun", "responseDate" : "2024-03-28 21:15", "content" : { "otype" : "JournalArticle", "mtid" : 30487635, "status" : "VALIDATED", "published" : true, "unhandledTickets" : 0, "deleted" : false, "lastRefresh" : "2021-08-30T16:02:26.863+0000", "lastModified" : "2019-03-01T20:57:35.324+0000", "created" : "2019-03-02T06:29:25.573+0000", "creator" : { "otype" : "Admin", "mtid" : 565, "link" : "/api/admin/565", "label" : "WoS import (admin)", "familyName" : "WoS", "givenName" : "import", "published" : true, "snippet" : true }, "lastDuplumOK" : "2019-12-18T22:08:36.631+0000", "lastDuplumSearch" : "2019-12-18T22:08:36.631+0000", "validated" : "2019-03-02T06:29:27.689+0000", "validator" : { "otype" : "Admin", "mtid" : 565, "link" : "/api/admin/565", "label" : "WoS import (admin)", "familyName" : "WoS", "givenName" : "import", "published" : true, "snippet" : true }, "core" : false, "citation" : true, "publicationPending" : false, "type" : { "otype" : "PublicationType", "mtid" : 24, "link" : "/api/publicationtype/24", "label" : "Folyóiratcikk", "code" : 24, "otypeName" : "JournalArticle", "listPosition" : 1, "published" : true, "oldId" : 24, "snippet" : true }, "subType" : { "otype" : "SubType", "mtid" : 10000059, "link" : "/api/subtype/10000059", "label" : "Szakcikk (Folyóiratcikk)", "name" : "Szakcikk", "nameEng" : "Article", "docType" : { "otype" : "PublicationType", "mtid" : 24, "link" : "/api/publicationtype/24", "label" : "Folyóiratcikk", "code" : 24, "otypeName" : "JournalArticle", "listPosition" : 1, "published" : true, "oldId" : 24, "snippet" : true }, "listPosition" : 101, "published" : true, "oldId" : 10000059, "snippet" : true }, "category" : { "otype" : "Category", "mtid" : 1, "link" : "/api/category/1", "label" : "Tudományos", "published" : true, "oldId" : 1, "snippet" : true }, "languages" : [ { "otype" : "Language", "mtid" : 10002, "link" : "/api/language/10002", "label" : "Angol", "name" : "Angol", "nameEng" : "English", "published" : true, "oldId" : 2, "snippet" : true } ], "firstAuthor" : "Xu, Hui", "authorships" : [ { "otype" : "PersonAuthorship", "mtid" : 85713175, "link" : "/api/authorship/85713175", "label" : "Xu, Hui", "listPosition" : 1, "share" : 0.0, "first" : true, "last" : false, "familyName" : "Xu", "givenName" : "Hui", "authorTyped" : true, "editorTyped" : false, "otherTyped" : false, "type" : { "otype" : "AuthorshipType", "mtid" : 1, "link" : "/api/authorshiptype/1", "label" : "Szerző", "code" : 0, "published" : true, "oldId" : 0, "snippet" : true }, "published" : false, "snippet" : true }, { "otype" : "PersonAuthorship", "mtid" : 85713176, "link" : "/api/authorship/85713176", "label" : "Cao, Yu", "listPosition" : 2, "share" : 0.0, "first" : false, "last" : false, "familyName" : "Cao", "givenName" : "Yu", "authorTyped" : true, "editorTyped" : false, "otherTyped" : false, "type" : { "otype" : "AuthorshipType", "mtid" : 1, "link" : "/api/authorshiptype/1", "label" : "Szerző", "code" : 0, "published" : true, "oldId" : 0, "snippet" : true }, "published" : false, "snippet" : true }, { "otype" : "PersonAuthorship", "mtid" : 85713177, "link" : "/api/authorship/85713177", "label" : "Li, Xi", "listPosition" : 3, "share" : 0.0, "first" : false, "last" : false, "familyName" : "Li", "givenName" : "Xi", "authorTyped" : true, "editorTyped" : false, "otherTyped" : false, "type" : { "otype" : "AuthorshipType", "mtid" : 1, "link" : "/api/authorshiptype/1", "label" : "Szerző", "code" : 0, "published" : true, "oldId" : 0, "snippet" : true }, "published" : false, "snippet" : true }, { "otype" : "PersonAuthorship", "mtid" : 85713178, "link" : "/api/authorship/85713178", "label" : "Cao, Xiyue", "listPosition" : 4, "share" : 0.0, "first" : false, "last" : false, "familyName" : "Cao", "givenName" : "Xiyue", "authorTyped" : true, "editorTyped" : false, "otherTyped" : false, "type" : { "otype" : "AuthorshipType", "mtid" : 1, "link" : "/api/authorshiptype/1", "label" : "Szerző", "code" : 0, "published" : true, "oldId" : 0, "snippet" : true }, "published" : false, "snippet" : true }, { "otype" : "PersonAuthorship", "mtid" : 85713179, "link" : "/api/authorship/85713179", "label" : "Xu, Yi", "listPosition" : 5, "share" : 0.0, "first" : false, "last" : false, "familyName" : "Xu", "givenName" : "Yi", "authorTyped" : true, "editorTyped" : false, "otherTyped" : false, "type" : { "otype" : "AuthorshipType", "mtid" : 1, "link" : "/api/authorshiptype/1", "label" : "Szerző", "code" : 0, "published" : true, "oldId" : 0, "snippet" : true }, "published" : false, "snippet" : true }, { "otype" : "PersonAuthorship", "mtid" : 85713180, "link" : "/api/authorship/85713180", "label" : "Qiao, Dairong", "listPosition" : 6, "share" : 0.0, "first" : false, "last" : false, "familyName" : "Qiao", "givenName" : "Dairong", "authorTyped" : true, "editorTyped" : false, "otherTyped" : false, "type" : { "otype" : "AuthorshipType", "mtid" : 1, "link" : "/api/authorshiptype/1", "label" : "Szerző", "code" : 0, "published" : true, "oldId" : 0, "snippet" : true }, "published" : false, "snippet" : true }, { "otype" : "PersonAuthorship", "mtid" : 85713181, "link" : "/api/authorship/85713181", "label" : "Cao, Yi", "listPosition" : 7, "share" : 0.0, "first" : false, "last" : true, "familyName" : "Cao", "givenName" : "Yi", "authorTyped" : true, "editorTyped" : false, "otherTyped" : false, "type" : { "otype" : "AuthorshipType", "mtid" : 1, "link" : "/api/authorshiptype/1", "label" : "Szerző", "code" : 0, "published" : true, "oldId" : 0, "snippet" : true }, "published" : false, "snippet" : true } ], "title" : "An electrospun chitosan-based nanofibrous membrane reactor immobilized by the non-glycosylated rPGA for hydrolysis of pectin-rich biomass", "identifiers" : [ { "otype" : "PublicationIdentifier", "mtid" : 15286057, "link" : "/api/publicationidentifier/15286057", "label" : "DOI: 10.1016/j.renene.2017.08.094", "source" : { "otype" : "PlainSource", "mtid" : 6, "link" : "/api/publicationsource/6", "label" : "DOI", "type" : { "otype" : "PublicationSourceType", "mtid" : 10001, "link" : "/api/publicationsourcetype/10001", "label" : "DOI", "mayHaveOa" : true, "published" : true, "snippet" : true }, "name" : "DOI", "nameEng" : "DOI", "linkPattern" : "https://doi.org/@@@", "publiclyVisible" : true, "published" : true, "oldId" : 6, "snippet" : true }, "validState" : "IDENTICAL", "idValue" : "10.1016/j.renene.2017.08.094", "realUrl" : "https://doi.org/10.1016%2Fj.renene.2017.08.094", "published" : false, "snippet" : true }, { "otype" : "PublicationIdentifier", "mtid" : 15286056, "link" : "/api/publicationidentifier/15286056", "label" : "WoS: 000435620100047", "source" : { "otype" : "PlainSource", "mtid" : 1, "link" : "/api/publicationsource/1", "label" : "WoS", "type" : { "otype" : "PublicationSourceType", "mtid" : 10003, "link" : "/api/publicationsourcetype/10003", "label" : "Indexelő adatbázis", "mayHaveOa" : false, "published" : true, "snippet" : true }, "name" : "WoS", "nameEng" : "WoS", "linkPattern" : "http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=CustomerName&SrcApp=CustomerName&DestLinkType=FullRecord&KeyUT=@@@&DestApp=WOS", "publiclyVisible" : true, "published" : true, "oldId" : 1, "snippet" : true }, "validState" : "IDENTICAL", "idValue" : "000435620100047", "realUrl" : "http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=CustomerName&SrcApp=CustomerName&DestLinkType=FullRecord&KeyUT=000435620100047&DestApp=WOS", "published" : false, "snippet" : true } ], "journal" : { "otype" : "Journal", "mtid" : 14834, "link" : "/api/journal/14834", "label" : "RENEWABLE ENERGY 0960-1481 1879-0682", "pIssn" : "0960-1481", "eIssn" : "1879-0682", "reviewType" : "REVIEWED", "noIF" : false, "sciIndexed" : true, "scopusIndexed" : true, "lang" : "FOREIGN", "hungarian" : false, "published" : true, "oldId" : 14834, "snippet" : true }, "volume" : "126", "firstPage" : "573", "lastPage" : "582", "firstPageOrInternalIdForSort" : "573", "pageLength" : 10, "publishedYear" : 2018, "abstractText" : "Most native polygalacturonases are glycosylated. Owing to the influence of attached glycans on the function of proteins, an immobilized non-glycosylated polygalacturonase is fabricated for the degradation of pectin-rich biomass pectin into fermentable sugars. Recombinant non-glycosylated and glycosylated PGAs expressed by E. coli and P. pastoris, respectively, were prepared. As application model, recombinant polygalacturonases in immobilized enzyme membrane reactors were tested. The electrospun chitosan-based nanofibrous membrane was selected as the support matrix. The maximum enzyme loading efficiency and enzyme binding efficiency of non-glycosylated polygalacturonase and glycosylated polygalacturonase were 89.12 mg/g and 84.13% and 34.16 mg/g and 87.18%, respectively, suggesting that the glycosylation of polygalacturonases had a serious effect on its immobilization. The membranes were characterized by scanning electron microscope, Fourier transform infrared spectroscopy and X-ray diffraction. Furthermore, the glycosylation can significantly enhance the stability of recombinant polygalacturonases. Meanwhile, the glycosylation influenced their specific activity and degradation pattern. Most interesting of all, some D-galacturonic acid monomers occurred in the hydrolysate after the degradation of pectin by the non-glycosylated polygalacturonase, and the yield of reducing sugar could be 31.98 mg after 60min, with a conversion rate of 63.96%. Thus, the constructed reactor has potential in using pectin-rich biomass as the feedstock. (C) 2017 Elsevier Ltd. All rights reserved.", "subjects" : [ { "otype" : "Classification", "mtid" : 11927, "link" : "/api/classification/11927", "label" : "Környezetmérnöki tudományok", "published" : true, "snippet" : true } ], "keywords" : [ { "otype" : "Keyword", "mtid" : 1384, "link" : "/api/keyword/1384", "label" : "IMMOBILIZATION", "published" : true, "oldId" : 1384, "snippet" : true }, { "otype" : "Keyword", "mtid" : 15061, "link" : "/api/keyword/15061", "label" : "glycosylation", "published" : true, "oldId" : 15061, "snippet" : true }, { "otype" : "Keyword", "mtid" : 1054153, "link" : "/api/keyword/1054153", "label" : "Membrane reactor", "published" : true, "oldId" : 1054153, "snippet" : true }, { "otype" : "Keyword", "mtid" : 1243293, "link" : "/api/keyword/1243293", "label" : "Polygalacturonase", "published" : true, "oldId" : 1243293, "snippet" : true }, { "otype" : "Keyword", "mtid" : 1605024, "link" : "/api/keyword/1605024", "label" : "Pectin-rich biomass", "published" : true, "snippet" : true }, { "otype" : "Keyword", "mtid" : 1605025, "link" : "/api/keyword/1605025", "label" : "Electrospun chitosan-based nanofibrous", "published" : true, "snippet" : true } ], "digital" : null, "printed" : null, "sourceYear" : 2019, "foreignEdition" : true, "foreignLanguage" : true, "fullPublication" : true, "conferencePublication" : false, "nationalOrigin" : null, "missingAuthor" : false, "oaType" : "NONE", "oaCheckDate" : "2021-08-30", "oaFree" : false, "citationCount" : 0, "citationCountUnpublished" : 0, "citationCountWoOther" : 0, "independentCitCountWoOther" : 0, "doiCitationCount" : 0, "wosCitationCount" : 0, "scopusCitationCount" : 0, "independentCitationCount" : 0, "unhandledCitationCount" : 0, "citingPubCount" : 0, "independentCitingPubCount" : 0, "unhandledCitingPubCount" : 0, "citedPubCount" : 3, "citedCount" : 3, "ratings" : [ { "otype" : "SjrRating", "mtid" : 10891310, "link" : "/api/sjrrating/10891310", "label" : "sjr:Q1 (2018) Scopus - Renewable Energy, Sustainability and the Environment RENEWABLE ENERGY 0960-1481", "listPos" : 20, "rankValue" : 0.24, "type" : "journal", "ratingType" : { "otype" : "RatingType", "mtid" : 10002, "link" : "/api/ratingtype/10002", "label" : "sjr", "code" : "sjr", "published" : true, "snippet" : true }, "subject" : { "otype" : "ClassificationExternal", "mtid" : 2105, "link" : "/api/classificationexternal/2105", "label" : "Scopus - Renewable Energy, Sustainability and the Environment", "published" : true, "oldId" : 2105, "snippet" : true }, "ranking" : "Q1", "calculation" : "DIRECT", "published" : true, "snippet" : true } ], "ratingsForSort" : "Q1", "hasCitationDuplums" : false, "directInstitutesForSort" : "", "ownerAuthorCount" : 3, "ownerInstituteCount" : 8, "directInstituteCount" : 0, "authorCount" : 7, "contributorCount" : 0, "hasQualityFactor" : true, "link" : "/api/publication/30487635", "label" : "Xu Hui et al. An electrospun chitosan-based nanofibrous membrane reactor immobilized by the non-glycosylated rPGA for hydrolysis of pectin-rich biomass. (2018) RENEWABLE ENERGY 0960-1481 1879-0682 126 573-582", "template" : "