https://m2.mtmt.hu/ hun 2026-10-23 07:50 JournalArticle 30401681 VALIDATED true 0 false 2026-10-22T10:28:23.413+0000 2024-04-03T10:12:32.103+0000 2019-01-21T11:15:50.165+0000 true 10051790 Biró Orsolya /api/author/10051790 Author true 10051790 2024-04-03T10:12:24.992+0000 2024-04-03T10:12:24.992+0000 2024-04-03T10:12:32.422+0000 true 10073918 Kovács Marcell /api/admin/10073918 Admin true Admin 10073918 /api/admin/10073918 Kovács Marcell true true 2024-03-22T13:59:27.989+0000 true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Biró, Orsolya Circulating exosomal and Argonaute-bound microRNAs in preeclampsia true true /api/journal/1672 REVIEWED true false 1672 false 1672 true 0378-1119 1879-0038 Journal FOREIGN 692 138 144 138 7 2019 Introduction microRNAs (miRNAs) play important role in the regulation of placental development, and abnormal miRNA expression is associated with preeclampsia (PE). miRNAs are released from trophoblast cells to maternal blood flow, where they are highly stable, being encapsulated inside extracellular vesicles, like exosomes or bound to Argonaute proteins. In PE, placental dysfunction leads to aberrant extracellular miRNA secretion. hsa-miR-210 is a hypoxia-sensitive miRNA found to be upregulated in PE; however, it is unknown whether it is the cause or the consequence of the disease. Objective Our aim was to analyze the expression of several miRNAs, including hsa-miR-210 in placenta, exosome and Ago-bound fractions comparing normal (N) and PE pregnancies. We performed in vitro analyses of extracellular hsa-miR-210 secretion of trophoblast cell cultures (of villous and extravillous origin) under hypoxic condition. Methods PE and N placenta samples were collected from C-sections, and blood samples were drawn from each pregnant woman in the third trimester. HTR-8 and JAR cell lines were cultured in exosome-free media and treated with hypoxia-mimetic agents. Exosome and Ago-bound fractions were isolated by membrane affinity spin column method from plasma and cell media. Short RNAs were extracted from exosomes and vesicle-free fractions, and total-RNA was isolated from the placenta samples. The RNA purity and concentration were measured by spectrophotometry. Expression analysis was carried out by qPCR with specific primers to target and reference miRNAs. Results The level of hsa-miR-210 was significantly higher in PE placentas, which could cause a minor increase of exosomal and a high elevation of Ago-bound miR-210 in circulation. Hypoxia lead to intracellular hsa-miR-210 upregulation in trophoblast cell lines. In extravillous cell (HTR-8) media, only the level of exosomal hsa-miR-210 was increased but no change in Ago-bound hsa-miR-210 level was observed. In contrast, in villous cell (JAR) media, the level of exosomal hsa-miR-210 was increased and enhanced release of Ago-bound hsa-miR-210 was also observed. Conclusion Based on our data, we postulate that in PE, exosomal hsa-miR-210 is secreted actively from the trophoblast, and by intercellular communication, it may have a role in disease etiology. In addition, there is a passive release of Ago-bound hsa-miR-210 into the circulation, which may represent by-products of cell-death and is thereby a possible consequence of the disease. Funding 2038652 /api/funding/2038652 false true Funding 2038653 /api/funding/2038653 false true Funding 2038654 /api/funding/2038654 false true true true 2019 true true true false true false GREEN 2026-10-22 true https://real.mtak.hu/91832 77 0 68 65 5 67 67 58 61 63 63 60 60 73 4 0 77 73 68 65 0 2 2 Folyóiratcikk Journal Article 24 67 Könyvrészlet Chapter in Book 25 1 Könyv Book 23 0 Egyéb konferenciaközlemény Conference paper 31 0 Egyéb konferenciakötet Conference proceedings 32 0 Oltalmi formák Protection forms 26 0 Disszertáció Thesis 28 9 Egyéb Miscellaneous 29 0 Alkotás Achievement 22 0 Kutatási adat Research data 33 0 Folyóiratcikk Journal Article 24 0 0 0 Könyvrészlet Chapter in Book 25 0 0 0 Könyv Book 23 0 0 0 Egyéb konferenciaközlemény Conference paper 31 0 0 0 Egyéb konferenciakötet Conference proceedings 32 0 0 0 Oltalmi formák Protection forms 26 0 0 0 Disszertáció Thesis 28 0 0 0 Egyéb Miscellaneous 29 0 0 0 Alkotás Achievement 22 0 0 0 Kutatási adat Research data 33 0 0 0 2019 0 13 12 13 12 2020 0 11 11 11 11 2021 0 14 14 14 14 2022 0 14 14 14 14 2023 0 7 7 7 7 2024 0 10 7 10 7 2025 0 5 5 5 5 2026 0 3 3 3 3 Q1 false 10067874,10002497,10003345,10000148,10051790 false false false 2019-04-21T11:31:23.435+0000 2019-01-21T12:31:23.539+0000 Enzimológiai Intézet (TTK); Humángenetikai Tanszék (DE / ÁOK); I. Sz. 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Sz. Szülészeti és Nőgyógyászati Klinika</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10067874"><a href="/gui2/?type=authors&mode=browse&sel=10067874" target="_blank">Fóthi Ábel (<span class="authorship-author-name">Fóthi Ábel</span> <span class="authorAux-mtmt"> genetika</span>) </a> </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10044797"><a href="/gui2/?type=authors&mode=browse&sel=10044797" target="_blank">Alasztics Bálint (<span class="authorship-author-name">Alasztics Bálint</span> <span class="authorAux-mtmt"> szülészet-nőgyógyászat</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Klinikum">K</span>/I. Sz. Szülészeti és Nőgyógyászati Klinika</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10002497"><a href="/gui2/?type=authors&mode=browse&sel=10002497" target="_blank">Nagy Bálint (<span class="authorship-author-name">Nagy Bálint</span> <span class="authorAux-mtmt"> Molekuláris genetika</span>) </a> </span> <span class="author-affil"><span title="Debreceni Egyetem">DE</span>/<span title="Általános Orvostudományi Kar">ÁOK</span>/Humángenetikai Tanszék</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10000148"><a href="/gui2/?type=authors&mode=browse&sel=10000148" target="_blank">Orbán Tamás I. ✉ (<span class="authorship-author-name">Orbán Tamás I.</span> <span class="authorAux-mtmt"> Molekuláris sejtbiológia</span>) </a> </span> <span class="author-affil"><span title="Természettudományi Kutatóközpont">TTK</span>/Enzimológiai Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10003345"><a href="/gui2/?type=authors&mode=browse&sel=10003345" target="_blank">Rigó János ✉ (<span class="authorship-author-name">Rigó János</span> <span class="authorAux-mtmt"> Szülészet-nőgyógyászat</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Klinikum">K</span>/I. 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Szülészeti és Nőgyógyászati Klinika</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;30401681" target="_blank">Circulating exosomal and Argonaute-bound microRNAs in preeclampsia</a></div> <div> <span class="journal-title">GENE</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/0378-1119">0378-1119</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1879-0038">1879-0038</a>)</span>: <span class="journal-volume">692</span> <span class="page"> pp 138-144 </span> <span class="year">(2019)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Nyelv: Angol | </span> <span class="identifiers"> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="10.1016/j.gene.2019.01.012" target="_blank" href="https://doi.org/10.1016/j.gene.2019.01.012"> DOI </a> </span> <span class="id identifier oa_GREEN" title=" Green "> <a style="color:black" title="91832" target="_blank" href="https://real.mtak.hu/91832"> REAL </a> </span> <span class="id identifier oa_RESTRICTED" title=" Korlátozott "> <a style="color:blue" title="6745" target="_blank" href="https://repo.lib.semmelweis.hu/handle/123456789/6745"> SE Repozitórium </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="000460713900018" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/000460713900018"> WoS </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="85060289951" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85060289951"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="30659946" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=30659946&dopt=Abstract"> PubMed </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Folyóirat szakterülete: Scopus - Medicine (miscellaneous)&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Genetics&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q2</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="Nyilvános idézőközlemények összesen, említések nélkül" class="citingPub-count">Nyilvános idéző összesen: 77</span> | Független: 73 | Függő: 4 | Nem jelölt: 0 | WoS jelölt: 58 | Scopus jelölt:&nbsp;61 | WoS/Scopus jelölt:&nbsp;63 | DOI jelölt:&nbsp;67 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;30401681&sort=publishedYear,desc&sort=title"> Idézett közlemények száma: 2 </a> </div> <div class="mtid"><span class="long-pub-mtid">Közlemény: 30401681</span> | <span class="status-data status-VALIDATED"> Egyeztetett </span> Forrás Idéző | <span class="type-subtype">Folyóiratcikk ( Szakcikk ) </span> | <span class="pub-category">Tudományos</span> | <span class="publication-sourceOfData">kézi felvitel</span> </div> <div class="funder"> (K112112) Támogató: OTKA, (K113023) Támogató: OTKA, (VEKOP2.1.1-15-2016-00156) </div> <div class="lastModified">Utolsó módosítás: 2024.04.03. 12:12 Kovács Marcell (MTMT Közp., admin) </div> <div class="lockedBy">Központi kezelésű 2024.03.22. 14:59 Kovács Marcell (MTMT Közp., admin) </div> </div></div>